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Effect of glucagon on the secretory process in the rat exocrine pancreas

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Summary

Glucagon was infused into conscious rats in doses of 10 to 80 μg/h for periods up to 24 h. The effect on the secretory process of the exocrine pancreas was studied in vitro using isolated pancreatic lobules. A pronounced inhibition of the rate of protein synthesis and discharge of stored and newly synthesized proteins combined with increased enzyme content in the pancreas were observed after 30 min infusion. This effect was absent after longer infusion periods of up to six hours. After 12 to 24 h infusions a marked degranulation and decrease in enzyme content was observed. While the rate of protein synthesis was not significantly enhanced, both the basal and stimulated discharge of enzymes from the pancreas were increased. The results suggest a biphasic response of the pancreas to prolonged glucagon infusion.

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References

  • Adler, G., Kern, H.F.: Regulation of exocrine pancreatic secretory process by insulin in vivo. Horm. Metab. Res. 7, 290–296 (1975)

    Google Scholar 

  • Bernfeld, P.: Amylases, α and β. Meth. Enzymol. 1, 149–158 (1955)

    Google Scholar 

  • Bieger, W., Martin-Achard, A., Bassler, M., Kern, H.F.: Studies on intracellular transport of secretory proteins in the rat exocrine pancreas. IV. Stimulation by in vivo infusion of caerulein. Cell Tiss. Res. 165, 435–453 (1976)

    Google Scholar 

  • Condon, J.R., Knight, M., Day, J.L.: Glucagon therapy in acute pancreatitis. Brit. J. Surg. 60, 509–511 (1973)

    Google Scholar 

  • Condon, R.E., Woods, J.H., Poulin, T.L., Wagner, W.G., Pissiotis, C.A.: Experimental pancreatitis treated with glucagon or lactated Ringer solution. Arch. Surg. 109, 154–158 (1974)

    Google Scholar 

  • Dreiling, D.A., Janowitz, H.D.: The effect of glucagon on gastric secretion in man. Gastroenterol. 36, 580–581 (1959)

    Google Scholar 

  • Dürr, H.K., Bode, J.Ch.: Klinik und Therapie der akuten Pankreatitis. Leber, Magen, Darm 6, 282–293 (1976)

    Google Scholar 

  • Dyck, W.P., Janowitz, H.D.: Effect of glucagon on hepatic bile secretion in man. Gastroneterol. 60, 400–404 (1971)

    Google Scholar 

  • Dyck, W.P., Rudick, J., Hoexter, B., Janowitz, H.D.: Influence of glucagon on pancreatic exocrine secretion. Gastroenterol. 56, 531–537 (1969)

    Google Scholar 

  • Dyck, W.P., Texter, E.C., Lasater, J.M., Hightower, N.C.: Influence of glucagon on pancreatic exocrine secretion in man. Gastroenterol. 58, 532–538 (1970)

    Google Scholar 

  • Ferner, H.: Das Inselsystem des Pankreas, pp. 96–101. Stuttgart: Georg Thieme 1952

    Google Scholar 

  • Ferner, H.: Die Dissemination der Hodenzwischenzellen und der Langerhansschen Inseln als funktionelles Prinzip für die Samenkanälchen und das exokrine Pankreas. Z. mikr.-anat. Forsch. 63, 35–52 (1957)

    Google Scholar 

  • Fujita, T., Yanatori, Y., Murakami, T.: Insulo-acinar axis, its vascular basis and its functional and morphological changes caused by CCK-PZ and caerulein. In: Endocrine gut and pancreas (T. Fujita, ed.), pp. 347–357. Amsterdam: Elsevier 1976

    Google Scholar 

  • Glässer, A.G., Dorigotti, L., Vaghi, G.: Inhibitory activity of glucagon on caerulein exocrine stimu-lation, independent of hyperglycemia. Adv. exp. Med. and Biol. 21, 347–355 (1972)

    Google Scholar 

  • Henderson, J.R.: Why are the islets of Langerhans? Lancet 1969 I, 469–470

  • Hummel, B.C.: A modified spectrophotometric determination of chymotrypsin, trypsin and thrombin. Canad. J. Biochem. 37, 1393–1399 (1959)

    Google Scholar 

  • Iwatsuki, K., Ono, H., Hashimoto, K.: Effects of glucagon on pancreatic secretion in the dog. Clin. exp. Pharm. Physiol. 3, 59–65 (1976)

    Google Scholar 

  • Jamieson, J.D., Palade, G.E.: Synthesis, intracellular transport and discharge of secretory proteins in stimulated pancreatic exocrine cells. J. Cell Biol. 50, 135–158 (1971)

    Google Scholar 

  • Jarett, L.: In vivo and in vitro effect of glucagon on DL-leucine-1-C14 incorporation into protein of rat pancreas. Proc. Soc. exp. Biol. (N.Y.) 114, 550–554 (1963)

    Google Scholar 

  • Jarett, L., Lacy, P.E.: Effect of glucagon on the acinar portion of the pancreas. Endocrinol. 70, 867–873 (1962)

    Google Scholar 

  • Kasper, H., Fleischer, K.: Der Einfluß von Glukagon auf die Lipaseaktivität im Serum bei Pankreatitis. Dtsch. med. Wschr. 97, 1124–1126 (1972)

    Google Scholar 

  • Knight, M.J., Condon, J.R., Smith, R.: Possible use of glucagon in the treatment of pancreatitis. Brit. med. J. 1971 II, 440–442

    Google Scholar 

  • Kronborg, O., Jørgensen, P.M., Bülow, S.: A controlled randomized trial of glucagon in treatment of first attack of severe acute pancreatitis without associated biliary disease, interim report. Scand. J. Gastroent. 38, 1031 (1976)

    Google Scholar 

  • Lankisch, P.G., Winckler, K., Bokermann, M., Schmidt, H., Creutzfeldt, W.: The influence of glucagon on acute experimental pancreatitis in the rat. Scand. J. Gastroent. 9, 725–729 (1974)

    Google Scholar 

  • Lankisch, P.G., Winckler, K., Schmidt, H.: Glucagon-Behandlung der akuten Pankreatitis. Dtsch. med. Wschr. 100, 845–846 (1975)

    Google Scholar 

  • Nakajima, S., Magee, D.F.: Inhibition of exocrine pancreatic secretion by glucagon and D-glucose given intravenously. Canad. J. Physiol. Pharmacol. 48, 299–305 (1969)

    Google Scholar 

  • Necheles, H., Walker, L., Mabin, W.: Effects of glucagon on external secretion of the pancreas. Amer. J. Physiol. 191, 595–597 (1957)

    Google Scholar 

  • Papp, M., Ribet, A., Fodor, I., Németh, P.E., Fehér, S., Horvath, J.E., Folly, G.: Glucagon treatment of experimental acute pancreatitis. Acta med. Sci. hung. 32, 105–116 (1975)

    Google Scholar 

  • Richards, G.M.: Modifications of the diphenylamine reaction giving increased sensitivity and simplicity in the estimation of DNA. Analyt. Biochem. 57, 369–376 (1974)

    Google Scholar 

  • Robinson, R.M., HarIis, K., Hlad, C.J., Jr., Eisemann, B.: Effect of glucagon on gastric secretion. Proc. Soc. exp. Biol. (N.Y.) 96, 518–525 (1957)

    Google Scholar 

  • Samols, E., Marri, G., Marks, V.: Interrelationship of glucagon, insulin and glucose. Diabetes 15, 855–866 (1966)

    Google Scholar 

  • Scheele, G.A., Palade, G.E.: Studies on the guinea pig pancreas. Parallel discharge of exocrine enzyme activities. J. biol. Chem. 250, 2660–2670 (1975)

    Google Scholar 

  • Shaw, H.M., Heath, T.J.: The effect of glucagon on the formation of pancreatic juice and bile in the rat. Canad. J. Physiol. Pharmacol. 51, 1–5 (1973)

    Google Scholar 

  • Söling, H.D., Unger, K.O.: The role of insulin in the regulation of α-amylase synthesis in the rat pancreas. Europ. J. clin. Invest. 2, 199–212 (1972)

    Google Scholar 

  • Steffen, Ch., Goebell, H., Baltzer, G., Dürr, H.K.: Untersuchungen zum Hemmeffekt von Glukagon auf die Pankreassekretion des Menschen. Verh. dtsch. Ges. inn. Med. 78, 1423–1425 (1972)

    Google Scholar 

  • Sweeting, J.G.: The effect of glucagon on bile and pancreatic juice. Gastroenterol. 54, 1276 (1965)

    Google Scholar 

  • Verduin, P.A., Punt, J.M.M.M., Kreutzer, H.H.: Studies on the determination of lipase activity. Clin. chim. Acta 46, 11–19 (1973)

    Google Scholar 

  • Waterworth, M.W., Barbezat, G.O., Bank, S.: Glucagon in treatment of acute pancreatitis. Lancet 1974 I, 1231

  • Youngs, G.: Hormonal control of pancreatic endocrine and exocrine secretion. Gut 13, 154–161 (1972)

    Google Scholar 

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Authors and Affiliations

  1. Department of Anatomy, University of Heidelberg, Heidelberg, Germany

    Guido Adler

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  1. Guido Adler
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Dedicated to Professor Helmut Ferner, Vienna, Austria, on the occasion of his 65th birthday

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Adler, G. Effect of glucagon on the secretory process in the rat exocrine pancreas. Cell Tissue Res. 182, 193–204 (1977). https://doi.org/10.1007/BF00220588

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