Summary
The activity of acid phosphatase in skeletal muscle fibres of the plantaris and soleus of normal and dystrophic male hamsters was quantified using a histochemical post-coupling semipermeable membrane technique. Althoug the absolute levels of activity were found to vary widely from one animal to another, the ratio of the mean activities in the two muscles in each animal was virtually constant. In normal muscles, the ratio was about 0.73 and in dystrophic muscles, about 0.77. The activity in plantaris muscle fibres was always significantly lower than that in the corresponding soleus fibres, and in normal fibres compared to dystrophic ones. Another difference was that in normal fibres the mean activity declined to a constant level in mature animals older than about 3 months. In contrast, the activity in dystrophic muscles appeared to fall exponentially throughout life. The functional significance of these findings is discussed.
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References
Baxter JH, Suelter CH (1983) Skeletal muscle lysosomes — comparison of lysosomes from normal and dystrophic avian muscle pectoralis muscle as a function of age. Muscle Nerve 6:187–194
Baxter JH, Suelter CH (1984) Multiple acid phosphatases in avian pectoral muscle. The postmicrosomal supernatant acid phosphatase is elevated in avian dystrophic muscle. Arch. Biochem Biophys 228:397–406
Campbell JB, Stoward PJ (1981) changes of acid phosphatase activity with age in normal and dystrophic skeletal muscle. A quantitative histochemical study using a post-coupling semipermeable membrane technique. Proc R Microsc Soc 16:247
Christie KN, Stoward PJ (1977) A cytochemical study of acid phosphatase in dystrophic hamster muscle. J Ultrastruct Res 58:219–234
Dobrota M, Burge MLE, Hinton RN (1979) Apparent heterogeneity of hepatic lysosomes due to membrane-bound acid phosphatase. Eur J Cell Biol 19:139–144
Dubowitz V, Brooke MH (1973) Muscle biopsy. A modern approach. WB Saunders, London Philadelphia
Homburger F, Baker JR, Nixon CW, Wilgram G (1962) New hereditary disease of Syrian hamsters. Primary, generalized polmyopathy and cardiac necrosis. Arch Intern Med 110:660–662
Homburger F, Baker JR, Nixon CW, Wilgram G, Harrop J (1965) The early histopathological lesion of muscular dystophy in the Syrian golden hamster. J Pathol Bacteriol 89:133–138
Homburger F, Nixon CW, Eppemberger M, Baker JR (1966) Hereditary myopathy in the Syrian hamster: studies on pathogenesis. Ann NY Acad Sci 138:14–29
Lewis SEM, Kelly FJ, Goldspink DF (1984) Pre- and post-natal growth and protein turnover in smooth muscle, heart and slow-and fast-twitch skeletal muscles of the rat. Biochem J 217:517–526
Lojda L, Gutmann E (1976) Histochemistry of some acid hydrolases in striated muscles of the rat. Histochemistry 49:337–342
Mayersbach H von, Schmier HG (1981) Chronological aspects in histochemistry. Gustav Fischer, Stuttgart New York
Meijer AEFH (1972) Semipermeable membranes for improving the demonstration of enzyme activities in tissue sections. I. Acid phosphatase. Histochemie 30:31–39
Meijer AEFH, Israel DE (1978a) Evaluation of histochemical observations of activity of acid hydrolases obtained with semipermeable membrane techniques: a combined histochemical and biochemical investigation. 1. The histochemical investigation. Histochemistry 57:9–22
Meijer AEFH, Israel DE (1978b) Evaluation of histochemical observations of activity of acid hydrolases obtained with semipermeable membrane techniques: a combined histochemical and biochemical investigation. 2. The biochemical investigation and comparison with the histochemical observations. Histochemistry 57:23–31
Meijer AEFH, Vloedman AHT (1984) Diazonium inactivation in simultaneous-coupling and product inhibition in post-coupling azotechniques for demonstrating activity of acid hydrolases. Histochemistry 80:187–192
Meijer AEFH, Israel DE, van der Loos C, Tigges AJ (1979) Evaluation of histochemical observations of activity of acid hydrolases obtained with semipermeable membrane techniques. 3. The substrate specificity of isoenzymes of acid phosphatase in m. gastrocnemius of rabbits. Histochemistry 60:145–153
Montgomery A, Park DC, Pennington RJ (1974) Peptide hydrolases and AMP deaminase in extensor digitorum longus and soleus muscles from genetically dystrophic hamsters. Comp Biochem Physiol B 49:387–392
Pennington RJT (1978) Aspects of the biochemistry of the muscular dystrophies. In: Lunt GG, Marchbanks RM (eds) The biochemistry of myasthenia gravis and muscular dystrophy. Academic Press, London, pp 225–237
Sprent P (1969) Models in regression. Methuen, London
Stoward PJ, Al-Sarraj B (1981a) Quantitative histochemical investigations of semipermeable membrane techniques for the assay of acid phosphatase in skeletal muscle. I. Meijer's method. Histochemistry 71:405–431
Stoward PJ, Al-Sarraj B (1981b) Quantitative histochemical investigations of semipermeable membrane techniques for the assay of acid phosphatase in skeletal muscle III. A modified simultaneous coupling technique. Histochemistry 71:599–607
Stoward PJ, Raoof AMS (1983) Enzyme patterns in normal and diseased tissues: explorations with quantitative enzyme histochemistry. J Histochem Cytochem 31:1065
Stoward PJ, Campbell JB, Al-Sarraj B (1982) Quantitative histochemical investigations of semipermeable membrane techniques for the assay of acid phosphatase in skeletal muscle. IV. A post-coupling technique. Histochemistry 74:367–377
Tenniswood MP, Abrahams P, Bird CE, Clark AF (1978) Effects of castration and androgen replacement on acid phosphatase activity in the adult rat prostate gland. J Endocrinol 77:301–308
Tenniswood MP, Abrahams PP, Bird CE, Clark AF (1982) Ageassociated changes in acid phosphatase characteristics in the rat ventral prostate and other organs. Arch Androl 9:283–291
Trout JJ, Stauber WT, Schottelius BA (1979a) Cytochemical observations of two acid phosphatase-reactive structures in anterior latissmus dorsi muscle of the chicken. Histochem J 11:223–230
Trout JJ, Stauber WT, Schottelius BA (1979b) A unique acid phosphatase location: the transverse tubule of avian fast muscle. Histochem J 11:417–423
Trout JJ, Stauber WT, Schottelius BA (1981) Further studies on a unique T-tubular acid phosphatase in avian skeletal muscle. Histochem J 13:445–452
Weinstock IM, Iodice AA (1969) Acid hydrolase activity in muscular dystrophy and denervation atrophy. In: Dingle JT, Fell HB (eds) Lysosomes in biology and medicine, vol I. North Holland, Amsterdam London, pp 450–468
White MG, Stoward PJ, Christie KN, Anderson JM (1985) Proteases in normal and diseased human skeletal muscle: a preliminary histochemical survey. Histochem J 17:819–832
Wildenthal K, Decker RS, Poole AR, Dingle JT (1977) Age-related alterations in cardiac lysosomes. J Mol Cell Cardiol 9:859–866
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In honour of Prof. P. van Duijn
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Campbell, J.B., Stoward, P.J. Acid phosphatase activity in soleus and plantaris muscle fibres of normal and dystrophic hamsters. Histochemistry 84, 580–585 (1986). https://doi.org/10.1007/BF00482995
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DOI: https://doi.org/10.1007/BF00482995