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Polyadenylated mRNA for the light-harvesting chlorophyll a/b protein

Its presence in green and absence in chloroplast-free plant cells

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Abstract

In thylakoid membranes isolated from green plants of parsley, pea, and barley, the light-harvesting chlorophyll a/b protein complex (LHCP, mol. weight: 25,000), is a major constituent. Poly(A)RNA isolated from these species was translated in a wheat germ, cell-free system. The in vitro translation products were treated with antibodies raised against the LHCP. This treatment resulted in the precipitation of a precursor protein (mol. weight: 29,000). Poly(A)RNA was also prepared from a cell culture ofPetroselinum that does not develop chloroplasts upon illumination. This poly(A)RNA is capable of stimulating amino acid incorporation in the in vitro translation system, however, it does not direct the synthesis of LHCP.

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Abbreviations

LHCP:

light-harvesting chlorophyll a/b protein

PAL:

phenylalanine ammonia-lyase

References

  • Apel, K. (1979) Phytochrome-induced appearance of mRNA activity for the apoprotein of the light-harvesting chlorophyll a/b protein of barley (Hordeum vulgare). Eur. J. Biochem.97, 183–188

    Article  CAS  PubMed  Google Scholar 

  • Apel, K., Kloppstech, K., (1978) The plastid membranes of barley (Hordeum vulgare). Light-induced appearance of the mRNA coding for the apoprotein of the light-harvesting chlorophyll a/b protein. Eur. J. Biochem.85, 581–588

    Article  CAS  PubMed  Google Scholar 

  • Armond, P.A., Staehelin, L.A., Arntzen, C.J. (1977) Spatial relationship of photosystem I, photosystem II and the light-harvesting complex in chloroplast membranes. J. Cell Biol.73, 400–418

    Article  CAS  PubMed  Google Scholar 

  • Bogorad, L. (1967) In: Organization biosynthesis, pp. 395–418, Vogel, A.J., Lampen, J.O., Bryson, V., eds., Academic Press, New York

    Google Scholar 

  • Bonner, W.M., Laskey, R.A. (1974) A film detection method for tritium-labelled proteins and nucleic acids in polyacrylamide gels. Eur. J. Biochem.46, 83–88

    Article  CAS  PubMed  Google Scholar 

  • Chua, N.H., Blomberg, F. (1979) Immunochemical studies of thylakoid membrane polypeptides from spinach andChlamydomonas reinhardtii. J. Biol. Chem.254, 215–223

    CAS  PubMed  Google Scholar 

  • Chua, N.H., Matlin, K., Bennoun, P. (1975) A chlorophyll-protein complex lacking in photosystem I mutants ofChlamydomonas reinhardtii. J. Cell Biol.67, 361–377

    Article  CAS  PubMed  Google Scholar 

  • Kloppstech, K., Schweiger, H.G. (1976) In vitro translation of poly(A)RNA fromAcetabularia. Cytobiologie13, 394–400

    CAS  Google Scholar 

  • Meyer, T.S., Lamberts, B.L. (1965) Use of Coomassie brilliant blue R 250 for the electrophoresis of microgram quantities of parotid saliva proteins on acrylamide-gel strips. Biochim. Biophys. Acta107, 144–145

    CAS  PubMed  Google Scholar 

  • Müller, M., Viro, M., Balke Ch., Kloppstech, K. (1979) Light-induced appearance of the rRNA for a chloroplast membrane protein in polysomes. Its in vitro translation and processing of the precursor protein. Hoppe Seyler's Z. Physiol. Chem.360, 330

    Google Scholar 

  • Neville, D.M. (1971) Molecular weight determination of proteindodecylsulfate complexes by gel electrophoresis in a discontinous buffer system. J. Biol. Chem.246, 6328–6334

    CAS  PubMed  Google Scholar 

  • Roberts, B.E., Paterson, B.M. (1970) Efficient translation of tobacco mosaic virus RNA and globin 9 S RNA in a cell-free system from commercial wheat germ. Proc. Natl. Acad. Sci. USA70, 2330–2334

    Google Scholar 

  • Schröder, J., Kreuzaler, F., Schäfer, E., Hahlbrock, K. (1979) Concomitant induction of phenylalanine ammonia-lyase and flavanone synthase mRNAs in irradiated plant cells. J. Biol. Chem.254, 57–65

    PubMed  Google Scholar 

  • Seitz, U., Richter, G. (1970) Isolierung und Charakterisierung schnell markierter, hochmolekularer RNS aus frei suspendierten Calluszellen der Petersilie (Petroselinum sativum). Planta92, 309–326

    Article  CAS  Google Scholar 

  • Spieß, E., Seitz, U. (1975) Quantitative determination of growth-dependent changes in the ultrastructure of freely suspended callus cells of parsley (Petroselium crispum). Ber. Dtsch Bot. Ges.88, 319–328

    Google Scholar 

  • Takebe, J., Labib, G., Melchers, G. (1971) Regeneration of whole plants from isolated mesophyll protoplasts from tobacco. Naturwissenschaften58, 318–320

    Article  Google Scholar 

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Authors and Affiliations

  1. Institut für Botanik, Universität Hannover, Herrenhäuser Straße 2, D-3000, Hannover 21, Federal Republic of Germany

    Michael Müller, Maija Viro, Christiane Balke & Klaus Kloppstech

Authors
  1. Michael Müller
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  2. Maija Viro
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  3. Christiane Balke
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  4. Klaus Kloppstech
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Müller, M., Viro, M., Balke, C. et al. Polyadenylated mRNA for the light-harvesting chlorophyll a/b protein. Planta 148, 444–447 (1980). https://doi.org/10.1007/BF02395312

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  • DOI: https://doi.org/10.1007/BF02395312

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