Skip to main content
SpringerLink
Log in

Photosynthetischer Elektronentransport der Alge Bumilleriopsis während der Zellentwicklung

Photosynthetic electron transport during cell development of the alga Bumilleriopsis filiformis

  • Published:
Planta Aims and scope Submit manuscript

Summary

The alga Bumilleriopsis filiformis Vischer (Xanthophyceae) was synchronized by light intensity combined with temperature changes. During the 48-h cell cycle there is a stage of low cellular photosynthetic activity in the 34th hour after start of the cycle and one of high activity between the 39th and 41th hour. These activities were compared with the p-benzoquinone mediated Hill reaction of non-homogenized cells and electron transport rates measured with carefully isolated chloroplast material. Ferricyanide and methylviologen reduction was tested with water as donor and photosystem I reactions with reduced dichloro-phenolindophenol and diaminodurene. The influence of superoxide dismutase and ascorbate was examined. The data show parallel changes in the activities of electron transport and cellular photosynthesis during cell development and indicate corresponding alteration not only in the activity of photosystem II but also in that of system I.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Abbreviations

pBQ:

p-Benzochinon

Chl:

Chlorophyll

DAD:

2,3,5,6-Tetramethyl-p-phenylendiamin (Diaminodurol)

DCMU:

3-(3′,4′-Dichlorphenyl)-1,1-dimethylharnstoff

DCIP:

2,6-Dichlorphenolindophenol

FCCP:

Carbonylcyanid-p-trifluormethoxyphenyl-hydrazon

FeCy:

Kaliumhexacyano-(III)-ferrat

LST:

(Stark)-Licht-Schwachlicht-Temperaturwechsel (zur Synchronisierung)

MV:

Methylviologen (1,1′-Dimethyl-4,4′-dipyridylium-dichlorid)

PS-I, PS-II:

Photosystem I bzw, II

SOD:

Superoxid-Dismutase

TRICIN:

N-Tris-(hydroxymethyl)-methylglycin (Puffer)

Literatur

  • Allen, J.F., Hall, D.O.: Superoxide reduction as a mechanism of ascorbate-stimulated oxygen uptake by isolated chloroplasts. Biochem. Biophys. Res. Commun. 52, 856–862 (1973)

    PubMed  Google Scholar 

  • Allen, J.F., Hall, D.O.: The relationship of oxygen uptake to electron transport in photosystem I of isolated chloroplasts: The role of superoxide and ascorbate. Biochem. Biophys. Res. Commun. 58, 579–585 (1974)

    PubMed  Google Scholar 

  • Asada, K., Urano, M., Takahashi, M.: Subcellular location of superoxide dismutase in spinach leaves and preparation and properties of crystalline spinach superoxide dismutase. Europ. J. Biochem. 36, 257–266 (1973)

    PubMed  Google Scholar 

  • Asada, K., Takahashi, M., Nagate, M.: Assay and inhibitors of spinach superoxide dismutase. Agric. Biol. Chem. 38, 471–473 (1974)

    Google Scholar 

  • Beauchamp, Ch., Fridovich, J.: Superoxide dismutase: improved assays and an assay applicable to acrylamide gels. Analyt. Biochem. 44, 276–287 (1971)

    PubMed  Google Scholar 

  • Bishop, N.I.: Mutations of unicellular green algae and their application to studies on the mechanism of photosynthesis. Rec. Chem. Progr. 25, 181–195 (1964)

    Google Scholar 

  • Böger, P.: Photophosphorylierung mit Chloroplasten aus Bumilleriopsis filiformis Vischer. Z. Pflanzenphysiol. 61, 85–97 (1969a)

    Google Scholar 

  • Böger, P.: Ferredoxin-katalysierte Reaktionen im zellfreien System der Alge Bumilleriopsis filiformis Vischer. Z. Pflanzenphysiol. 61, 447–461 (1969b)

    Google Scholar 

  • Böger, P., Schlue, U.: Long-term effects of herbicides on the photosynthetic apparatus. I. Influence of diuron, triazines, and pyridazinones. Weed Res. 16, 149–154 (1976)

    Google Scholar 

  • Elstner, E.F., Heupel, A., Vaklinova, S.: On the oxidation of hydroxylamine in isolated chloroplasts and the possible role of a peroxidase from spinach leaves in the oxidation of ascorbate and glycolate. Z. Pflanzenphysiol. 62, 184–200 (1970)

    Google Scholar 

  • Hauska, G., Trebst, A., Draber, W.: Lipophilicity and catalysis of photophosphorylation. II. Quinoid compounds as artificial carriers in cyclic photophosphorylation and photoreductions by photosystem I. Biochim. Biophys. Acta 305, 632–641 (1973)

    PubMed  Google Scholar 

  • Hesse, M.: Endogene Rhythmik der Produktionsfähigkeit bei Chlorella und ihre Beeinflussung durch Licht. Z. Pflanzenphysiol. 67, 58–77 (1972)

    Google Scholar 

  • Hesse, M.: Wachstum und Synchronisierung der Alge Bumilleriopsis filiformis Vischer (Xanthophyceae). Planta (Berl.) 120, 135–146 (1974a)

    Google Scholar 

  • Hesse, M.: Kern- und Chloroplastenteilung sowie Photosyntheseleistung synchron kultivierter Bumilleriopsis (Xanthophyceae). Ber. Dtsch. Bot. Ges. 87, 373–381 (1974b)

    Google Scholar 

  • Kok, B., Datko, E.A.: Reducing power generated in the second photoact of photosynthesis. Plant Physiol. 40, 1171–1177 (1965)

    PubMed  Google Scholar 

  • McCord, J.M., Fridovich, I.: Superoxide dismutase — an enzymic function for erythrocuprein (hemocuprein). J. Biol. Chem. 244, 6049–6055 (1969)

    Google Scholar 

  • Nelle, R., Tischner, R., Harnischfeger, G., Lorenzen, H.: Correlation between pigment systems and photosynthetic activity during the developmental cycle of Chlorella. Biochem. Physiol. Pflanz. (BPP) 167, 463–472 (1975)

    Google Scholar 

  • Schor, S., Siekevitz, P., Palade, G.E.: Cyclic changes in the thylakoid membranes of synchronized Chlamydomonas reinhardii. Proc. Nat. Acad. Sci. (Wash.) 66, 174–180 (1970)

    Google Scholar 

  • Senger, H.: Quantenausbeute und unterschiedliches Verhalten der beiden Photosysteme des Photosyntheseapparates während des Entwicklungsablaufs von Scenedesmus obliquus in Synchronkulturen. Planta (Berl.) 92, 327–346 (1970)

    Google Scholar 

  • Senger, H.: Changes in photosynthetic activities in synchronous cultures of Scenedesmus, Chlorella and Chlamydomonas. In: Colloques internationaux du C.N.R.S. No. 240. Les cycles cellulaires et leur blocage chez plusieurs protistes, pp. 101–108. Gif-sur-Yvette: C.N.R.S. 1974

    Google Scholar 

  • Senger, H., Bishop, N.I.: Changes in the photosynthetic apparatus during the life cycle of Scenedesmus obliquus. In: Progr. Photosynth. Res. Vol. I. pp. 425–434, Ed.: Metzner, H., Tübingen: Dr. Laupp 1969

    Google Scholar 

  • Senger, H., Frickel-Faulstich, B.: The regulation of electron flow in synchronized cultures of green algae. In: Proc. 3rd Int. Congr. Photosynth., Rehovot, pp. 715–727, Ed.: Avron, M. Amsterdam: Elsevier 1974

    Google Scholar 

  • Sorokin, C., Kruass, R.W.: Relative efficiency of photosynthesis in the course of cell development. Biochim. Biophys. Acta 48, 314–319 (1961)

    Article  Google Scholar 

  • Stein, J.R., ed.: Handbook of phycological methods. Cambridge: University Press 1973

    Google Scholar 

  • Tischner, R.: Physiologische Veränderungen synchroner Chlorella nach Hitzebehandlung unter besonderer Berücksichtigung der hitzeempfindlichsten Phase. Dissertation, Göttingen 1974

  • Werthmüller, K.: Photosynthetisches Verhalten von Synchronkulturen beim Übergang von Autotrophie zur Mixotrophie. Ber. Dtsch. Bot. Ges. 85, 435–442 (1972)

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Fachbereich Biologie der Universität Konstanz, Postfach 7733, D-7750, Konstanz, Federal Republic of Germany

    Manfred Hesse, Peter Bley & Peter Böger

Authors
  1. Manfred Hesse
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Peter Bley
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Peter Böger
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Hesse, M., Bley, P. & Böger, P. Photosynthetischer Elektronentransport der Alge Bumilleriopsis während der Zellentwicklung. Planta 132, 53–59 (1976). https://doi.org/10.1007/BF00390330

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00390330

Search

Navigation