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A monoamine in the gustatory cell of the frog's taste organ

A fluorescence histochemical and electron microscopic study

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Summary

Fluorescence histochemistry reveals that in the frog's taste organ a yellow fluorescence is regularly observed at the most basal region of the sensory epithelium. The fluorescence has a strong intensity, but it fades rapidly upon the UV-irradiation. The peak of the emission spectrum is at 520 mμ. Following reserpine treatment the yellow fluorescence is markedly reduced, but not depleted completely. From these characteristics the monoamine fluorescence is regarded as representing 5-HT (serotonin).

The ultrastructural study on sensory epithelia shows that the terminal portions of gustatory cell processes are localized at the basal region. These portions are filled with dense cored vesicles (700–1000 Å in diameter) and frequently opposed with nerve fibers penetrating into the epithelium. The gustatory cell processes are also interposed between the terminal portions or nerve fibers. The cytoplasm of the gustatory cell process is characterized by many mitochondria, fine filaments and glycogen particles, but contains few cored vesicles. The distribution of terminal portions of gustatory cell processes seems to correspond fairly well to that of the monoamine fluorescence observed discontinuously along the basal lamina. Accordingly it is concluded that the fluorigenic monoamine is localized in the cored vesicles of the gustatory cell.

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References

  • Björklund, A., Falck, B., Stenevi, U.: Classification of monoamine neurones in the rat mesencephalon; distribution of a new monoamine neurone system. Brain Res. 32, 269–285 (1971)

    Google Scholar 

  • Caspersson, T., Hillarp, N.-Å., Ritzén, M.: Fluorescence microspectrophotometry of cellular catecholamines and 5-hydroxytryptamine. Exp. Cell Res. 42, 415–428 (1966)

    Google Scholar 

  • DeHan, R. S., Graziadei, P. P. C.: Functional anatomy of frog's taste organs. Experientia (Basel) 27, 823–826 (1971)

    Google Scholar 

  • DeHan, R. S., Graziadei, P. P. C.: The innervation of frog's taste organ. A histochemical study. Life Sci. 13, 1435–1449 (1973)

    Google Scholar 

  • Falck, B., Hillarp, N.-A., Thieme, G., Torp, A.: Fluorescence of catecholamines and related compounds condensed with formaldehyde. J. Histochem. Cytochem. 10, 348–354 (1962)

    Google Scholar 

  • Graziadei, P. P. C., DeHan, R. S.: The ultrastructure of frogs' taste organs. Acta anat. (Basel) 80, 563–603 (1971)

    Google Scholar 

  • Huber, J. D., Parker, F., Odland, G. F.: A basic fuchsin and alkalinized methylene blue rapid stain for epoxy-embedded tissue. Stain Technol. 43, 83–87 (1968)

    Google Scholar 

  • Lorenzo, A. J. de: Electron microscopic observations on the taste buds of the rabbit. J. biophys. biochem. Cytol. 4, 143–163 (1958)

    Google Scholar 

  • Murray, B. G., Murray, A.: Fine structure of taste buds of rabbit foliate papillae. J. Ultrastruct. Res. 19, 327–353 (1967)

    Google Scholar 

  • Murray, R. G., Murray, A., Fujimoto, S.: Fine structure of gustatory cells in rabbit taste buds. J. Ultrastruct. Res. 27, 444–461 (1969)

    Google Scholar 

  • Nada, O., Hirata, K.: The occurrence of the cell type containing a specific monoamine in the taste bud of the rabbit's foliate papilla. Histochemistry (1975) in press

  • Potter, L. T.: Role of intraneuronal vesicles in the synthesis, storage, and release of noradrenaline. Suppl. 3 to Circulat. Res. 20 and 21, 13–24 (1967)

    Google Scholar 

  • Reutter, K.: Die Geschmacksknospen des Zwergwelses Amiurus nebulosus (Lesueur). Morphologische und histochemische Untersuchungen. Z. Zellforsch. 120, 280–308 (1971)

    Google Scholar 

  • Uga, S.: The fine structure of gustatory receptors and their synapses in frog's tongue. Symp. Cell Chem. 16, 75–85 (1966)

    Google Scholar 

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Authors and Affiliations

  1. Department of Anatomy, Faculty of Medicine, Kyushu University, Fukuoka, Japan

    Kazuho Hirata

  2. Department of Medical Technology, Kyushu University School of Health Sciences, Fukuoka, Japan

    Osami Nada

Authors
  1. Kazuho Hirata
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  2. Osami Nada
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Additional information

These results were reported in a preliminary form to the October, 1974 meeting of the Japan Society of Histochemistry and Cytochemistry.

The authors gratefully acknowledge the support and helpful advice of Prof. Dr. T. Kanaseki.

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Hirata, K., Nada, O. A monoamine in the gustatory cell of the frog's taste organ. Cell Tissue Res. 159, 101–108 (1975). https://doi.org/10.1007/BF00231999

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  • DOI: https://doi.org/10.1007/BF00231999

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