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Wachstum und Synchronisierung der Alge Bumilleriopsis filiformis vischer (Xanthophyceae)

Growth and synchronization of cell division in the alga Bumilleriopsis filiformis

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Summary

  1. 1.

    Axenic cultures of Bumilleriopsis were cultivated in mineral medium under continuous illumination and diluted every two days. The growth has been calculated to be 0.50 log10/day units at the optimum temperature of 23 to 24° C.

  2. 2.

    In liquid medium one cell normally divides into 2n daughter cells (=aplanospores). Under our culture conditions generally 8 and 16 daughter cells are produced by one Bumilleriopsis cell within 48 h.

  3. 3.

    Bumilleriopsis filiformis is the first Xanthophycean species to be synchronized. Light-dark changes induce some synchronization, but a high percentage of simultaneous division and rapid completion of a cell division burst is brought about most effectively by cycles composed of strong light/dim light/temperature changes (LS-T). With the most effective combination tested, i.e. 33 h of white 15000 lux light followed by 15 h of white 3000 lux (dim) light at temperatures of 24° in the first and 19° C in the second light phase (=33:15 h/15000:3000 lux/24:19° C), 96% of the cells divide synchronously. The burst occurs in such a manner that, on an average, 50% of the cells have divided 38 h after the start of the cycle. These synchronizing conditions were maintained up to 30 cycles without interruption (=continuous synchronization).

  4. 4.

    Cell wall formation is observed (by staining mother cells with methylene blue) 1 to 2 h before the cells divide completely and their daughter cells are released.

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Abbreviations

EDTA:

Äthylendiamintetraessigsäure Dinatriumsalz

E 1000 nm :

Extinktion bei 1000 nm als Maß für die optische Dichte einer Suspension

LD:

Licht-Dunkel-Wechsel

LD-T:

Licht-Dunkel-Temperatur-Wechsel

LS-T:

Licht-Schwachlicht-Temperatur-Wechsel, wobei bei einem Regime x 1:x 2/y 1:y 2/z 1:z 2 die Indices die beiden Phasen desZyklus angeben, welche jeweils x h dauern und die Beleuchtungsstärke y Lux und die Temperatur z °C aufweisen; der Zyklus beginnt zur 0. Stunde der Phase x 1

Literatur

  • Belcher, J. H., Miller, J. D. A.: Studies on the growth of Xanthophyceae in pure culture IV. Nutritional types amongst the Xanthophyceae. Arch. Mikrobiol. 36, 219–228 (1960)

    Google Scholar 

  • Böger, P.: Das Strukturproteid aus Chloroplasten einzelliger Grünalgen und seine Beziehung zum Chlorophyll. Flora 154, 174–211 (1964)

    Google Scholar 

  • Böger, P.: Photophosphorylierung mit Chloroplasten aus Bumilleriopsis filiformis Vischer. Z. Pflanzenphysiol. 61, 85–97 (1969)

    Google Scholar 

  • Böger, P.: Algal preparations with photophosphorylation activity. In: Methods in enzymology, vol. XXIII, pt. A, p. 242–248, San Pietro, A., ed. London-New York: Acad. Press 1971

    Google Scholar 

  • Böger, P., Kiermayer, O.: Electron microscopy of plastids of Bumilleriopsis filiformis. Arch. Microbiol. 98, 207–214 (1974)

    Google Scholar 

  • Casselton, P. J.: Bumilleriopsis as a genus of the Xanthophyceae. Brit. Phycol. Bull. 2, 261–263 (1963)

    Google Scholar 

  • Fott, B.: Algenkunde. Jena: Fischer 1971

    Google Scholar 

  • Hoogenhout, H., Amesz, J.: Growth rates of photosynthetic microorganisms in laboratory cultures. Arch. Mikrobiol. 50, 10–25 (1965)

    Google Scholar 

  • Hřib, J., Nováček, M.: Beitrag zum Studium der Ontogenese der Alge Scenedesmus quadricauda (Turp.) Bréb. mittels der Methode der Zeitraffermikrokinematographie mit genauer Zeitaufzeichnung. Mikroskopie 29, 279–291 (1973)

    Google Scholar 

  • Komárek, J., Růžička, J., Simmer, J.: Synchronization of the cultures of Scenedesmus quadricauda by optimalizing the length of the light period. Biol. Plant. (Praha) 10, 177–189 (1968)

    Google Scholar 

  • Lorenzen, H., Hesse, M.: Synchronous cultures. In: Algal physiology and biochemistry, p. 894–908, Stewart, W. D. P., ed. London-Edinburg: Blackwell Publ.'s 1974 (im Druck)

    Google Scholar 

  • Lorenzen, H., Schleif, J.: Zur Bedeutung der kürzest möglichen Generationsdauer in Synchronkulturen von Chlorella. Flora, Abt. A, 156, 673–683 (1966)

    Google Scholar 

  • Lorenzen, H., Venkataraman, G. S.: Synchronous cell divisions in Anacystis nidulans Richter. Arch. Mikrobiol. 67, 251–255 (1969)

    PubMed  Google Scholar 

  • Miller, J. D. A., Fogg, G. E.: Studies on the growth of Xanthophyceae in pure cultures. I. The mineral nutrition of Monodus subterraneus Petersen. Arch. Mikrobiol. 28, 1–17 (1957)

    Google Scholar 

  • Möller, M., Senger, H.: Photosyntheseleistung synchroner Kulturen von Cyanidium caldarium. Ber. dtsch. bot. Ges. 85, 391–400 (1972)

    Google Scholar 

  • Padilla, G. M., James, T. W.: Synchronization of cell division in Astasia longa on a chemically defined medium. Exp. Cell. Res. 20, 401–415 (1960)

    Google Scholar 

  • Pecora, R. A., Rhodes, R. G.: A comparative study of growth in Bumilleriopsis filiformis. B. peterseniana, and Pseudobumilleriopsis pyrenoidosa (Xanthophyceae). Canad. J. Bot. 50, 337–341 (1972)

    Google Scholar 

  • Pirson, A., Lorenzen, H.: Ein endogener Zeitfaktor bei der Teilung von Chlorella. Z. Bot. 46, 63–67 (1958)

    Google Scholar 

  • Pirson, A., Lorenzen, H.: Synchronized dividing algae. Ann. Rev. Plant Physiol. 17, 439–459 (1966)

    Google Scholar 

  • Pogo, A. O., Arce, A.: Synchronization of cell division in Euglena gracilis by heat shock. Exp. Cell Res. 36, 390–397 (1964)

    Google Scholar 

  • Rooney, D. W., Yen, B.-C., Mikita, D. J.: Synchronization of Chlamydomonas division with intermittent hypothermia. Exp. Cell Res. 65, 94–98 (1971)

    Google Scholar 

  • Scherbaum, O., Zeuthen, E.: Induction of synchronous cell division in mass cultures of Tetrahymena piriformis. Exp. Cell Res. 6, 221–227 (1954)

    Google Scholar 

  • Senger, H.: Charakterisierung einer Synchronkultur von Scenedesmus obliquus, ihrer potentiellen Photosyntheseleistung und des Photosynthese-Quotienten während des Entwicklungscyclus. Planta (Berl.) 90, 243–266 (1970)

    Google Scholar 

  • Soeder, C. J.: Wirkungen von Außenfaktoren auf die Rhythmik der synchronen Zellvermehrung von Chlorella fusca Shihara et Krauss. Ber. dtsch. bot. Ges. 79, (138)-(146)(1966)

    Google Scholar 

  • Soeder, C. J.: Über den Zeitgeber der synchronen Zellentwicklung von Chlorella in Licht-Dunkel-Wechsel. Z. Pflanzenphysiol. 60, 5–11 (1968)

    Google Scholar 

  • Terry, O. W., Edmunds, L. N., Jr.: Phasing of cell-division by temperature cycles in Euglena cultured autotrophically under continuous illumination. Planta (Berl.) 93, 106–127 (1970)

    Google Scholar 

  • Vischer, W.: Heterokonten aus alpinen Böden, speziell dem schweizerischen Nationalpark. Ergebn. wiss. Untersuchg. Schweiz. Nationalparks I (neue Folge) Nr. 12, 481–512 (1945)

  • Wegmann, K., Metzner, H.: Synchronization of Dunaliella cultures. Arch. Mikrobiol. 78, 360–367 (1971)

    Google Scholar 

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  1. Fachbereich Biologie der Universität, Postfach 733, D-7750, Konstanz, Federal Republic of Germany

    Manfred Hesse

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  1. Manfred Hesse
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Hesse, M. Wachstum und Synchronisierung der Alge Bumilleriopsis filiformis vischer (Xanthophyceae). Planta 120, 135–146 (1974). https://doi.org/10.1007/BF00384923

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  • DOI: https://doi.org/10.1007/BF00384923

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