Abstract
The expression of involucrin, a structural component of the envelope of mature squamous epithelium, was studied in 166 paraffin-embedded breast carcinomas. In 41 cases (24.7%) involucrin-positive, light microscopically non squamous tumour cells were detected. The number of involucrin-positive tumour cells varied considerably from case to case. For further characterization, involucrin-positive cases were studied using monoclonal antibodies to various cytokeratins (PKK1, EAB 903, EAB 904) and, in selected cases, double immunostaining with antibodies to cytokeratins and involucrin were performed. Coexpression of involucrin and cytokeratins demonstrated by PKK1 was seen in all tumour cells, whereas coexpression of involucrin and cytokeratins detected by EAB 904 was only seen in single and scattered cells in a few cases. Cytokeratins detected by EAB 903 were not coexpressed with involucrin in our cases. Our results indicate heterogeneity of cytokeratins in breast carcinomas and suggest a dissociation in the regulation of involucrin and cytokeratin expression.
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Cordell JL, Falini B, Erber WN, Gosh AK, Abulaziz Z, Mac Donald S, Pulford K, Stein H, Mason D (1984) Immuno-enzymatic labelling of monoclonal antibodies using immune complexes of alkaline phosphatase and monoclonal anti-alkaline phosphatase (APAAP complexes). J Histochem Cytochem 32:219–229
Elsayed A, Richart RM, Crum CP (1987) Involucrin expression in cervical intraepithelial neoplasia: a critical evaluation. Gynecol Oncol 26:25–34
Gown AM, Vogel AM (1982) Monoclonal antibodies to intermediate filament proteins of human cells: unique and cross-reacting antibodies. J Cell Biol 95:414–424
Gown AM, Vogel AM (1984) Monoclonal antibodies to human intermediate filament proteins. II. Distribution of filament proteins in normal human tissues. Am J Pathol 114:309–321
Green H (1979) The keratinocyte as a differentiated cell type. Harvey Lect 74:101–139
Itoiz ME, Conti CJ, Gimenez IB, et al. (1986) Immunodetection of involucrin in lesions of the oral mucosa. J Oral Pathol Med 15:205–208
Jarasch E-D, Nagle RB, Kaufmann M, Maurer C, Böcker WJ (1988) Differential diagnosis of benign epithelial proliferations and carcinomas of the breast using antibodies to cytokeratins. Hum Pathol 19:276–289
Kamino H, Huang S, Fu Y (1988) Keratin and involucrin immunohistochemistry of nasopharyngeal carcinoma. Cancer 61:1142–1148
Kanitakis J, Zambruno G, Viac J, Thivolet J (1986) Involucrin expression in adnexal skin tumours. An immunohistological study. Virchows Arch 408:527–540
Klein-Szanto AJ, Boysen M, Reith A (1987) Keratin and involucrin in preneoplastic and neoplastic lesions. Distribution in the nasal mucosa of nickel workers. Arch Pathol Lab Med 111:1057–1061
Mayall FG, Goddard H, Gibbs AR (1992) An assessment of involucrin as a diagnostically useful immunohistochemical marker in lung tumours. Histopathology 20:53–55
Michel S, Schmidt R, Robinson SM, Shroot B, Reichert U (1987) Identification and subcellular distribution of cornified envelope precursor proteins in the transformed human keratinocyte line SV-K14. J Invest Dermatol 88:301–305
Moll R, Franke WW, Schiller DL, Geiger B, Krepier R (1982) The catalog of human cytokeratins, patterns of expression in normal epithelial tumors and cultured cells. Cell 31:11–24
Murphy GF, Flynn TC, Rice RH, Pinkus GS (1984) Involucrin expression in normal and neoplastic human skin: a marker for keratinocyte differentiation. J Invest Dermatol 82:453–457
Rice RH, Green H (1979) Presence in human epidermal cells of a soluble protein precursor of the cross-linked envelope: activation of the cross-linking by calcium ions. Cell 18:681–694
Rice RH, Pinkus GS, Warhol MJ, Antonioli DA (1984) Involucrin: biochemistry and immunohistochemistry. In: DeLellis RA (ed) Advances in immunochemistry. Masson Publishing, New York, pp 111–125
Said JW, Nash G, Sassoon AF, Shintaku IP, Banks-Schlegel S (1983) Involucrin in lung tumors: a specific marker for squamous differentiation. Lab Invest 49:563–568
Said JW, Sassoon AF, Shintaku IP, Banks-Schlegel S (1984) Involucrin in squamous and basal cell carcinomas of the skin-an immunohistochemical study. J Invest Dermatol 82:449–452
Simon M, Green H (1984) Participation of membrane-associated proteins in the formation of the cross-linked envelope of the keratinocyte. Cell 36:827–834
Simon M, Green H (1985) Enzymatic cross-linking of involucrin and other proteins by keratinocyte particulates in vitro. Cell 40:677–683
Simon M, Green H (1989) Involucrin in the epidermal cells of subprimates. J Invest Dermatol 92:721–724
Sumitomo S, Kumasa S, Iwai Y, Mori M (1986) Involucrin expression in epithelial tumors of oral and pharyngeal mucosa and skin. Oral Surg Oral Med Oral Pathol 62:155–163
Sun TT, Green H (1976) Differentiation of the epidermal keratinocyte in cell cuture: formation of the cornified envelope. Cell 9:511–521
Thacher SM, Rice RH (1985) Keratinocyte-specific transglutaminase of cultured epidermal cells: relation to cross-linked envelope formation and terminal differentiation. Cell 40:685–695
Walts AE, Said JW (1985) Involucrin, a marker of squamous and urothelial differentiation. An immunohistochemical study on its distribution in normal and neoplastic tissues. J Pathol 145:329–340
Warhol MJ, Antonioli DA, Pinkus GS, Burke L, Rice RH (1982) Immunoperoxidase staining for involucrin: a potential diagnostic aid in cervicovaginal pathology. Hum Pathol 13:1095–1099
Warhol MJ, Roth J, Lucocq JM, Pinkus GS, Rice RH (1985) Immuno-ultrastructural localization of involucrin in squamous epithelium and cultured keratinocytes. J Histochem Cytochem 33:141–149
Watt FM, Phil D (1983) Involucrin and other markers of keratinocyte terminal differentiation. J Invest Dermatol 81:100–103
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Schmid, C., Zatloukal, K., Beham, A. et al. Involucrin expression in breast carcinomas: An immunohistochemical study. Vichows Archiv A Pathol Anat 423, 161–167 (1993). https://doi.org/10.1007/BF01614766
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DOI: https://doi.org/10.1007/BF01614766