Summary
The use of Lens culinaris lectin for electron microscopic detection of D-mannose, D-glucose and N-acetyl-D-glucosamine like sites on tumor cells, erythrocytes, erythrocyte ghosts, cultured rat liver cells and various tissues of mice is demonstrated. In addition to Lens culinaris lectin-peroxidase reaction (LcL-po reaction) the preparation of active Lens culinaris lectin-ferritin conjugate are described and the specificity of cytochemical reactions are demonstrated. Furthermore experiments by immuno freeze-etching are reported for topological analysis of the lectin receptors.
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Roth, J., Thoss, K., Wagner, M. et al. Electron microscopic demonstration of cell surface carbohydrates by means of peroxidase and ferritin complexes of the Lens culinaris lection. Histochemistry 43, 275–282 (1975). https://doi.org/10.1007/BF00499709
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DOI: https://doi.org/10.1007/BF00499709