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Immunohistochemical demonstration of glycoconjugates bearing the type 2 chain-backbone structure in human fetal, normal and neoplastic gastrointestinal tract

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Summary

Immunohistochemical distributions of carbohydrate antigens based on the type 2 chain in normal as well as fetal and neoplastic tissues of human gastrointestinal tract were investigated with a monoclonal antibody (MAb) H11 (specific for type 2 chain) alone and in combination with the two MAbs MSG15 (for α2→6 sialylated type 2 chain) and IB9 (for the α2→6 sialylated type 2 chain and glycoproteins having NeuAcα2→6GalNAc), and 188C1 (for short- and long-chain Lex antigens) and FH2 (for the long-chain Lex antigen). In the pyloric mucosa of secretors, the type 2 chain is oncodevelopmentally expressed, but in non-secretors it is detected in surface mucous cells of normal gastric mucosa. The α2→6 sialylation, which is confined to endocrine cells of normal pyloric mucosa, occurs in fetal and carcinoma tissues. Irrespective of the secretor status, the short- and the long-chain Lex antigens can be detected in mature and immature glandular mucous cells of normal gastric mucosa, respectively; both antigens are also expressed in fetal and carcinoma tissues. In the colon, the type 2 chain and its α2→6 sialylated counterpart are expressed in an oncodevelopmental manner. The short- and the long-chain Lex antigens are significantly enhanced in colonic carcinoma. The glycoproteins with NeuAcα2→6GalNAc residues appear in gastric and colonic carcinoma as well as intestinalized gastric mucosa and transitional mucosa. Thus, some of these antigens were distinctively expressed in certain epithelial cells lining the normal gastrointestinal tract depending on maturation and patients' secretor status, and some were oncodevelopmental or carcinoma-associated antigens of the human gastrointestinal tract.

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Abbreviations

Fuc:

fucose

Gal:

galactose

GalNAc:

N-acetylgalactosamine

Glc:

glucose

GlcNAc:

N-acetylglucosamine

MAb:

monoclonal antibody

NeuAc:

N-acetylneuraminic acid

PBS:

phosphate-buffered saline

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Author notes

  1. Y. Hirabayashi

    Present address: Laboratory for Glyco Cell Biology, Division of Frontier Research Program, Riken (The Institute of Physical and Chemical Research), Hirokawa 2-1, 350-01, Wako, Japan

Authors and Affiliations

  1. Central Clinical Laboratories, Shinshu University Hospital, Asahi 3-1-1, 390, Matsumoto, Japan

    J. Nakayama, H. Ota, T. Katsuyama & M. Kanai

  2. Department of Biochemistry, Faculty of Medicine, Tokyo Medical and Dental University, Yushima 1-5-45, Bunkyo-ku, 113, Tokyo, Japan

    T. Taki

  3. Department of Biochemistry, University of Shizuoka, School of Pharmaceutical Science, Yada 395, 422, Shizuoka, Japan

    Y. Hirabayashi & Y. Suzuki

Authors
  1. J. Nakayama
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  2. H. Ota
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  3. T. Katsuyama
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  4. M. Kanai
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  5. T. Taki
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  6. Y. Hirabayashi
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  7. Y. Suzuki
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Nakayama, J., Ota, H., Katsuyama, T. et al. Immunohistochemical demonstration of glycoconjugates bearing the type 2 chain-backbone structure in human fetal, normal and neoplastic gastrointestinal tract. Histochemistry 97, 303–310 (1992). https://doi.org/10.1007/BF00270030

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