Summary
Propylene glycol is used as a solvent for bile salts in studies on their biologic effects on the liver. While using this solvent (32–64 mmol/l) in the isolated perfused rat liver, we found a significant change in the extramitochondrial redox system as indicated by a fivefold increase of the lactate pyruvate ratio in the perfusate. The increase was due to an increased uptake of pyruvate (0.8 µmol/g/min) and to a release of lactate (1.8 µmol/g/min). The intramitochondrial redox state was affected to a lesser degree as extimated by the β-hydroxybutyrate acetoacetate ratio (twofold increase). These abnormalities resemble those induced by similar concentrations of ethanol. We suggest, therefore, that investigators studying bile acids should be aware of this artifact which causes significant alterations in cellular energy systems and enzyme activities.
Similar content being viewed by others
References
Javitt NB (1966) Cholestasis in rats induced by taurolithocholate. Nature 210:1262–1263
Schölmerich J, Kitamura S, Miyai K (1983) Changes of pattern of biliary bile acids during isolated rat liver perfusion. Biochem Biophys Res Commun 115:518–524
Maugham RJ (1982) A simple rapid method for the determination of glucose, lactate, pyruvate, alanine, β-hydroxybutyrate, and acetoacetate on a single 20µl blood sample. Clin Chim Acta 122:231–240
Bergmeyer HU (1985) Methods of enzymatic analysis, 3rd edn. VCH Verlagsgesellschaft, Weinheim
Comparti M (1978) Ethanol induced liver injury. In: Slater TF (ed) Biochemical mechanisms of liver injury. Academic Press, New York, pp 469–516
Forsander O (1968) Der Einfluß des Äthanols auf den Stoffwechsel der isoliert perfundierten Rattenleber. In: Staib W, Scholz R (eds) Stoffwechsel der isoliert perfundierten Rattenleber. Springer, New York, S 210–215
Soboll S, Heldt HW, Scholz R (1981) Changes in the subcellular distribution of metabolites due to ethanol oxidation in the perfused rat liver. Hoppe-Seyler's Z Physiol Chem 362:247–260
Ruddick JA (1972) Toxicology, metabolism, and biochemistry of 1,2-propanediol. Toxicol Appl Pharmacol 21:102–111
Author information
Authors and Affiliations
Additional information
Supported by NIH grant AM 16110 to K-Miyai
Rights and permissions
About this article
Cite this article
Schölmerich, J., Kitamura, S. & Miyai, K. Effects of propylene glycol on redox state of the perfused rat liver — a note of caution. Res. Exp. Med. 189, 39–42 (1989). https://doi.org/10.1007/BF01856027
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF01856027