Skip to main content
Log in

The identification and quantitative analysis of abscisic acid in plant extracts by gas-liquid chromatography

  • Published:
Planta Aims and scope Submit manuscript

Summary

New techniques are described which permit the quantitative analysis of microgram quantities of abscisic acid in plant extracts by gas chromatography. Presumptive methyl abscisate peaks on gas chromatograms are positively identified by photosensitised isomerisation to methyl 2-trans-abscisate. Losses of abscisic acid during pre-purification are corrected by using 2-trans-abscisic acid as an internal standard.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  • Bennet-Clark, T. A., Kefford, N. P.: Chromatography of the growth substances in plant extracts. Nature (Lond.) 171, 645–647 (1953).

    Google Scholar 

  • Davis, L. A., Heinz, D. E., Addicott, F. T.: Gas-liquid chromatography of trimethylsilyl derivatives of abscisic acid and other plant hormones. Plant Physiol. 43, 1389–1394 (1968).

    Google Scholar 

  • Eagles, C. F., Wareing, P. F.: Experimental induction of dormancy in Betula pubescens. Nature (Lond.) 199, 874–875 (1963).

    Google Scholar 

  • —: The role of growth substances in the regulation of bud dormancy. Physiol. Plantarum (Cph.) 17, 697–708 (1964).

    Google Scholar 

  • Gaskin, P., MacMillan, J.: Plant hormones. VII. Identification and estimation of abscisic acid in a crude plant extract by combined gas chromatography-mass spectrometry. Phytochem. 7, 1699–1701 (1968).

    Google Scholar 

  • Lenton, J. R., Bowen, M. R., Saunders, P. F.: Detection of abscisic acid in the xylem sap of willop (Salix viminalis, L.) by gas-liquid chromatography. Nature (Lond.) 220, 86–87 (1968).

    Google Scholar 

  • Milborrow, B. V.: The identification of (+) abscisin II in plants and measurement of its concentrations. Planta (Berl.) 76, 93–113 (1967).

    Google Scholar 

  • Mousseron-Canet, M., Mani, J. C., Dalle, J. P., Olive, J. L.: Photoxydation sensibilisée de quelques composés apparantes à la dehydro-β-ionone, synthèse de l'ester methylique de la (±) abscisine. Bull. Soc. chim. Fr. 12, 3874 (1966).

    Google Scholar 

  • Phillips, I. D. J., Wareing, P. F.: Effect of photoperiod on the level of growth inhibitors in Acer pseudoplatanus. Naturwissenschaften 13, 317 (1958a).

    Google Scholar 

  • —: Studies in the dormancy of sycamore. I. Seasonal changes in growth substance content of the shoot. J. exp. Bot. 9, 350–364 (1958b).

    Google Scholar 

  • —: Studies in the dormancy of sycamore. II. The effect of daylength on the natural growth inhibitor content of the shoot. J. exp. Bot. 10, 504–514 (1959).

    Google Scholar 

  • Robinson, P. M., Wareing, P. F.: Chemical nature and biological properties of the inhibitor varying with photoperiod in sycamore (Acer pseudoplatanus). Physiol. Plantarum (Cph.) 17, 314–323 (1964).

    Google Scholar 

  • Schlenk, H., Gellerman, J. L.: Esterification of fatty acids with diazomethane on a small scale. Analyt. Chem. 32, 1412–1414 (1960).

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Lenton, J.R., Perry, V.M. & Saunders, P.F. The identification and quantitative analysis of abscisic acid in plant extracts by gas-liquid chromatography. Planta 96, 271–280 (1971). https://doi.org/10.1007/BF00386942

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00386942

Keywords

Navigation