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Localization of a gene for human α-galactosidase B (=N-Acetyl-α-D-Galactosaminidase) on chromosome 22

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Summary

The localization of the structural gene for human α-galactosidase B (=N-acetyl-α-galactosaminidase) was investigated by means of man-Chinese hamster and man-mouse somatic cell hybrids. The hybrid clones were analyzed for chromosomes and for a large number of known enzyme markers. The lysates of the hybrid cells were treated with Sepharose-coupled antihuman α-galactosidase B and the activity of the adsorbed enzyme was measured on the Sepharose beads as N-acetyl-α-galactosominidase. The results show that the structural gene for human α-galactosidase B is situated on chromosome 22, and that there is no structural relationship between human α-galactosidase A and human α-galactosidase B.

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Authors and Affiliations

  1. Laboratory of Biochemistry, B.C.P. Jansen Institute, University of Amsterdam, Plantage Muidergracht 12, 1018 TV, Amsterdam, The Netherlands

    P. G. de Groot & J. M. Tager

  2. Division of Cell Biology and Cell Genetics, Erasmus University, Rotterdam, The Netherlands

    A. Westerveld

  3. Department of Human Genetics, University of Leiden, Wassenaarseweg 72, Leiden, The Netherlands

    P. Meera Khan

Authors
  1. P. G. de Groot
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  2. A. Westerveld
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  3. P. Meera Khan
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  4. J. M. Tager
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de Groot, P.G., Westerveld, A., Khan, P.M. et al. Localization of a gene for human α-galactosidase B (=N-Acetyl-α-D-Galactosaminidase) on chromosome 22. Hum Genet 44, 305–312 (1978). https://doi.org/10.1007/BF00394295

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  • DOI: https://doi.org/10.1007/BF00394295

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