Abstract
A crude organelle preparation from germinating castor bean endosperm catalysed the incorporation of galactose from UDP[14C]galactose into chloroform/methanol (2:1)-soluble glactolipids. At least two galactolipids were formed. Most of the [14C]galactose was present in a galactolipid synthesized by the microsomal membranes, the remainder was present in a second galactolipid synthesized by other cellular membranes, possibly Golgi-derived. The addition of asialo-agalacto-fetuin reduced incorporation of [14C]galactose into the microsomal galactolipid with a concomitant increase in microsomal [14C]galactoprotein. Asialo-agalacto-fetuin did not affect galactolipid or galactoprotein synthesis by nonmicrosomal fractions. The results suggest that the endoplasmic reticulum is a major site of protein galactosylation in castor bean endosperm cells, and that galactose transfer from UDP-galactose to protein occurs via a lipid-linked intermediate.
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Abbreviations
- ER:
-
endoplasmic reticulum
- ASGF:
-
asialoagalacto-fetuin
- IDPase:
-
inosine diphosphatase
- TCA:
-
trichloroacetic acid
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Mellor, R.B., Lord, J.M. Involvement of a lipid-linked intermediate in the transfer of galactose from UDP [14C]galactose to exogenous protein in castor bean endosperm homogenates. Planta 147, 89–96 (1979). https://doi.org/10.1007/BF00384596
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DOI: https://doi.org/10.1007/BF00384596