Summary
The measurement of time correlated intracellular mitochondrial staining with 3,3′-dipetyloxacarbocyanine [Di-O-C5(3)] appeared of interest to define the optimal staining conditions. Mitochondrial staining of lymphocytes, monocytes and granulocytes results in different fluorescence signals, related to the numbers of mitochondria, that are present in the cells of these various cell types. Alterations of Di-O-C(5)3 staining in a distinct cell type are due to changes in the physiological or functional state of the mitochondria. It appeared that such alterations occur in cells, which are cultured in the presence of cytosine arabinoside. The effect of cytotoxic drugs upon the mitochondrial membrane potential may be of relevance for the understanding of the mechanism of action, exerted by cytotoxic drugs upon cell biology.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Bernal S, Shapiro HM, Chen LB (1981) Measurement of mitochondrial membrane potential as an assay for cytotoxicity of anti-cancer drugs. Proc Am Assoc Cancer Res 22:68
Bernal SD, Shapiro HM, Chen LB (1982) Monitoring the effect of anticancer drugs on L1210 cells by a mitochondrial probe, rhodamine 123. Int J Cancer 30:219–224
Beumer T, Pennings A, Beck H, Wessels J, Haanen C, Herman C (1983) Continuous measurement and analysis of staining kinetics by flow cytometry. Cytometry 4:244–249
Beumer T, Crissman H, Wessels H, Haanen C,(1984a) Calculation of S-phase numbers of four major cell categories in human bone marrow from DNA-flow cytometry and counterflow centrifugation data. Cytometry 5:494–500
Beumer T, Lenssinck H, Pennings A, Haanen C (1984b) An easy-to-build timer for kinetic measurements in flow cytometry. Cytometry 5:648–651
Cohen LB, Salzberg BM (1978) Optical measurement of membrane potential. Rev Physiol Biochem Pharmacol 83:35–88
Cohen RL, Muirhead KA, Gill JE, Waggoner AS, Horan PK (1981) A cyanine dye distinguishes between cycling and non-cycling fibroblasts. Nature 290:593–595
Cozzarelli NR (1977) The mechanism of action of inhibitors of DNA synthesis. Annu Rev Biochem 46:641–668
Darzynkiewicz Z, Staiano-Coico L, Melamed MR (1981) Increased mitochondrial uptake of rhodamine 123 during lymphocyte stimulation. Proc Natl Acad USA 78:2383–2387
De Mulder PHM, Wessels JMC, Rosenbrand DA, Smeulders JBJM, Wagener DJTh, Haanen C (1981) Monocyte purification with counterflow centrifugation, monitored by continuous flow cytometry. J Immunol Methods 47:31–38
Drenthe-Schonk A, Vierwinden G, Linssen P, Wessels H, Haanen C (1986) In vitro studies on phosphorylation and dephosphorylation of cytosine arabinoside in human leukemic cells. Leukemia Res (in press)
Goormaghtigh E, Pollakis G, Ruysschaert JM (1983) Mitochondrial membrane modifications induced by adriamycin mediated electron transport. Biochem Pharmacol 32:889–893
Haanen C, Muus P, Raymakers R, Drenthe-Schonk A, Salden M, Wessels J (1985) Studies on the cytotoxicity of cytosine arabinoside. Sem Oncol 12 (Suppl 3):120–129
Johnson LV, Walsh ML, Bockus BJ, Chen LB (1981) Monitoring of relative mitochondrial membrane potential in living cells by fluorescence microscopy. J Cell Biol 88:526–535
Johnson LV, Summerhayes IC, Chen LB (1982) Decreased uptake and retention of rhodamin 123 by mitochondria in feline sarcoma virus-transformed mink cells. Cell 28:7–14
Kufe DW, Grillin JD, Spriggs DR (1985) Cellular and clinical pharmacology of low dose Ara-C. Semi Oncol 12 (Suppl 3):200–207
Luisi-Deluca C, Mitchell T, Spriggs D, et al. (1984) Induction of terminal differentiation in human K 562 erythroleukemia cells by arabinofuranosylcytosine. J Clin Invest 74:821–827
Martin JC, Schwartzendruber DE (1980) Time: a new parameter for kinetic measurement in flow cytometry. Science 207:199–201
Sims PJ, Waggoner AS, Wang C-H, Hoffman JF (1974) Studies on the mechanism by which cyanine dyes measure membrane potential in red blood cells and phosphatidylcholine vesicles. Biochemistry 13:3315–3330
Valeriote F (1982) Cellular aspects of the action of cytosine arabioside. Med Pediat Oncol (Suppl) 1:5–26
Visser JWM, Bot SJH, van der Engh G (1981) Characterization and enrichement of murine hematopoietic stemcells by fluorescence activated cell sorting. Exp Hematol 9:644–655
Waggoner AS (1979) The use of cyanine dyes for the determination of membrane potentials in cells, organelles and vesicles. Methods Enzymol 55:689–695
Walsh Kinally K, Tedeschi H, Maloff BL (1978) Use of dyes to estimate the electrical potential of the mitochondrial membrane. Biochemistry 17:3419–3428
Author information
Authors and Affiliations
Additional information
In honour of Prof. P. van Duijn
Rights and permissions
About this article
Cite this article
Haanen, C., Muus, P. & Pennings, A. The effect of cytosine arabinoside upon mitochondrial staining kinetics in human hematopoietic cells. Histochemistry 84, 609–613 (1986). https://doi.org/10.1007/BF00482999
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00482999