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Detection of heat-labile (LT) enterotoxin of enterotoxigenicEscherichia coli by the radial immune hemolysis test: a modification for clinical use

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Abstract

An adaptation for clinical purposes of a radial immune hemolysis test for the detection of the heat-labile (LT) enterotoxin of the enterotoxigenic Escherichia coli is described. The results correlated well with those from Vero cell assay and the passive immune hemolysis test. Because the test is easy, relatively cheap, and as sensitive as other standard procedures, for LT detection, it is particularly suitable for less-equipped laboratories. Also, a large number ofE. coli strains may be examined daily by this technique.

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Authors and Affiliations

  1. Department of Microbiology and Immunology, Institute of Biology, University of Campinas, 13100, Campinas, S.P., Brasil

    T. Yano, M. S. Oliveira, C. F. Fontes, A. C. P. de Almeida & A. F. P. de Castro

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  1. T. Yano
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  2. M. S. Oliveira
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  3. C. F. Fontes
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  4. A. C. P. de Almeida
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  5. A. F. P. de Castro
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Yano, T., Oliveira, M.S., Fontes, C.F. et al. Detection of heat-labile (LT) enterotoxin of enterotoxigenicEscherichia coli by the radial immune hemolysis test: a modification for clinical use. Med Microbiol Immunol 171, 171–178 (1982). https://doi.org/10.1007/BF02123625

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  • DOI: https://doi.org/10.1007/BF02123625

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