Summary
65 cases of focal lymphocytic thyroiditis and Hashimoto's disease and five cases of thyroiditis de Quervain were studied with immunohistological methods. In both focal lymphocytic thyroiditis and Hashimoto's disease, lymph follicles with active germinal centers were found which contained germinal center cells that stained positively for intracytoplasmic immunoglobulins (heavy and/or light chains). Positively staining germinal center cells made up only a minor portion of overall immunoglobulin-positive cells; most of the positive infiltrating cells were plasmacytes arranged in small groups or clusters among thyroid follicles. Thus the number of immunoglobulin-containing cells differed greatly between focal lymphocytic thyroiditis, where sites of infiltration were represented by lymph follicles, and Hashimoto's disease. In the former, only a few cells outside lymph follicles stained positively for intracytoplasmic immunoglobulins, whereas in the latter numerous cells within areas of coherent infiltration did. Furthermore, in most cases of Hashimoto's disease macrophages and giant cells with positive staining for lysozyme were present in variable numbers, while in focal thyroiditis they were less frequent or absent. Between these two immunohistologically separable groups, i.e. focal lymphocytic thyroiditis and Hashimoto's disease, there were many cases with features of both. Considering the occurrence of such intermediate forms and some immunohistological similarities between Hashimoto's disease and focal lymphocytic thyroiditis (nearly identical ratio of the different immunoglobulin classes and similar distribution of immunoglobulin-positive germinal center cells), it is likely that these lesions represent different activities of a same immunological process.
Thyroiditis de Quervain was characterized immunologically by numerous macrophage clusters and giant cells that both stained positively for lysozyme. Compared with the giant cells seen in Hashimoto's disease (mainly of Langhans type), those of de Quervain's thyroiditis (mainly of foreign body type) were larger and more numerous. Lymph follicles (with or without active germinal centers) were not observed. Among infiltrating cells, numerous plasmacytes that stained positively for intracytoplasmic immunoglobulins were identified. Their number and the distribution pattern of the different classes of immunoglobulins contained within them was similar to those seen in Hashimoto's disease.
Similar content being viewed by others
Literature
Balfour BM, Doniach D, Roitt IM, Couchman KG (1961) Fluorescent antibody studies in human thyroiditis: Auto-antibodies to an antigen of the thyroid colloid distinct from thyroglobulin. Br J Exp Pathol 42:307–16
Batolo D, Martines F, Carrozza G (1967) Morphogénèse, histochimie et signification des structures gigantocellulaires dans les thyroïdites idiopathiques. Pathol Eur 3:80–95
Blizzard RM, Hamwi GJ, Skillman TG, Wheeler WE (1959) Thyroglobulin antibodies in multiple thyroid diseases. N Engl J Med 260:112–6
Cline MJ, Selenkow HA, Brooke MS (1959) Autoimmunity in thyroid disease. N Engl J Med 260:117–21
Doniach D, Roitt IM (1957) Auto-immunity in Hashimoto's disease and its implications. J Clin Endorinol 17:1293–1304
Eden KC, Trotter WR (1941/42) A case of lymphadenoid goitre associated with the full clinical picture of Graves' disease. Br J Surg 29:320–2
Eylan E, Zmucky R (1957) Mumps virus and subacute thyroiditis. Evidence of a causal association. Lancet I:1062–3
Fagraeus A, Jonsson J (1970) Distribution of organ antigens over the surface of thyroid cells as examined by the immunofluorescence test. Immunology 18:413–6
Fatourechi V, McConahey WM, Woolner LB (1971) Hyperthyroidism associated with histologic Hashimoto's thyroiditis. Proc Mayo Clin 46:682–9
Felix-Davies D (1958) Autoimmunisation in subacute thyroiditis associated with evidence of infection by mumps virus. Lancet I:880–3
Furr WE, Crile G jr (1954) Struma lymphomatosa: clinical manifestations and response to therapy. J Clin Endocrinol 14:79–86
Gleichmann E, Deicher H (1968) Quantitative Immunglobulin-Bestimmungen im Serum bei entzündlichen Leberkrankheiten. II. Chronisch entzündliche Lebererkrankungen. Klin Wschr 46:801–2
Glynne A, Thomson JA (1972) Serum immunoglobulin levels in thyroid disease. Clin Exp Immunol 12:71–8
Grobler P, Buerki H, Cottier H, Hess MW, Stoner RD (1974) Cellular bases for relative radioresistance of the antibody-forming system at advanced stages of the secondary response to tetanus toxoid in mice. J Immunol 112:2154–65
Holborow EJ, Brown PC, Roitt IM, Doniach D (1959) Cytoplasmic localization of “complement-fixing” auto-antigen in human thyroid epithelium. Br J Exp Pathol 40:583–9
Khalid BAK, Hamilton NT, Cauchi MN (1976) Binding of thyroid microsomes by lymphocytes from patients with thyroid disease and normal subjects. Clin Exp Immunol 23:28–32
Kidd A, Okita N, Row VV, Volpé R (1980) Immunologic aspects of Graves' and Hashimoto's diseases. Metabolism 29:80–99
Laryea E, Row VV, Volpé R (1973) The effect of blood leucocytes from patients with Hashimoto's disease on human thyroid cells in monolayer culture. Clin Endocrinol 2:23–35
Lennert K, Caesar R, Müller HK (1967) Electron microscopic studies of germinal centers in man. In: Cottier H, Odartchenko N, Schindler R (ed) Germinal Centers in Immune Response. Springer, Berlin Heidelberg New York, pp 49–59
Lindsay S, Dailey ME, Friedlander J, Yee G, Soley MH (1952) Chronic thyroiditis: A clinical and pathologic study of 354 patients. J Clin Endocrinol 12:1578–1600
Lobo A, Carr I, Malcolm D (1978) The EM immunocytochemical demonstration of lysozyme in macrophage giant cells in sarcoidosis. Experientia 34:1088–9
Maagøe H, Reintoft I, Christensen HE, Simonsen J, Mogensen EF (1977) Lymphocytic thyroiditis. I. Correlation between morphological, immunological and clinical findings. Acta Med Scand 201:299–302
McGregor A, McLachlan S, Clark F, Rees Smith B, Hall R (1979) Thyroglobulin and microsomal autoantibody production by cultures of Hashimoto peripheral blood lymphocytes. Immunology 36:81–5
McLachlan SM, McGregor A, Rees Smith B, Hall R (1979) Thyroid autoantibody synthesis by Hashimoto thyroid lymphocytes. Lancet I:162–3
Mellors RC, Brzosko WJ, Sonkin LS (1962) Immunopathology of chronic nonspecific thyroiditis (autoimmune thyroiditis). Am J Pathol 41:425–36
Nève P (1970) Ultrastructure of the thyroid in de Quervain's subacute granulomatous thyroiditis. Virchows Arch [Pathol Anat] 351:87–98
Perrudet-Badoux A, Frei PC (1969) On the mechanism of “rosette” formation in human and experimental thyroiditis. Clin Exp Immunol 5:117–27
Podleski WK (1971) Quantitative distribution of IgG, IgM and IgA immunoglobulins in lymphocytic thyroiditis of the Hashimoto type. Arch Immunol Ter dosw 19:431–8
Porter DD, Fennell RH (1961) Serologic and immunohistochemical study of human and experimental thyroiditis. N Engl J Med 265:830–4
Reidbord HE, Fisher ER (1973) Ultrastructural features of subacute granulomatous thyroiditis and Hashimoto's disease. Am J Clin Pathol 59:327–37
Richter E, Wick G, Zambelis N, Ludwig H, Schernthaner G (1978) Demonstration and characterization of thyroglobulin-binding peripheral blood cells in Hashimoto patients by fluoroimmunocytoadherence. Clin Immunol Immunopathol 11:178–89
Roberts IM, Whittingham S, Mackay IR (1973) Tolerance to an autoantigen-thyroglobulin. Antigen-binding lymphocytes in thymus and blood in health and autoimmune disease. Lancet II:936–40
Roitt IM, Doniach D (1958) Human auto-immune thyroiditis: serological studies. Lancet II:1027–33
Roitt IM, Doniach D, Campbell PN, Vaughan Hudson R (1956) Auto-antibodies in Hashimoto's disease (lymphadenoid goitre). Lancet II:820–1
Satoh M (1976) Ultrastructure of the giant cell in de Quervain's subacute thyroiditis. Acta Pathol Jap 26:133–7
Schwarze EW, Papadimitriou C (1977) Maligne Lymphome der Schilddrüse. Verh Dtsch Ges Pathol 61:328–35
Sternberger LA, Hardy PH, Cuculis JJ, Meyer HG (1970) The unlabeled antibody enzyme method of immunohistochemistry. Preparation and properties of soluble antigen-antibody complex (horseradish peroxidase-antihorseradish peroxidase) and its use in identification of spirochetes. J Histochem Cytochem 18:315–33
Stuart AE, Allan WSA (1958) Auto-antibodies in thyroid carcinoma. Lancet II:47
Terashima K, Imai Y, Kasajima T, Tsunoda R, Takahashi K, Kojima M (1977) An ultrastructural study on antibody production of the lymph nodes of rats with special reference to the role of germinal centers. Acta Pathol Jpn 27:1–24
Tomasi TB, Tisdale WA (1964) Serum gamma-globulins in acute and chronic liver diseases. Nature 201:834–5
Tötterman TH (1978) Distribution of T-, B-, and thyroglobulin-binding lymphocytes infiltrating the gland in Graves' disease, Hashimoto's thyroiditis, and de Quervain's thyroiditis. Clin Immunol Immunopathol 10:270–7
Tötterman TH, Andersson LC, Häyry P (1979) Evidence for thyroid antigen-reactive T lymphocytes infiltrating the thyroid gland in Graves' disease. Clin Endocrinol 11:59–68
Trotter WR, Belyavin G, Waddams A (1957) Precipitating and complement-fixing antibodies in Hashimoto's disease. Proc Roy Soc Med 50:961–2
Tsunoda R, Terashima K, Takahashi K, Kojima M (1978) An ultrastructural study with enzyme-labeled antibody technique on immunoglobulin-containing cells in human tonsils, especially in germinal centers. Acta Pathol Jpn 28:53–75
Urbaniak SJ, Penhale WJ, Irvine WJ (1973) Circulating lymphocyte subpopulations in Hashimoto thyroiditis. Clin Exp Immunol 15:345–54
Vickery AL, Hamlin E (1961) Struma lymphomatosa (Hashimoto's thyroiditis). Observations on repeated biopsies in sixteen patients. N Engl J Med 264:226–9
Wick G, Richter E, Zambelis N, Ludwig H, Schernthaner G (1977) Demonstration of thyroglobulin-binding cells in Hashimoto patients by fluoroimmunocytoadherence. Lancet II:1180
Woolner LB, McConahey WM, Beahrs OH (1959) Struma lymphomatosa (Hashimoto's thyroiditis) and related thyroidal disorders. J Clin Endocinrol 19:53–83
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Knecht, H., Saremaslani, P. & Hedinger, C. Immunohistological findings in Hashimoto's thyroiditis, focal lymphocytic thyroiditis and Thyroiditis de Quervain. Virchows Arch. A Path. Anat. and Histol. 393, 215–231 (1981). https://doi.org/10.1007/BF00431078
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00431078