Summary
The present report describes the immunocytochemical localization of ornithine decarboxylase in the prepubertal rat ovary after administration of human chorionic gonadotropin (HCG). Numerous ornithine decarboxylase immunoreactive cells appeared in the thecal layer as well as in the interstitial gland tissue after treatment with HCG. The granulosa cells, the ovum and the ovarian stroma were devoid of immunoreactive ornithine decarboxylase. In contrast to the ovary of HCG-treated rats, the ovary of prepubertal rats given the vehicle alone contained only a few weakly immunoreactive cells.
Similar content being viewed by others
References
Coons AH, Leduc EH, Connolly JM (1955) Studies on antibody production. I. A method for the histochemical demonstration of specific antibody and its application to a study of the hyperimmune rabbit. J Exp Med 102:49–59
Guha SK, Jänne J (1977) Inhibition of ornithine decarboxylase in vivo in rat ovary. Biochem Biophys Res Commun 75:136–142
Guraya SS (1978) Recent advances in the morphology, histochemistry, biochemistry, and physiology of interstitial gland cells of mammalian ovary. In: Bourne GH, Danielli JF (eds) Int Rev Cytol. Academic Press, New York, pp 171–245
Henningsson S, Persson L, Rosengren E (1978) Polyamines and nucleic acids in the mouse kidney induced to growth by testosterone propionate. Acta Physiol Scand 102:385–393
Jänne J, Pösö H, Raina A (1978) Polyamines in rapid growth and cancer. Biochim Biophys Acta 473:241–293
Kobayashi Y, Kupelian J, Maudsley DV (1971) Ornithine decarboxylase stimulation in rat ovary by luteinizing hormone. Science 172:379–380
Persson L (1982) Antibodies to ornithine decarboxylase: Immunochemical cross-reactivity. Acta Chem Scand (in press)
Persson L, Rosengren E, Sundler F (1982) Localization of ornithine decarboxylase by immunocytochemistry. Biochem Biophys Res Commun 104:1196–1201
Sternberger LA (1979) Immunocytochemistry, 2nd edn. John Wiley, New York
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Persson, L., Rosengren, E. & Sundler, F. Immunohistochemical localization of ornithine decarboxylase in the rat ovary. Histochemistry 75, 163–167 (1982). https://doi.org/10.1007/BF00496007
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00496007