Abstract
Immuno-gold labelling using a monoclonal antibody (PCBC3) with a primary specificity for α-L-arabinofuranosyl residues was used to locate these residues in pollen tubes of Nicotiana alata grown in vivo. The antibody bound to the outer fibrillar layer of the pollen-tube wall: the inner, non-fibrillar wall layer was not labelled. Cytoplasmic vesicles (0.2 μm diameter) were also labelled. The antibody may bind to an arabinan in the pollen-tube wall.
Similar content being viewed by others
References
Anderson, M.A., Sandrin, M.S., Clarke, A.E. (1984) A high proportion of hybridomas raised to a plant extract secrete antibody to arabinose or galactose. Plant Physiol. 75, 1013–1016
Bacic, A., Harris, P.J., Stone, B.A. (1987) Structure and function of plant cell walls. In: The biochemistry of plants, vol. 12: Carbohydrates, Preiss, J. ed., Academic Press, New York (in press)
Clarke, A.E., Anderson, M.A., Bacic, A., Harris, P.J., Mau, S.-L. (1985) Molecular basis of cell recognition during fertilization in higher plants. J. Cell Sci. Suppl. 2, 261–285
de Nettancourt, D., Devreux, M., Bozzini, A., Cresti, M., Pacici, E., Sarfatti, G. (1973) Ultrastructural aspects of the self-incompatibility mechanism in Lycopersicum peruvianum Mill. J. Cell Sci. 12, 403–419
Engels, F.M. (1974) Function of Golgi vesicles in relation to cell wall synthesis in germinating Petunia pollen. II. Chemical composition of Golgi vesicles and pollen tube wall. Acta Bot. Neerl. 23, 81–89
Fincher, G.B., Stone, B.A. (1981) Metabolism of noncellulosic polysaccharides. In: Encyclopedia of plant physiology, N.S. vol. 13B: Plant carbohydrates II: Extracellular carbohydrates, pp. 68–132, Tanner, W., Loewus, F.A., eds. Springer-Verlag, Berlin Heidelberg New York
Gell, A.C. (1985) Arabinogalactan-proteins of the female sexual tissue of Nicotiana alata. M.Sc. thesis, University of Melbourne, Vic., Australia
Harris, P.J., Anderson, M.A., Bacic, A., Clarke, A.E. (1984) Cell-cell recognition in plants with special reference to the pollen stigma interaction. Oxford Surveys Plant Molec. Cell Biol. 1, 161–203
Harris, P.J., Freed, K., Anderson, M.A. Weinhandl, J.A., Clarke, A.E. (1987) An enzyme-linked immunosorbent assay (ELISA) for in vitro pollen growth based on binding of a monoclonal antibody to the pollen-tube surface. Plant Physiol. (in press)
Herrero, M., Dickinson, H.G. (1981) Pollen tube development in Petunia hybrida following compatible and incompatible intraspecific matings. J. Cell Sci. 47, 365–383
Kroh, M., Knuiman, B. (1982) Ultrastructure of cell wall and plugs of tobacco pollen tubes after chemical extraction of polysaccharides. Planta 154, 241–250
Lawton, J.R., Harris, P.J. (1978) Fixation of senescing plant tissues: sclerenchymatous fibre cells from the flowering stem of a grass. J. Microsc. 112, 307–318
Rae, A.L., Harris, P.J., Bacic, A., Clarke, A.E. (1985) Composition of the cell walls of Nicotiana alata Link et Otto pollen tubes. Planta 166, 128–133
Roberts, K., Phillips, J., Shaw, P., Grief, C., Smith, E. (1985) An immunological approach to the plant cell wall. In: Biochemistry of plant cell walls, pp. 125–154, Brett, C.T., Hillman, J.R., eds. Cambridge University Press, Cambridge, UK
Ruel, K., Joseleau, J.-P. (1984) Use of enzyme-gold complexes for the ultrastructural localization of hemicelluloses in the plant cell wall. Histochemistry 81, 573–580
Salema, R., Brandào, O.I. (1973) The use of Pipes buffer in the fixation of plant cells for electron microscopy. J. Submicrosc. Cytol. 5, 79–96
Sedgley, M., Blesing, M.A., Bonig, I., Anderson, M.A., Clarke, A.E. (1985) Arabinogalactan-proteins are localized extracellularly in the transmitting tissue of Nicotiana alata Link et Otto, an ornamental tobacco. Micro Microsc. Acta 16, 247–254
Spurr, A.R. (1969) A low viscosity resin embedding medium for electron microscopy. J. Ultrastruct. Res. 26, 31–65
Stone, B.A., Evans, N.A., Bonig, I., Clarke, A.E. (1984) The application of Sirofluor, a chemically defined fluorochrome from aniline blue for the histochemical detection of callose. Protoplasma 122, 191–195
Thiéry, J.P. (1967) Mise en évidence des polysaccharides sur coupes fines en microscopie électronique. J. Micros. 6, 987–1018
van der Woude, W.J., Morré, D.J., Bracker, C.F. (1971) Isolation and characterisation of secretory vesicles in germinated pollen of Lilium longiflorum. J. Cell Sci. 8, 331–351
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Anderson, M.A., Harris, P.J., Bonig, I. et al. Immuno-gold localization of α-L-arabinofuranosyl residues in pollen tubes of Nicotiana alata Link et otto. Planta 171, 438–442 (1987). https://doi.org/10.1007/BF00392290
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00392290