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Fluorometrische Bestimmung der unkonjugierten 11-β-Hydroxycorticosteroide in kleinen Serumvolumina (0.1–0.3 ml) und ihre Bedeutung in der pädiatrisch-endokrinologischen Diagnostik

A fluorimetric assay for unconjugated 11-β-hydroxy-corticosteroids in small serum samples (0.1–0.3 ml) and their value in pediatric endocrine diagnosis

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Summary

Following a discussion of the known methods for the determination of 11-β-hydroxycorticosteroids in plasma or serum by fluorimetry a micro-method is described. The advantages of this technique lie in its requirement for minimal amounts of time and laboratory procedures. The criteria for reliability and specificity of this micro-method are identical with those of the macro-methods. Not more than 0.1–0.3 ml of plasma are required for a single determination. The application of this method in the field of pediatic endocrinology is discussed.

Zusammenfassung

Nach einleitender Diskussion der Methoden zur fluorometrischen Bestimmung der 11-β-Hydroxycorticosteroide im Plasma oder Serum wird eine Mikromethode in Anlehnung an de Moor beschrieben. Von den bisherigen Methoden erfordert sie den geringsten Zeit- und Arbeitsaufwand. Die Zuverlässigkeitskriterien von Mikro- und entsprechender Makromethode sind identisch. Das pro Einzelbestimmung erforderliche Serumvolumen beträgt 0,1–0,3 ml. Abschließend wird die Verwendung der Methode in der pädiatrisch-endokrinologischen Diagnostik diskutiert.

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Authors and Affiliations

  1. Universitäts-Kinderklinik Köln, Köln, Deutschland

    K. Geisen, S. Mahal & A. Schwenk

  2. Nuklearmedizinische Abteilung, Klinikum Steglitz der Freien Universität Berlin, Hindenburgdamm 30, 1000, Berlin 45

    K. Geisen

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Geisen, K., Mahal, S. & Schwenk, A. Fluorometrische Bestimmung der unkonjugierten 11-β-Hydroxycorticosteroide in kleinen Serumvolumina (0.1–0.3 ml) und ihre Bedeutung in der pädiatrisch-endokrinologischen Diagnostik. Z. Kinder-Heilk. 107, 175–190 (1969). https://doi.org/10.1007/BF00440234

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  • DOI: https://doi.org/10.1007/BF00440234

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