Skip to main content
SpringerLink
Log in

Immunohistochemical demonstration of fibre type-specific isozymes of cytochrome c oxidase in human skeletal muscle

  • Published:
Histochemistry Aims and scope Submit manuscript

Summary

The immunohistochemical reaction of monoclonal as well as polyclonal antibodies against cytochrome c oxidase (COX) subunits with serial sections of normal human skeletal muscle was investigated. The stronger reactivity of polyclonal antibodies to COX subunits II–III and VIIbc with type I as compared to type II fibres, correlated well with the higher histochemical reactivity of NADH dehydrogenase, succinate dehydrogenase and cytochrome c oxidase in type I fibres. In contrast an almost exclusive reaction of a monoclonal antibody against subunit IV with type I fibre and a preponderan reaction of a polyclonal antibody against subunits Vab with type II fibres was obtained. Antibodies against subuntis I, Vb and VIc did not reveal a fibre-type-specific reactivity. The data indicate in human muscle the occurrence of fibre type-specific isozymes of cytochrome c oxidase differing in subunits IV and Va or Vb.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Büge, U, Kadenbach B (1986) Influence of buffer composition, membrane lipids and proteases on the kinetics of reconstituted cytochrome-c oxidase from bovine liver and heart. Eur. J. Biochem 161:383–390

    Article  PubMed  Google Scholar 

  • Brooke MH, Kaiser KK (1970) Three “myosin adenosine triphosphatase” systems: the nature of their pH lability and sulfhydryl dependence. J Histochem Cytochem 18:670–672

    PubMed  CAS  Google Scholar 

  • Chan SHP, Gai W-Z (1988) Monoclonal antibodies of mammalian cytochrome oxidase and its protein subunits. In: Kim CH, Tedeschi H, Diwan J-J, Salerno JC (eds) Advances in membrane biochemistry and bioenergetics. Plenum, New York, pp 193–202

    Google Scholar 

  • Dubowitz V, Brooke MH (1973) Muscle biopsy: a modern approach. Saunders, Philadelphia

    Google Scholar 

  • Greenberg BD, Newbold JE, Sugino A (1983) Intraspecific nucleotide sequence variability surrounding the origin of replication in human mitochondrial DNA. Gene:21:33–49

    Article  PubMed  CAS  Google Scholar 

  • Hood DA, Zak R, Pette D (1989) Chronic stimulation of rat skeletal muscle induces coordinate increases in mitochondrial and nuclear mRNAs of cytochrome-c-oxidase subunits. Eur J Biochem 179:275–280

    Article  PubMed  CAS  Google Scholar 

  • Johnson MA, Kadenbach B, Droste M, Old SL, Turnbull DM (1988) Immunocytochemical studies of cytochrome oxidase subunits in skeletal muscle of patients with partial cytochrome oxidase deficiencies. J Neurol Sci 87:75–90

    Article  PubMed  CAS  Google Scholar 

  • Kadenbach B, Hartmann R, Glanville R, Buse G (1982) Tissue-specific genes for polypeptide VIa of bovine liver and heart cytochrome c oxidase. FEBS Lett 138:236–238

    Article  PubMed  CAS  Google Scholar 

  • Kadenbach B (1983) Struktur und Evolution des Atmungsferments Cytochrom-c Oxidase (Structure and evolution of the “Atmungsferment” cytochrome-c oxidase). Angew Chem 95:273–281 (Angew Chem Int Edn Engl 22:275–282)

    Article  CAS  Google Scholar 

  • Kadenbach B, Jarausch J, Hartmann R, Merle P (1983) Separation of mammalian cytochrome c oxidase into 13 polypeptides by a sodium dodecyl sulfate-gel electrophoretic procedure. Anal Biochem 129:517–521

    Article  PubMed  CAS  Google Scholar 

  • Kadenbach B, Stroh A, Ungibauer M, Kuhn-Nentwig L, Büge U, Jarausch J (1986) Isozymes of cytochrome-c oxidase: characterization and isolation from different tissues. Methods Enzymol 126:32–46

    Article  PubMed  CAS  Google Scholar 

  • Kadenbach B, Kuhn-Nentwig L, Büge U (1987) Evolution of a regulatory enzyme: cytochrome-c oxidase (complex IV). Curr Top Bioenerg 15:113–161

    CAS  Google Scholar 

  • Kadenbach B, Hüther F-J, Büge U, Schlerf A, Johnson MA (1989) Regulatory complexity of cytochrome c oxidase and its defective manifestation in mitochondrial diseases. In: Azzi A, Drahota Z, Papa S (eds) Molecular basis of membrane-associated diseases. Springer, Berlin Heidelberg New York, pp 216–217

    Google Scholar 

  • Kadenbach B, Stroh A, Becker A, Eckerskorn C, Lottspeich F (1990) Tissue- and species specific expression of cytochrome c oxidase isozymes in vertebrates. Biochim Biophys Acta 1015:368–372

    Article  PubMed  CAS  Google Scholar 

  • Kuhn-Nentwig L, Kadenbach B (1985) Isolation and properties of cytochrome c oxidase from rat liver and quantification of immunological differences between isozymes from various rat tissues with subunit-specific antisera. Eur J Biochem 149:147–158

    Article  PubMed  CAS  Google Scholar 

  • Kuhn-Nentwig L, Kadenbach B (1986) Isolation and characterization of human heart cytochrome c oxidase. J Bioenerg Biomembr 18:307–314

    Article  PubMed  CAS  Google Scholar 

  • Merle P, Jarausch J, Trapp M, Scherka R, Kadenbach B (1981) Immunological and chemical characterization of rat liver cytochrome c oxidase. Biochim Biophys Acta 669:222–230

    PubMed  CAS  Google Scholar 

  • Monnat RJ, Loeb LA (1985) Nucleotide sequence preservation of human mitochondrial DNA. Proc Natl Acad Sci USA 82:2895–2899

    Article  PubMed  CAS  Google Scholar 

  • Nakagawa M, Miranda AF, Moggio M, Bonilla E, DiMauro S (1985) Demonstration of tissue-specific cytochrome-c-oxidase (COX) with monoclonal antibodies. Neurology 35 [Suppl 1]:95

    Google Scholar 

  • Nakagawa M, Miranda AF (1987) A monoclonal antibody against cytochrome c oxidase distinguishes cardiac and skeletal muscle mitochondria. Exp Cell Res 168:44–52

    Article  PubMed  CAS  Google Scholar 

  • Peters JH, Baumgarten H, Schulze M (1985) Monoklonale Antikörper. Herstellung und Charakterisierung. Springer, Berlin Heidelberg New York Tokyo

    Google Scholar 

  • Pette D, Urbova G (1985) Invited review: neural control of phenotypic expression in mammalian muscle fibres. Muscle Nerve 8:676–689

    Article  PubMed  CAS  Google Scholar 

  • Rizzuto R, Nakase H, Zeviani M, DiMauro S, Schon EA (1988) Subunit Va of human and bovine cytochrome c oxidase is highly conserved. Gene 69:245–256

    Article  PubMed  CAS  Google Scholar 

  • Schlerf A, Droste M, Winter M, Kadenbach B (1988) Characterization of two different genes (cDNA) for cytochrome c oxidase subunit VIa from heart and liver of the rat. EMBO J 7:2387–2391

    PubMed  CAS  Google Scholar 

  • Seligman AM, Karnousky MJ, Wasserkrug H, Hanker JS (1968) Non-droplet ultrastructural demonstration of cytochrome oxidase activity with a polymerizing osmiophilic reagent, diaminobenzidine (DAB). J Cell Biol 38:1–14

    Article  PubMed  CAS  Google Scholar 

  • Yanamura W, Zhang Y-Z, Takamiya S, Capaldi RA (1988) Tissue-specific differences between heart and liver cytochrome c oxidase. Biochemistry 27:4909–4914

    Article  PubMed  Google Scholar 

  • Zeviani M, Nakagawa M, Herbert J, Lomax MI, Grossman LI, Sherbany AA, Miranda AF, DiMauro S, Schon EA (1987) Isolation of a cDNA clone encoding subunit IV of human cytochrome c oxidase. Gene 55:205–217

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Unite de Recherche Biologie et Pathologie Neuromusculaires INSERM U 153, CNRS UA 614, 17 Rue du Fer-a-Moulin, F-75005, Paris, France

    N. Romero & M. Fardeau

  2. Faculte de Medecine Necker Enfants Malades, Laboratoire de Biochimie, INSERM U. 75, 156, Rue du Vaugirard, F-75730, Paris, Cedex 15, France

    C. Marsac

  3. Fachbereich Chemie, Biochemie, Philipps-Universität, Hans-Meerwein-Strasse, D-3550, Marburg, Federal Republic of Germany

    M. Droste, B. Schneyder & B. Kadenbach

Authors
  1. N. Romero
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. C. Marsac
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. M. Fardeau
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. M. Droste
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. B. Schneyder
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. B. Kadenbach
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Romero, N., Marsac, C., Fardeau, M. et al. Immunohistochemical demonstration of fibre type-specific isozymes of cytochrome c oxidase in human skeletal muscle. Histochemistry 94, 211–215 (1990). https://doi.org/10.1007/BF02440190

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02440190

Keywords

Search

Navigation