Abstract
Diamine-oxidase (DAO; EC 1.4.3.6) activity and di-and polyamine levels were estimated along the epicotyl and root of light-grown and etiolated lentil (Lens culinaris Medicus) and pea (Pisum sativum L.) seedlings. The activity of DAO was higher in etiolated epicotyls than in lightgrown ones. In both species there was a positive correlation between DAO activity and the diamine (putrescine and cadaverine) levels along the whole epicotyl and root. Polyamine (spermine and spermidine) distribution seemed to be associated with the meristematic and elongating zone of the epicotyl and root. The physiological function of DAO is discussed in relation to its possible role in providing hydrogen peroxide to peroxidase-dependent reactions occurring in the cell wall.
Similar content being viewed by others
Abbreviations
- CAD:
-
cadaverine
- DA:
-
diamine
- DAO:
-
diamine oxidase
- PA:
-
polyamine
- PUT:
-
putrescine
- SPD:
-
spermidine
- SPM:
-
spermine
References
Angelini R., Di Lisi, F., Federico, R. (1985) Immunoaffinity purification and characterization of diamine oxidase from Cicer. Phytochemistry 24, 2511–2513
Angelini, R., Federico, R. (1986) Occurrence of diamine oxidase in the apoplast of Leguminosae seedlings. In: Biomedical studies of natural polyamines, pp 183–189 Caldarera, C.M., Clò, C., Guarnieri, C. eds. CLUEB Bologna, Italy
Arrigoni, O. (1982) Rapportí tra acido ascorbico e meccanismi biologici di difesa. G. Bot. Ital. 116, Suppl 1, 3–5
Bagni, N., Serafini-Fracassini, D., Torrigiani, P. (1982) Polyamines and cellular growth processes in higher plants. In: Plant growth substances 1982, pp. 473–482, Wareing, P.F., ed. Academic Press, London New York
Dumortier, F.M., Flores, H.E., Shekhawat, N.S., Galston, A.W. (1983) Gradients of polyamines and their biosynthetic enzymes in coleoptiles and roots of corn. Plant Physiol. 72, 915–918
Federico, R., Angelini, R. (1986) Occurrence of diamine oxidase in the apoplast of pea epicotyls. Planta 167, 300–302
Federico, R., Angelini, R., Cesta, A., Pini, C. (1985a) Determination of diamine oxidase in lentil seedlings by enzymic activity and immunoreactivity. Plant Physiol 79, 62–64
Federico, R., Angelini, R., Argento-Ceru, M.P., Manes, F. (1985b) Immunohistochemical demonstration of lentil diamine oxidase. Cell. Mol. Biol. 31, 171–174
Flores, H.E., Filner, P. (1985) Polyamine catabolism in higher plants: Characterization of pyrroline dehydrogenase. Plant Growth Regul. 3, 277–291
Goldberg, R., Perdrizet, E. (1984) Ratio of free to bound polyamines during maturation in mung-bean hypocotyl cells. Planta 161, 531–535
Goren, R., Palavan, N., Flores, H.E., Galston, A.W. (1982) Changes of polyamine titer in etiolated pea seedlings following red light treatments. Plant Cell Physiol 23, 19–26
Kaur-Sawhney, R., Flores, H.E., Galston, A.W. (1981) Polyamine oxidase in oat leaves: a cell wall-localized enzyme. Plant Physiol. 68, 494–498
Lin, P.P.C. (1984) Polyamine anabolism in germinating Glycine max (L.) seeds. Plant Physiol 76, 372–380
Macholàn, L., Minář, J. (1974) The depression of pea diamine oxidase due to light and the verification of its participation in growth processes using competitive inhibitors,. Biol. Plant. 16, 86–93
Shen, H., Galston, A.W. (1985) Correlation between polyamine ratios and growth patterns in seedling roots. Plant Growth Regul. 3, 353–363
Smith, T.A. (1985) Polyamines. Annu. Rev. Plant Physiol. 36, 117–143
Werle, E., Beaucamp G., Schirren, V. (1959) Über die Aminoxydase von Erbsenkeimlingen und ihre Bedeutung für die Keimung. Planta 53, 125–133
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Federico, R., Angelini, R. Distribution of polyamines and their related catabolic enzyme in etiolated and light-grown leguminosae seedlings. Planta 173, 317–321 (1988). https://doi.org/10.1007/BF00401018
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00401018