Abstract
Protoplasts prepared by enzymic treatment of protonemata of the moss Physcomitrella patens regenerate rapidly in white light (15 W m−2). The great majority of protoplasts follow a simple regenerative sequence, namely: cell wall synthesis; formation of an asymmetric cell; division of the asymmetric cell, and further extension and division to produce a new chloronemal filament. Only cell wall formation occurs independently of light. The production of an asymmetric cell requires relatively high photon fluence rates of blue or red light and ceases upon transfer to darkness. The subsequent stages of regeneration require much lower photon fluence rates, and red light is considerably more effective than blue or far-red light in permitting cell division. This system is of interest in the study of the induction of cell polarity in plants.
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References
Ashton, N.W., Cove, D.J. (1977) The isolation and preliminary characterisation of auxotrophic and analogue resistant mutants of the moss Physcomitrella patens. Mol. Gen. Genet. 154, 87–95
Ashton, N.W., Grimsley, N.H., Cove, D.J. (1979) Analysis of gametophytic development in the moss Physcomitrella patens using auxin and cytokinin resistant mutants. Planta 144, 427–435
Batra, A., Abel, W.O. (1981) Development of moss plants from isolated and regenerated protoplasts. Plant Sci. Lett. 20, 183–189
Binding, H. (1966) Regeneration und Verschmelzung nackter Laubmoosprotoplasten. Z. Pflanzenphysiol. 55, 305–321
Bopp, M., Zimmermann, S., Knoop, B. (1980) Regeneration of protonema with multiple DNA content from isolated protoplasts of the moss Funaria hygrometrica. Protoplasma 104, 119–127
Burgess, J., Linstead, P.J. (1981) Studies on the growth and development of protoplasts of the moss Physcomitrella patens, and its control by light. Planta 151, 331–338
Cove, D.J., Ashton, N.W., Featherstone, D.R., Wang, T.L. (1980) The use of mutant strains in the study of hormone action and metabolism in the moss Physcomitrella patens. In: Proc. 4th John Innes Symp., pp. 231–241, Davies, D.R., Hopwood, D.A., eds. John Innes Charity, Norwich
Cove, D.J., Schild, A., Ashton, N.W., Hartmann, E. (1978) Genetic and physiological studies of the effect of light on the development of the moss Physcomitrella patens. Photochem. Photobiol. 27, 249–254
Gay, L. (1976) The development of leafy gametophytes from isolated protoplasts of Polytrichum juniperinum Willd. Z. Pflanzenphysiol. 79, 33–39
Grimsley, N.H., Ashton, N.W., Cove, D.J. (1977) The production of somatic hybrids by protoplast fusion in the moss Physcomitrella patens. Mol. Gen. Genet. 154, 97–100
Gwozdz, E.A., Walisweska, B. (1979) Regeneration of enzymatically isolated protoplasts of the moss Funaria hygrometrica (L.) Sibth. Plant Sci. Lett. 15, 41–45
Hughes, J., McCully, M.E. (1975) The use of an optical brightener in the study of plant structure. Stain Technol. 50, 319–329
Jaffe, L.F. (1968) Localisation in the developing Fucus egg and the general role of localising currents. Adv. Morphog. 7, 295–328
Maeda, M. (1979) Isolation and culture of protoplasts from moss protonemata. Bot. Mag. 92, 105–110
Quatrano, R.S. (1978) Development of cell polarity. Annu. Rev. Plant Physiol. 29, 487–510
Saxena, P.K., Rashid, A. (1980) Development of gametophores from isolated protoplasts of the moss Anoectangium thomsonii Mitt. Protoplasma 103, 401–406
Stadleman, D.J., Kinzel, H. (1972) Vital staining of plant cells. In: Methods in cell physiology, vol. 5, pp. 326–327, Prescott, D.M., ed. Academic Press, London New York
Stumm, I., Meyer, Y., Abel, W.O. (1975) Regeneration of the moss Physcomitrella patens (Hedw.) from isolated protoplasts. Plant Sci. Lett. 5, 113–118
Weisenseel, M.H. (1979) Induction of polarity. In: Encyclopedia of plant physiology, N.S., vol. 7: Physiology of movements, pp. 485–505, Haupt, W., Feinleib, M.E., eds. Springer, Berlin Heidelberg New York
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Jenkins, G.I., Cove, D.J. Light requirements for regeneration of protoplasts of the moss Physcomitrella patens . Planta 157, 39–45 (1983). https://doi.org/10.1007/BF00394538
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DOI: https://doi.org/10.1007/BF00394538