Skip to main content
SpringerLink
Log in

Immunohistochemical observations of keratins, involucrin, and epithelial membrane antigen in urinary bladder carcinomas from patients infected withSchistosoma haematobium

  • Published:
Virchows Archiv A Aims and scope Submit manuscript

Summary

Squamous cell carcinomas of the urinary bladder and the epithelial lesions associated with infection bySchistosoma haematobium were histopathologically and immunohistochemically described for keratin proteins (TK, 41–65 kDa; KL1, 55–57 kDa; PKK1, 40, 45 and 52.5 kDa), involucrin, and epithelial membrane antigen (EMA). Normal urothelial epithelium was positive for all keratins, and showed absent or slight reactions for involucrin and EMA in superficial umbrella cells. The intestinal type of epithelium was composed of columnar cells and small basal cells; TK was positive in the basal cells, KL1 staining was positive in the columnar cells, whereas PKK1 was negative or slight in the columnar cells. Involucrin was confined to columnar cells. Squamous metaplastic epithelium showed a rather regional keratin distribution: TK was distributed in all layers, KL1 decorated upper spinous and granular layers, but PKK1 did not bind, and involucrin staining existed only in upper spinous and granular cells. Keratin expression in squamous cell carcinomas indicated heterogeneity and its stainability was dependent on the degree of keratinization: The G 1 type revealed strong reaction, the G 2 type showed a similar distribution pattern, but the staining intensity was less, and the G3 type showed irregular staining with decreased intensity. Involucrin staining was limited to keratinized cells of carcinoma as was that for EMA.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Asamoto M, Fukushima S, Tatemoto Y, Mori M (1987) Immunohistochemical expression of keratin protein urinary bladder carcinoma. Urol Res (submitted)

  • Bilharz T (1852) Fernere Beobachtungen über das die Pfortader des Menschen bewohnende Distomum Haematobium und sein Verhältnis zu gewissen pathologischen Bildungen. Ztschr Wissenschaft Zool 4:72–76

    Google Scholar 

  • Brown RR, Kuntz RE, Arend RA, Moore JA, Huang TC (1976) Experimental Bilharzial Bladder Cancer: tryptphan metabolism in non-human primates experimentally infected withSchistosoma haematobium. J Natl Cancer Inst 56:101–104

    Google Scholar 

  • Cheever AW, Kuntz RE, Moore JA, Bryan GT, Brown RR (1976) Animal mode of human disease: Carcinoma of the urinary bladder inSchistosoma haematobium infection. Am J Pathol 84:673–676

    Google Scholar 

  • Cordell J, Richardson TC, Pulford KAF, Ghosh AK, Gatter KC, Heyderman E, Mason DY (1985) Production monoclonal antibodies against human epithelial membrane antigen for use in diagnostic immunocytochemistry. Br J Cancer 52:347–354

    Google Scholar 

  • Dimmette RM, Sproat HF, Sayegh ES (1956) The classification of carcinoma of the urinary bladder associated with schistosomiasis and metaplasia. J Urol 75:680–686

    Google Scholar 

  • El-Bolkainy MN, Eissa S, Mokhtar N (1981 a) Histopathology of the proliferative and metaplastic lesions. Ed. El-Bolkainy, Chu EW P84-105, A1-Ahram Press Cairo Egypt

    Google Scholar 

  • El-Bolkainy MN, Tawfik HN, Kamel IA (1981 b) Histopathologic classification of carcinomas in the Schistosomal bladder. Ed. El-Bolkainy, Chu EW P106-123, A1-Ahhram Press Cairo Egypt

    Google Scholar 

  • Friedman NB, Ash JE (1959) Tumors of the urinary bladder. Atlas of tumor pathology, Section VIII-Fascicle, 31/a AFIP Washington DC

    Google Scholar 

  • Gillman J, Prates MD (1962) Histological types and histogenesis of bladder cancer in the portuguese east african with special reference to Bilharzial cystitis. Acta Un Int Cancer 18:560–574

    Google Scholar 

  • Goebel C (1905) Über die bei Bilharziakrankheit vorkommenden Blasentumoren mit besonderer Berücksichtigung des Carcinoms. Ztschr Krebsforsch 3:369–513

    Google Scholar 

  • Gusterson BA, Lucas RB, Ormerod MG (1982) Distribution of epithelial membrane antigen in benign and malignant lesions of the salivary glands. Virchows Arch A (Pathol Anat) 397:227–233

    Google Scholar 

  • Hashem M (1961) The aetiology and pathogenesis of the bilharzial bladder cancer. J Egypt Med Ass 44:857–966

    Google Scholar 

  • Heyderman E, Strudly I, Powell G, Richardson TC, Cordell JL, Mason DY (1985) A new monoclonal antibody to epithelial membrane antigen(EMA)-E29. A comparison of its immunocytochemical reactivity with polyclonal anti-EMA antibodies and with another monoclonal antibody, HMFG-2. Br J Cancer 52:355–361

    Google Scholar 

  • Higashi GI, Aboul-Enein MI (1981) Diagnosis and epidemiology ofSchistosoma haematobium infections in Egypt. Ed. El-Bolkainy, Chu EW P54-69, Al-Ahram Press Cairo Egypt

    Google Scholar 

  • Higginson J, Oettle AG (1962) Cancer of the bladder in the south african bantu. Acta Un Int Cancer 18:579–584

    Google Scholar 

  • Itoiz ME, Conti CJ, Gimenez IB, Lanfranchi HE, Fernandez-Alonso GI, Klein-Szanto AJP (1986) Immunodetection of involucrin in lesion of the oral mucosa. J Oral Pathol 15:205–208

    Google Scholar 

  • Khalafalla AS, Abul-Fadl MA (1964) Studies on the urinary excretion of certain tryptophan metabolites before and after tryptophan loading dose in bilharziasis, bilaharzial bladder cancer and certain other types of malignancies in Egypt. Br J Cancer 13:592–604

    Google Scholar 

  • Koss LG (1975) Tumors of the urinary bladder. Atlas of tumor pathology. Second series Fascicle II AFIP Washington DC

    Google Scholar 

  • Koss LG (1985) Tumors of the urinary bladder. Atlas of tumor pathology. Supplement Fascicle II (second series) AFIP Washington DC

    Google Scholar 

  • Kuntz RE, Cheever AW, Myers BJ (1972) Proliferative epithelial lesions of the urinary bladder of Nonhuman Primates infected withSchistosoma haematobium. J Natl Cancer Inst 48:223–235

    Google Scholar 

  • Löenig T, Straquet M-T, Thivolet J, Seifert G (1980) Keratin polypeptides distribution in normal and diseased human epidermis and oral mucosa. Immunohistochemical study on unaltered epithelium and inflammatory, premalignant and malignant lesions. Virchows Arch A (Pathol Anat) 388:273–288

    Google Scholar 

  • Löenig T, Staquest M-J, Schmitt D, Thivolet J (1982) Immunocytochemical and ultrastructural localization of keratin polypeptides in normal epidermis and mucosal cells and tissues. J Invest Dermatol 78:44–47

    Google Scholar 

  • Moll R, Franke WW, Schiller DL, Geiger B, Krepler R (1982) The catalogue of human cytokeratins: Patterns of expression in normal epithelia, tumors and cultured cells. Cell 31:11–24

    Google Scholar 

  • Moore DV, Meleney HE (1954) Comparative susceptibility of common laboratory animals to experimental infection withSchistosoma haematobium. J Parasitol 40:392–397

    Google Scholar 

  • Mori M, Nakai M, Hyung K-H, Noda Y, Kawamura K (1985) Distribution of keratin proteins in neoplastic and tumor-like lesions of squamous epithelium. Oral Surg 59:63–69

    Google Scholar 

  • Mustacchi P, Shimkin MB (1966) Cancer of the bladder and infestation withSchistosoma heamatobium. J Natl Cancer Inst 20:825–842

    Google Scholar 

  • Nakai M, Mori M (1986) Immunohistochemical distribution of monoclonal antibodies against keratin in papillomas and carcinomas from oral and nasopharyngeal regions. Oral Surg 62:292–302

    Google Scholar 

  • Nelson WG, Sun T-T (1983) The 50- and 58-kdalton keratin classes as molecular markers for stratified squamous epithelia: Cell culture research. J Cell Biol 97:244–251

    Google Scholar 

  • Pinkus GS, Kurtin PJ (1985) Epithelial membrane antigen — a diagnostic discriminant in surgical pathology. Immunohistochemical profile in epithelial, mesenchymal and hematopoietic neoplasms using paraffin sections and monoclonal antibodies. Hum Pathol 16:929–940

    Google Scholar 

  • Pinkus GS, Etheridge CL, O'Conner EM (1986) Are keratin proteins a better tumor marker than epithelial membrane antigen? A comparative immunohistochemical study of various paraffin-embedded neoplasms using monoclonal and monoclonal antibodies. Am J Clin Pathol 85:269–277

    Google Scholar 

  • Said JW, Sasson AF, Shintaku IP, Banks-Schlegel S (1984) Involucrin in squamous and basal cell carcinomas of the skin: An immunohistochemical study. J Invest Dermatol 82:449–452

    Google Scholar 

  • Sumitomo S, Kumasa S, Iwai Y, Mori M (1986) Involucrin expression in epithelial tumors of oral and pharyngeal mucosa and skin. Oral Surg 62:155–163

    Google Scholar 

  • Sun T-T, Eichner R, Nelson WG, Tseng SCG, Weiss RA, Jarvinen M, Woodcock-Mitchell J (1983) Keratin classes: Molecular markers for different types of epithelial differentiation. J Invest Dermatol 81:109–115

    Google Scholar 

  • Tatemoto Y, Kumasa S, Watanabe Y, Mori M (1987a) Immunohistochemical expression of monoclonal antibody against epithelial membrane antigen in salivary gland tumors. Acta histochem cytochem (in press)

  • Tatemoto Y, Saka M, Tanimura T, Mori M (1987b) Immunohistochemical observation on binding of monoclonal antibody to epithelial membrane antigen in epithelial tumors of the oral activity and skin. Oral Surg (in press)

  • Thomas P, Said JW, Nash GD, Banks-Schlegel S (1984) Profiles of keratin proteins in basal and squamous cell carcinoma of the skin. An immunohistochemical study. Lab Invest 50:36–41

    Google Scholar 

  • Tubura A, Morii S, Hilkens J, Hilgers J (1985) Expression of MAM-3 and MAM-6 antigens in endometorial and endocervical adenocarcinomas. Virchows Arch A (Pathol Anat) 407:59–67

    Google Scholar 

  • Viac J, Reano A, Thivolet J (1982) Cytokeratins in human basal and squamous cell carcinomas: Biochemical immunohistological findings and comparisons with normal epithelia. J Cutan Pathol 9:377–390

    Google Scholar 

  • Viac J, Reano A, Brochier J, Staquet M-J, Thivolet J (1983) Reactivity pattern of a monoclonal antikeratin antibody (KL1). J Invest Dermatol 81:351–354

    Google Scholar 

  • Walts AE, Said JW, Siegel MB, Banks-Schlegel S (1985) Involucrin, a marker of squamous and urothelial differentiation. An immunohistochemical study on its distribution in normal and neoplastic tissues. J Pathol 145:329–340

    Google Scholar 

  • Warhol MJ, Antonioli DA, Pinkus GS, Burke L, Rice RH (1982) Immunoperoxidase staining for involucrin, a potential diagnostic aid in cervicovaginal pathology. Hum Pathol 13:1095–1099

    Google Scholar 

  • Woodcock-Mitchell J, Eichner R, Nelson WG, Sun T-T (1982) Immunolocalization keratin polypeptides in human epidermis using monoclonal antibodies. J Cell Biol 95:580–588

    Google Scholar 

  • Zotter S, Lossnitzer A, Kunze K-D, Müller M, Hilkens J, Hilgers J, Hageman P (1985) Epithelial markers for paraffin-embedded human tissues. Immunohistochemistry with monoclonal antibodies against milk fat globule antigens. Virchows Arch A (Pathol Anat) 406:237–251

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. First Department of Pathology, Nagoya City University Medical School, Mizuho-cho, Mizuho-Ku, 467, Nagoya, Japan

    Shoji Fukushima & Nobuyuki Ito

  2. Department of Pathology, National Cancer Institute, Cairo, Egypt

    Mohamed N. El-Bolkainy & Hassan Nabil Tawfik

  3. Department of Oral and Maxillofacial Surgery, Asahi University School of Dentistry, Hozumi-cho, Motosu-gun, 501-02, Gifu, Japan

    Yukihiro Tatemoto & Masahiko Mori

Authors
  1. Shoji Fukushima
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Nobuyuki Ito
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Mohamed N. El-Bolkainy
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Hassan Nabil Tawfik
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Yukihiro Tatemoto
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Masahiko Mori
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Fukushima, S., Ito, N., El-Bolkainy, M.N. et al. Immunohistochemical observations of keratins, involucrin, and epithelial membrane antigen in urinary bladder carcinomas from patients infected withSchistosoma haematobium . Vichows Archiv A Pathol Anat 411, 103–115 (1987). https://doi.org/10.1007/BF00712734

Download citation

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00712734

Key words

Search

Navigation