Summary
Suitability of an ordinary incubation system for the culture of anterior pituitary tissues of mice was examined by electron microscopy. It was found that this system has many advantages over Trowell's standard technique for tissue culture and is particularly suitable for the short-term culture.
In this system the pituitary tissue cultures were maintained well for at least two days in a chemically defined tissue culture medium (TC 199) without any additives. Addition of 20% calf serum to the medium extended the period to up to five days. Any attempts to prolong it further, however, failed because of a massive necrosis and a great loss of the histological integrity.
In the cultured tissues there an enhancement of the LTH cells and a suppression of the other granulated types of cells were observed. The follicular cells were found to hypertrophy and to actively participate in phagocytosis of cell debris.
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Grateful acknowledgement is made for the technical services of Mr. S. Terada, Mr. T. Anzai and Mr. S. Sasaki.
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Yamashita, K. A simple method for the cultivation of anterior pituitary tissues: Electron microscopical evaluation of the cultured tissues. Z. Anat. Entwickl. Gesch. 137, 106–113 (1972). https://doi.org/10.1007/BF00523532
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DOI: https://doi.org/10.1007/BF00523532