Skip to main content
SpringerLink
Log in

A simple combined Ag-I/RHG technique for human metaphase chromosomes

  • Short Communications
  • Published:
Human Genetics Aims and scope Submit manuscript

Summary

A combination of the Ag-I staining method of the human nucleolus organizer regions (NORs) with an RHG banding method is described. This technique gives a reliable identification of each of the Ag+NOR acrocentric chromosomes. Its probable chemical nature is discussed.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

References

  • Dutrillaux B, Lejeune J (1975) New technique in the study of the human chromosomes: Methods and application. Ann Hum Genet 5: 119–156

    Google Scholar 

  • Bloom SE, Goodpasture C (1976) An improved technique for selective staining of nucleolus organizer regions in human chromosomes. Hum Genet 34:199–206

    Google Scholar 

  • Hens L, Kirsch-Volders M, Arrighi FE, Susanne C (1980) Relationship between measured chromosome distribution parameters and Ag-staining of nucleolus organizer regions. Hum Genet 53:363–370

    Google Scholar 

  • Lau YF, Pfeiffer RA, Arrighi E, Hsu TC (1978) Combination of silver and fluorescent staining for metaphase chromosomes. Am J Hum Genet 30:76–79

    Google Scholar 

  • Summer AT (1982) The nature and mechanism of chromosome banding. Cancer Genet Cytogenet 6:59–87

    Google Scholar 

  • Tantravahi R, Miller DA, Miller OJ (1977) Ag-staining of nucleolus organizer regions of chromosome after Q-, C-, G-, or R banding procedures. Cytogenet Cell Genet 18:364–369

    Google Scholar 

  • Zakharov AF, Davudov AZ, Benjush VA, Egolina NA (1982) Genetic determination of NOR activity in human lymphocytes from twins. Hum Genet 60:24–29

    Google Scholar 

  • Zankl H, Bernhardt S (1977) Combined silver staining of the nucleolus organizing regions and Giemsa banding in human chromosomes. Hum Genet 37:79–80

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Instituto Biologia de la Altura Universidad Nacional de Jujuy, Avda. Bolivia 2335, 4600, S. S. de Jujuy, Argentina

    J. E. Dipierri

  2. Laboratoire de Cytogénétique, Centre de Transfusion Sanguine, 25 Bd. Pasteur, F-42023, Saint-Etienne, France

    J. Fraisse

Authors
  1. J. E. Dipierri
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. J. Fraisse
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Dipierri, J.E., Fraisse, J. A simple combined Ag-I/RHG technique for human metaphase chromosomes. Hum Genet 64, 286–287 (1983). https://doi.org/10.1007/BF00279413

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00279413

Keywords

Search

Navigation