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The assay of acid phosphatase activity by means of a laser method

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Summary

A new method of quantification of the acid phosphatase activity assay by means of laser rays along with spectrography and photometry is described. Calculated ΔS values, representing differences between densities of the spectral bands corresponding to Pb and Ag account for the amount of acP activity.

The presented laser method enables a relative evaluation of the acP activity in a given region, yielding results which can be objectively expressed by figures.

A special advantage of the presented method is, that it allows to compare the individual acP activities in different regions of the investigated organ, which may be of importance for the quantitative chemoarchitectonic of the brain.

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References

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  • Gomori, P.: Microscopic histochemistry. Chicago: Univ. Press 1953.

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  • ]Kozik, M., Arciniowicz, B., Dembczyński, J.: An attempt of applying laser rays for the detection and analysis of chemical elements in tissue slices. Acta histochem. (Jena) 37, 203–205 (1970).

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Authors and Affiliations

  1. Departments of Neurology and Histology, Medical School Poznań, and Department of Physics, Polytechnical High School Poznań, Poland

    M. Kozik, J. Warchoł & B. Arcimowicz

  2. Klinika Neurologii Akademii Medycznej, ul. Przybyszewskiego, 49, Poznań, Poland

    M. Kozik

Authors
  1. M. Kozik
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  2. J. Warchoł
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  3. B. Arcimowicz
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Kozik, M., Warchoł, J. & Arcimowicz, B. The assay of acid phosphatase activity by means of a laser method. Histochemie 24, 245–250 (1970). https://doi.org/10.1007/BF00304194

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  • DOI: https://doi.org/10.1007/BF00304194

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