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Ischämie/Reperfusionsschaden der Leber durch freie radikale — direkter radikalnachweis mittels elektronenspinresonanz (ESR)

Free radicals in ischemia-reperfusia injury assessed by electron-spin-resonance (ESR)

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Summary

The generation of free oxygen radicals is presumed to be a pathogenetic principle in various conditions, primarily in postischemic reperfusion injury. Their assessment is difficult. ESR is an excellent tool to assess free radicals directly. 1n an experimental model of rat liver ischemia and reperfusion the increased generation of free radicals during reperfusion in liver tissue could be demonstrated after 60 min of liver ischemia. Elevated production rates of radicals could be detected after 5 min of reperfusion for at least 45 min with a maximum after 15 min of reperfusion. Scavenging of these radicals has to start in the very beginning of reperfusion.

Zusammenfassung

Freie Sauerstoffradikale werden als Kausalfaktor in einer Vielzahl von Erkrankungen, u. a. auch im postischämischen Reperfusionsschaden vermutet. Der Nachweis freier Radikale ist schwierig and wird in der Regel indirekt geführt. Mit der Elektronenspinresonanz gelingt es, in einem Tiermodell mit Leberischämie und -reperfusion das Auftreten von Radikalen im Lebergewebe nach 60minütiger Leberischämie nachzuweisen. Bereits nach 5minütiger Reperfusion treten vermehrte Radikale auf. Das Maximum der Radikalentstehung wird nach 15minütiger Reperfusion erreicht, wobei die übermäβige Produktion von freien Radikalen für mindestens 45 min Reperfusion nachweisbar bleibt. Soll these Kausalkette des postischämischen Reperfusionsschadens unterbrochen werden, müssen Fängermecha-nismen bereits zu Beginn der Reperfusion in ausreichender Konzentration im Gewebe vorhanden sein.

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Authors and Affiliations

  1. Abteilung für Allgemeine Chirurgie, Klinikum der Universität Ulm, Germany

    R. Kunz, M. Marzinzig & H. G. Beger (Direktor: Prof. Dr.)

  2. Abteilung für Organische Chemie I, Universität Ulm, Steinhövelstraβe 9, W-7900, Ulm, Bundesrepublik Deutschland

    H. A. Brune (Direktor: Prof. Dr.) & U. Ziegler

Authors
  1. R. Kunz
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  2. H. A. Brune
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  3. U. Ziegler
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  4. M. Marzinzig
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  5. H. G. Beger
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Kunz, R., Brune, H.A., Ziegler, U. et al. Ischämie/Reperfusionsschaden der Leber durch freie radikale — direkter radikalnachweis mittels elektronenspinresonanz (ESR). Langenbecks Arch Chir 376, 139–142 (1991). https://doi.org/10.1007/BF00250337

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  • DOI: https://doi.org/10.1007/BF00250337

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