Summary
The p110gag-myc protein coded for by the retrovirus MC29 was purified 3,000-fold from MC29-Q8 transformed cells by immuno-affinity chromatography using IgG specific for the N-terminal region of the gag protein. Interaction of the protein with DNA fragments was studied by filter binding assay. DNA fragments were obtained from a MC29 DNA clone by restriction endonuclease treatment. Besides the complete DNA provirus the clone contained flanking cellular sequences into which the provirus had integrated. The DNA fragments which were retained by the p110gag-myc protein were eluted from the filter and analyzed by agarose gel electrophoresis. Preferential binding of a DNA fragment originating from the flanking cellular sequences was detected. The protein did not preferentially bind to the viral LTR promoter/enhancer region as suggested by an autoregulatory model, which can therefore no longer be substantiated.
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References
Bishop JM (1985) Trends in oncogenes. Trends in genetics Sept:245–249
Donner P, Greiser-Wilke I, Moelling K (1982) Nuclear localization and DNA-binding of the transforming gene product of avian myelocytomatosis virus. Nature 296:262–266
Hunter T (1984) The epidermal growth factor receptor gene and its product. Nature 311:414–416
Lautenberger JA, Schulz RA, Gavon CF, Tsichlis PN, Papas TS (1981) Molecular, cloning of MC29 transforming sequences. Proc Natl Acad Sci USA 78:1518–1522
Maniatis T, Fritsch EF, Sambrook J (1982) Molecular cloning. Cold Spring Harbor Laboratory Press, Cold Spring Harbor
Moelling K, Heimann B, Beimling P, Rapp U, Sander T (1984) Serine- and threonine-specific protein kinase activities of purified gag-mil and gag-raf proteins. Nature 312:558–561
Moelling K, Pfaff E, Beug H, Beimling P, Bunte T, Schaller HE, Graf T (1985) DNA-binding activity is associated with purified myb proteins from AMV and E26 viruses and is temperature-sensitive for E26 ts mutants. Cell 40:983–990
Rabbitts TH (1985) The c-myc proto-oncogene: involvement in chromosomal abnormality. Trends in Genetics Dec:727–731
Rapp UR, Cleveland JL, Brightman K, Scott A, Ihle JN (1985) Abrogation of IL-3 and IL-2 dependence by recombinant murine retroviruses expressing v-myc oncogenes. Nature 317:434–438
Robertson M (1983) Paradox and paradigm: the message and meaning of myc. Nature 306:733–736
Scheidereit C, Geisse S, Westphal HM, Beato M (1983) The glucocorticoid receptor binds defined nucleotide sequences near the promoter of mouse mammary tumor virus. Nature 304:749–752
Sefton BM (1985) Oncogenes encoding protein kinases. Trends in genetics Nov:306–308
Siebenlist U, Henninghausen L, Batty J, Leder P (1984) Chromatin structure and protein binding in the putative regulatory region of the c-myc gene in Burkitt lymphoma. Cell 34:381–391
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Moelling, K., Sander, T., Lorenz, U. et al. Interaction of the oncogene protein myc with specific DNA fragments. J Cancer Res Clin Oncol 112, 97–99 (1986). https://doi.org/10.1007/BF00404389
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DOI: https://doi.org/10.1007/BF00404389