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The expression of placental-type glutathione S-tranferase (GST-π) in human cutaneous squamous cell carcinoma and normal human skin

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Abstract

The expression of human placental-type glutathione S-transferase (GST-π) was investigated in human cutaneous squamous cell carcinoma (SCC) and normal skin using Northern blot and immunohistochemical analysis. In Northern blot examination, the expression of GST-π transcript was recognized in all instances, and SCC showed a significantly higher expression of GST-π than normal skin. In immunohistochemical examination, GST-π was stained well in the cytoplasm of all cells of the stratum granulosum, many cells of the stratum spinosum and a few cells of the stratum basale in normal skin. Some cells of the stratum spinosum and almost all cells of the stratum basale showed only a weakly positive or almost negative reaction for GST-π. No nuclear staining of GST-π was obvious in normal epidermal cells. In SCC, many cells showed strong positivity for GST-π in the cytoplasm, and some were obviously accompanied by nuclear staining of GST-π. These findings suggest that GST-π exists mainly in many cells in the upper layers of the normal epidermis and that GST-π is involved in the process of carcinogenesis.

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Authors and Affiliations

  1. Division of Dermatology, Institute for Clinical Research, Nagasaki Chuo National Hospital, 856, Omura, Japan

    K. Shimizu & F. Toriyama

  2. Department of Dermatology, Nagasaki University School of Medicine, 852, Nagasaki, Japan

    H. Yoshida

Authors
  1. K. Shimizu
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  2. F. Toriyama
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  3. H. Yoshida
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Shimizu, K., Toriyama, F. & Yoshida, H. The expression of placental-type glutathione S-tranferase (GST-π) in human cutaneous squamous cell carcinoma and normal human skin. Vichows Archiv A Pathol Anat 425, 589–592 (1995). https://doi.org/10.1007/BF00199348

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  • DOI: https://doi.org/10.1007/BF00199348

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