Summary
Ultrastructure of mitotic cells in human lymph node germinal centers was deliberately studied in contrast to that of plasma cells in mitosis which were rarely found in medullary cords or lymphatic sinuses of the same materials. It was demonstrated that the mitotic cells in germinal centers are evidently different from the latter in the absence of lamellary arranged endoplasmic reticulum with ribosomes and Golgi apparatus, and are quite similar to the ultrastructure of thymic lymphocytes in mitosis reported by Murray et al. It should be concluded from these findings that the cells produced locally within the germinal centers in human lymph nodes are lymphocytic as has been repeatedly suggested by the authors.
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References
Burtin, P., Buffe, D.: Synthesis of human immunoglobulins in germinal centers of lymphoid organs. J. Immunol. 98, 536–542 (1967).
Cardno, S. C., Steiner, J. W.: Improvement of staining technics for thin sections epoxyembedded tissue. Amer. J. clin. Path. 43, 1–8 (1965).
Caulfield, J. B.: Effect of varying the vehicles for OsO4 in tissue fixation. J. biophys. biochem. Cytol. 3, 827–830 (1957).
Congdon, C. C., Makinodan, T.: Splenic white pulp alteration after antigen injection: relation to time of serum antibody production. Amer. J. Path. 39, 697–709 (1961).
Conway, E. A.: Cyclic changes in lymphatic nodules. Anat. Rec. 69, 487–513 (1937).
Douglas, S. D., Hoffman, P. F., Borjeson, J., Chessin, L. N.: Studies on human peripheral blood lymphocytes in vitro: III. Fine structural features of lymphocyte transformation by pokeweed mitogen. J. Immunol. 98, 17–30 (1967).
Flemming, W.: Studien über Regeneration der Gewebe. Arch. mikr. Anat. 24, 50–97 (1885).
Ishii, Y., Mori, M., Fujimoto, T.: Studies on the germinal center II. Immunofluorescence study of germinal centers in mouse lymph nodes during the secondary immune response. Tohoku J. exp. Med. In contribution.
Kimura, A., Onoé, T.: Ultrastructure of hepatocytes during mitosis. Gann (In contribution).
La Via, M. F., Vatter, A. E., Claman, H. N., Brunstetter, F. H.: Effects of antilymphocyte serum and phytohemagglutinin upon cultures of human thymus and peripheral blood lymphoid cells. Lab. Invest. 18, 763–770 (1968).
Lennert, K., Caser, R., Müller, H. K.: Electron microscopic studies of germinal centers in man. In: Germinal centers in immune responses (H. Cottier, N. Odartchenko, R. Schindler, and C. C. Congdon, eds.), p. 49–59. Berlin-Heidelberg-New York: Springer 1967.
Luft, J. H.: Improvements in epoxy resin embedding methods. J. biophys. biochem. Cytol. 9, 409–414 (1961).
Millikin, P. D.: Anatomy of germinal centers in human lymphoid tissues. Arch. Path. 82, 499–505 (1966).
Mitrou, P. S., Queisser, W., Lennert, K., Sandritter, W.: Kombinierte autoradiographischcytophotometrische Untersuchungen von Keimzentrumzellen der menschlichen Tonsille. Virchows Arch. Abt. B. Zellpath. 3, 156–170 (1969).
Mori, M., Ishii, Y., Onoé, T.: Studies on the germinal center I. Ultrastructural study of germinal centers in human lymph nodes with correspondence to the zonal differentiation. J. Reticuloendoth. Soc. 6, 140–157 (1969).
Murray, R. G., Murray, A. S., Pizzo, A.: The fine structure of mitosis in rat thymic lymphocytes. J. Cell Biol. 26, 601–620 (1965).
Nossal, G. J. V., Mäkälä, O.: Autoradiographic studies on the immune response. I. The kinetics of plasma cell proliferation. J. exp. Med. 115, 209–230 (1962).
Ortega, L. G., Mellors, R. C.: Cellular site of formation of gamma globulin. J. exp. Med. 106, 627–640 (1957).
Reynolds, E. S.: The use of lead citrate at high pH as an electron opaque stain in electron microscopy. J. Cell Biol. 17, 208–212 (1963).
Sordat, B., Moser, H., Gerber, H., Cottier, H.: Differentiation pathway within germinal centers of human tonsils. In: Lymphoid tissue and germinal centers in immune response (L. Fiore-Donati, and M. G. Hanna, Jr., eds.), p. 73–82. New York: Plenum 1969.
—, Sordat, M., Hess, M. W., Stoner, R. D., Cottier, H.: Specific antibody within lymphoid germinal center cells of mice after primary immunization with horseradish peroxidase: A light and electron microscopic study. J. exp. Med. 131, 77–89 (1970).
Watson, M. L.: Staining of tissue sections for electron microscopy with heavy metals. J. biophys. biochem. Cytol. 4, 475–478 (1958).
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Supported by Grant in Aid from the Ministry of Education of Japan (69-9254).
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Mori, M., Ishii, Y. & Onoé, T. Studies on the germinal center. Z. Zellforsch. 112, 158–165 (1971). https://doi.org/10.1007/BF00331838
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DOI: https://doi.org/10.1007/BF00331838