Summary
Parenchymal activities (μmol·min−1·g liver−1) and distributions of mitochondrial succinate dehydrogenase, cytosolic phosphoenolpyruvate carboxykinase and microsomal glucose-6-phosphatase were studied in regenerating rat liver after two thirds partial hepatectomy.
-
1.
Succinate dehydrogenase activity remained constant with a slight and transient increase for a few hours after operation. The typical periportal localization was changed to an almost even distribution from 8 h to 7 days; it was fully restored after 14 days.
-
2.
Phosphoenolpyruvate carboxykinase activity was increased by 1.8 fold 24 h after surgery; it remained enhanced until about 72 h. The normal periportal to perivenous enzyme gradient was diminished or replaced by a homogeneous distribution between 8 h and 7 days; the zonal heterogeneity was regained after 14 days.
-
3.
Glucose-6-phosphatase activity remained constant after partial hepatectomy. The normal periportal maximum was lost between 4 h and 36 h; the activity became more equally distributed and was even shifted towards the perivenous zone. After 48 h the zonal distribution was reestablished.
The results indicate that after partial hepatectomy the gluconeogenic capacity of the liver remnant is increased and that this increase is accompanied by a loss of the normal heterogeneity which is typical for the glucostat function of the organ. They reveal in addition that the three enzymes, representing three different subcellular compartments, change their zonal heterogeneity individually rather than synchronously.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Andersen B, Nath A, Jungermann K (1982) Heterogeneous distribution of phosphoenolpyruvate carboxykinase in rat liver parenchyma, isolated and cultured hepatocytes. Eur J Cell Biol 28:47–53
Andersen B, Zicrz S, Jungermann K (1983) Perinatal development of the distribution of phosphoenolpyruvate carboxykinase and succinate dehydrogenase in rat liver parenchyma. Eur J Cell Biol 30:126–131
Brinkmann A, Katz N, Sasse D, Jungermann K (1978) Increase of the gluconeogenic and decrease of the glycolytic capacity of rat liver with a change of the metabolic zonation after partial hepatectomy. Hoppe Seyler's Z Physiol Chem 359:1561–1571
Fischer W, Ick M, Katz N (1982) Reciprocal distribution of hexokinase and glucokinase in the periportal and perivenous zone of the rat liver acinus. Hoppe Seyler's Z Physiol Chem 363:375–380
Grisham JW (1962) A morphologic study of deoxyribonucleic acid synthesis and cell proliferation in regenerating rat liver; autoradiography with thymidine — 3H. Cancer Res 22:842–849
Guder W, Schmidt U (1976) Liver cell heterogeneity: the distribution of pyruvate kinase and phosphoenolpyruvate carboxykinase in the liver lobule of fed and starved rats. Hoppe Seyler's Z Physiol Chem 357:1793–1800
Gumucio JJ, Miller DL (1981) Functional implications of liver cell heterogeneity. Gastroenterology 80:393–403
Higgins GM, Anderson RM (1931) Experimental pathology of the liver. Arch Pathol 12:186–202
Jungermann K, Sasse D (1978) Heterogeneity of liver parenchymal cells. Trends Biochem Sci 3:198–202
Jungermann K, Katz N (1982) Functional hepatocellular heterogeneity. Hepatology 2:385–395
Katz N (1979) Correlation between rates and enzyme levels of increased gluconeogenesis in rat liver and kidney after partial hepatectomy. Eur J Biochem 98:535–542
Katz N, Brinkmann A, Jungermann K (1979) Compensatory increase of the gluconeogenic capacity of rat kidney after partial hepatectomy. Hoppe Seyler's Z Physiol Chem 360:51–57
Katz N, Teutsch HF, Jungermann K, Sasse D (1976) Perinatal development of the metabolic zonation of hamster liver parenchyma. FEBS Lett 69:23–28
Katz N, Teutsch H, Jungermann K, Sasse D (1977b) Heterogeneous reciprocal localization of fructose-1,6-bisphosphatase and of glucokinase in microdissected periportal and perivenous rat liver tissue. FEBS Lett 83:272–276
Katz N, Teutsch H, Sasse D, Jungermann K (1977a) Heterogeneous distribution of glucose-6-phosphatase in periportal and perivenous rat liver tissue. FEBS Lett 76:226–230
Lojda Z, Gossrau R, Schiebler TH (1976) Enzym-histochemistry Methoden. Springer, Berlin Heidelberg New York, p 231
Lowry OH, Rosebrough NY, Farr AL, Randall RJ (1951) Protein measurement with the Folin-phenol reagent. J Biol Chem 193:265–275
Nuber R, Teutsch HF, Sasse D (1980) Metabolic zonation in thioacetamide-induced liver cirrhosis. Histochemistry 69:277–288
Pool CW, Diegenbach PC, Scholten G (1979) Quantitative succinate dehydrogenase histochemistry. Histochemistry 64:251–262
Rabes HM, Wirsching R, Tuczell HV, Iseler G (1976) Analysis of cell cycle compartments of hepatocytes after partial hepatectomy. Cell Tissue Kinet 9:517–532
Sasse D, Katz N, Jungermann K (1975) Functional heterogeneity of rat liver parenchyma and of isolated hepatocytes. FEBS Lett 57:83–88
Schmidt U, Schmid H, Guder W (1978) Liver cell heterogeneity. The distribution of fructose-bisphosphatase in fed and fasted rats and in man. Hoppe Seyler's Z Physiol Chem 359:193–198
Seubert W, Huth W (1965) On the mechanism of gluconeogenesis and its regulation. II. The mechanism of gluconcogenesis from pyruvate and fumarate. Biochem Z 343:176–191
Teutsch H (1978) Quantitative determination of G6Pase activity in histochemical defined zones of the liver acinus. Histochemistry 58:281–288
Wachstein M, Meisel E (1957) Histochemistry of hepatic phosphatases at a physiologic pH. Am J Clin Pathol 27:13–23
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Andersen, B., Zierz, S. & Jungermann, K. Alteration in zonation of succinate dehydrogenase, phosphoenolpyruvate carboxykinase and glucose-6-phosphatase in regenerating rat liver. Histochemistry 80, 97–101 (1984). https://doi.org/10.1007/BF00492778
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00492778