Summary
IS2 has been marked genetically by the in vitro insertion into its HindIII site of a 3.3 Kb HindIII fragment of Tn5 conferring resistance to kanamycin. The transposition of the IS2::Km, thus obtained, to λ has been found and insertion sites were characterised. Each of ten independent IS2::Km insertions were found at the same site at 61.2% of the λ map, always in the same orientation (orientation II relative to the xis gene). The integration sites of IS2::Km in five of the kanamycin-transducing phages were determined by DNA sequence analysis, and were found to be identical at the nucleotide level. Further transposition of IS2::Km from λ to the bacterial chromosome was demonstrated.
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Communicated by E. Bautz
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Saint-Girons, I., Fritz, H.J., Shaw, C. et al. Integration specificity of an artificial kanamycin transposon constructed by the in vitro insertion of an internal Tn5 fragment into IS2 . Molec. Gen. Genet. 183, 45–50 (1981). https://doi.org/10.1007/BF00270136
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DOI: https://doi.org/10.1007/BF00270136