Skip to main content
SpringerLink
Log in

The electrophoretic mobility test with myelin basic protein, binding of125I-MBP to lymphocytes, and gel electrophoresis pattern of supernatant during incubation

  • Original Contributions
  • Published:
Research in Experimental Medicine

Summary

Lymphocytes from seven patients with malignancies, from seven patients with non-malignancies, and from five healthy persons were incubated with125I-labeled MBP. Binding of MBP to lymphocytes was monitored from 0.5 to 20 h.

The binding ranged from 4 to 7% of the total MBP present and remained fairly constant during the first 4 h of incubation. It dropped considerably at 20 h. Mean percentages of MBP binding were lower in cancer patients than in persons without malignancies.

After incubation, the supernates were examined by gel electrophoresis. The electrophoretic pattern was similar in comparing groups with different diagnoses. A considerable loss of MBP in the terminal supernatant (20 h) was found.

When tested in the electrophoretic mobility test (EM test) with stabilized erythrocytes, supernatant derived from cancer patients produced a somewhat higher mean slowing effect than did supernates from other patients and controls.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Ax W (1979) Tumour diagnosis using electrophoretic mobility test (EMT). In: Flad HD, Herfarth Ch, Betzler M (eds) Immunodiagnosis and immunotherapy of malignant tumors. Springer, Berlin Heidelberg New York, pp 169–184

    Google Scholar 

  2. Böyum A (1976) Isolation of lymphocytes, granulocytes, and macrophages. Scand J Immunol 5:9–15

    Google Scholar 

  3. Caspary EA, Field EJ (1971) Lymphocyte sensitization in cancer: is there a common antigen in human malignant neoplasia? Br Med J 2:612–617

    Google Scholar 

  4. Cohen SR, McKhann GM, Guarnieri M (1975) A radioimmunoassay for myelin basic protein and its use for quantitative measurements. J Neurochem 25:371–376

    PubMed  Google Scholar 

  5. Dickinson JP (1979) A common specific antigen: facts and interpretation. In: Preece AW, Sabolovics D (eds) Cell electrophoresis: clinical application and methodology. Elsevier, North-Holland, pp 279–290

    Google Scholar 

  6. Douwes FR, Spellmann HJ, Mross K, Wolfrum DI (1978) Immundiagnostics of malignant disease. VI. Electrophoretic mobility test (EMT) in malignant melanoma. Oncology 35:163–167

    PubMed  Google Scholar 

  7. Dyson JED, Corbett PJ (1978) Effect of lymphocyte supernatants on the electrophoretic mobility of erythrocytes: significance in cancer diagnosis. Br J Cancer 38:401–410

    PubMed  Google Scholar 

  8. Kaufmann R, Steiner R, Hoffmann W (1979) Lacypher—a new laser cytopheromter for measuring the electrophoretic mobility of particles and cells. In: Preece AW, Sabolovics D (eds) Cell electrophoresis: clinical application and methodology. Elsevier, North-Holland, pp 435–444

    Google Scholar 

  9. Lampert F, Nitzschke U, Zwergel T (1977) Lymphocyte sensitization in childhood solid tumours and lymphoblastic leukaemia, measured by electrophoretic mobility test. Br J Cancer 35:844–849

    PubMed  Google Scholar 

  10. Pritchard JAV (1979) The MEM test—a review. In: Preece AW, Sabolovics D (eds) Cell electrophoresis: clinical application and methodology. Elsevier, North-Holland, pp 335–344

    Google Scholar 

  11. Ritter J, Oehme J (1978) Erfahrungen mit dem Elektrophorese-Mobilitäts-Test bei Kindern. Monatsschr Kinderheilkd 126:556–560

    PubMed  Google Scholar 

  12. Rüchel R, Mesecke S, Wolfrum DI, Neuhoff V (1973) Mikrogelelektrophorese an kontinuierlichen Polyacrylamid-Gradientengelen. I. Herstellung und Eigenschaften von Gradientengelen in Kapillaren, ihre Anwendung zur Proteinfraktionierung und Molekulargewichtsbestimmung. Hoppe Seylers Z Physiol Chem 354:1351–1368

    PubMed  Google Scholar 

  13. Tautz Chr, Bögelspacher HR, Klaissle K (1979) Der Elektrophorese-Mobilitäts-(EM-)Test in der Frühdiagnostik maligner Tumoren. Befunde bei Vorstufen des Zervixkarzinoms und Mammakarzinoms. Onkologie 2:199–203

    PubMed  Google Scholar 

  14. Zwergel T, Nitzschke U, Franke F, Lampert F (1978) Improvements of the electrophoretic mobility test to measure lymphocyte sensitization. Microsc Acta 80:383–390

    PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Division of Hematology/Oncology, Medical Clinic of the University Göttingen, Robert-Koch-Str. 40, D-3400, Göttingen, Germany

    K. Mross & B. Mross

  2. Universitätskinderklinik Göttingen, D-3400, Göttingen, Germany

    A. Kohlschütter

  3. Sonnenberg-Klinik Bad Sooden-Allendorf, D-3437, Bad Sooden-Allendorf, Federal Republic of Germany

    D. I. Wolfrum

Authors
  1. K. Mross
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. B. Mross
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. D. I. Wolfrum
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. A. Kohlschütter
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

This study was supported by BMFT Az. 01 VH088—ZA/NT/MT 225a

Rights and permissions

Reprints and permissions

About this article

Cite this article

Mross, K., Mross, B., Wolfrum, D.I. et al. The electrophoretic mobility test with myelin basic protein, binding of125I-MBP to lymphocytes, and gel electrophoresis pattern of supernatant during incubation. Res. Exp. Med. 177, 245–253 (1980). https://doi.org/10.1007/BF01851215

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01851215

Key words

Search

Navigation