Summary
We have undertaken a combined molecular and genetic analysis of the bronze (bz) locus in maize. The bz locus was isolated by a two-step approach involving sequences from the transposable elements Dissociation (Ds) and Activator (Ac). A restriction map of the bz region is presented, identifying the transcribed region and the direction of transcription. We have mapped the sites of two Ds insertions, bz-m2 (DI) and bz-m1, physically and genetically. bz-m2 (DI) is a 3.5 kb insertion in the transcribed region that appears to have arisen by an internal deletion in the 4.5 kb Ac insertion in the mutant bz-m2. bz-m1 is a 1.1 kb insert in the 5′ end of the gene. By correlating the genetic and physical maps, we have been able to orient the restriction map relative to the centromere in chromosome 9 and have obtained a rough estimate of the physical length of a unit of genetic recombination in a microregion of the maize genome.
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References
Aviv H, Leder P (1972) Purification of biologically active globin messenger RNA by chromatography on oligothymidylic acid-cellulose. Proc Natl Acad Sci US 69: 1408–1412
Beckett JB, Burnham CR, Coe EH, Maguire MP, Patterson EP, Phillips RL (1978) Cytogenetic working map. Maize Genet Coop Newsl 52: 129–145
Behrens U, Fedoroff N, Laird A, Muller-Neumann M, Starlinger P, Yoder J (1984) Cloning of the Zea mays controlling element Ac from the wx-m7 allele. Mol Gen Genet 194: 346–347
Benton W, Davis R (1977) Screening lambda gt recombinant clones by hybridization to single plaques in situ. Science 196: 180–182
Coe EH, Hoisington D, Neuffer G (1984) Linkage map of corn Zea mays L). In: O'Brien SJ (ed) Genetic Maps 1984. Cold Spring Harbor Laboratory, New York pp 491–507
Coe EH, Neuffer MG (1977) The genetics of corn. In: Sprague GF (ed) Corn and corn improvement. American Soc of Agronomy, Madison, pp 111–223
Dooner HK (1981) Regulation of the enzyme UFGT by the controlling element Ds in bz-m4, an unstable mutant in maize. Cold Spring Harbor Symp Quant Biol 45: 457–462
Dooner HK, Nelson OE (1977a) Genetic control of UDP glucoseflavonol 3-O-glucosyltransferase in the endosperm of maize. Biochem Genet 15: 509–515
Dooner HK, Nelson OE (1977b) Controlling element-induced alteration in UDPG: flavonoid glucosyltransferase, the enzyme specified by the bronze locus in maize. Proc Natl Acad Sci US 74: 5623–5627
Dooner HK, Nelson OE (1979) Heterogeneous flavonoid glucosyltransferases in purple derivatives from a controlling element-suppressed bronze mutant in maize. Proc Natl Acad Sci US 76: 2369–2371
Döring HP, Tillmann E, Starlinger P (1984) DNA sequence of the maize transposable element Dissociation (Ds). Nature 307: 127–130
Fedoroff N, Wessler S, Shure M (1983) Isolation of the transposable maize controlling elements Ac and Ds. Cell 35: 235–242
Fedoroff N, Furtek D, Nelson O (1984) Cloning of the bronze locus in maize by a simple and generalizable procedure using the transposable controlling element Activator (Ac). Proc Natl Acad Sci US 81: 3825–3829
Frischauf A-M, Lehrach H, Poustka A, Murray N (1983) Lambda replacement vectors carrying polylinker sequences. J Mol Biol 170: 827–842
Galbraith D, Harkins K, Maddox J, Ayres N, Sharma D, Firoozabady E (1983) Rapid flow cytometric analysis of the cell cycle in intact plant tissues. Science 220: 1049–1051
Geiser M, Weck E, Doring H-P, Werr W, Courage-Tebbe U, Tillmann E, Starlinger P (1982) Genomic clones of a wild-type allele and a transposable element-induced mutant allele of the sucrose synthase gene of Zea mays L. EMBO 1: 1455–1460
Gerats A, Bussard J, Coe EH, Larson R (1984) Influence of B and Pl on UDPG: flavonoid-3-O-glucosyltransferase in Zea mays L. Biochem Genet 22: 1161–1169
Hake S, Walbot V (1980) The genome of Zea mays, its organization and homology to related grasses. Chromosoma 79: 251–270
Karn J, Brenner S, Barnett L, Cesarini G (1980) Novel bacteriophage lambda cloning vector. Proc Natl Acad Sci US 77: 5172–5176
Larson R, Coe EH (1977) Gene-dependent flavonoid glucosyltransferase in maize. Biochem Genet 15: 153–156
Maniatis T, Fritsch E, Sambrook (1982) Molecular Cloning: A laboratory manual. Cold Spring Harbor Laboratory, New York
McClintock B (1951) Mutable loci in maize. Carnegie Inst Wash Yrbk 50: 174–181
McClintock B (1952) Chromosome organization and gene expression. Cold Spring Harbor Symp Quant Biol 16: 13–47
McClintock B (1955) Controlled mutation in maize. Carnegie Inst Wash Yrbk 54: 245–255
McClintock B (1956) Mutation in maize. Carnegie Inst Wash Yrbk 55: 323–332
McClintock B (1962) Topographical relations between elements of control systems in maize. Carnegie Inst Wash Yrbk 61: 448–461
McClintock B (1963) Further studies of gene-control systems in maize. Carnegie Inst Wash Yrbk 62: 486–493
Mottinger J (1973) Unstable mutants of bronze induced by premeiotic X-ray treatment in maize. Theor Appl Genet 43: 190–195
Mueller-Neumann M, Yoder JI, Starlinger P (1984) The DNA sequence of the transposable element Ac of Zea mays L. Mol Gen Genet 198: 19–24
Murray M, Thompson W (1980) Rapid isolation of large molecular weight plant DNA. Nucleic Acid Res 8: 4321–4326
Pohlman R, Fedoroff N, Messing J (1984a) The nucleotide sequence of the maize controlling element Activator. Cell 37: 635–643
Pohlman R, Fedoroff N, Messing J (1984b) Correction: nucleotide sequence of Ac. Cell 39: 417
Southern E (1975) Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol 98: 503–517
Vieira J, Messing J (1982) The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic primers. Gene 19: 259–268
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Dooner, H.K., Weck, E., Adams, S. et al. A molecular genetic analysis of insertions in the bronze locus in maize. Molec. Gen. Genet. 200, 240–246 (1985). https://doi.org/10.1007/BF00425430
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DOI: https://doi.org/10.1007/BF00425430