Summary
In the retina of the rainbow trout (Salmo gairdneri, Richardson) two types of microtubular structures are demonstrated. Besides the normal type of microtubules (about 200 Å in diameter), occurring in all cell types of the retina, a second type is described which is termed microtubule-like structure (MLS) because of its extracellular localization. These MLS have a diameter of about 250 Å under the same preparative conditions in which the normal microtubules appear 180–200 Å thick. The interspace between the tubules is smaller than between the microtubules. Specific MLS to membrane associations exist, which are analyzed by serial sectioning and tilting procedures. It is suggested that the MLS have their origin at small membranous extrusions of the plasmalemma. These extrusions could contain nucleation sites for the MLS-formation within the extracellular space. It remains unknown which cell type produces the MLS proteins and which factors are responsible for the aggregation of the subunits to intact MLS.
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References
Bardele, C.F.: Struktur, Biochemie und Funktion der Mikrotubuli. Cytobiologie 7, 442–488 (1973)
Behnke, O.: An outer component of microtubules. Nature (Lond.) 257, 709–710 (1975)
Bird, M.M.: Microtubule-synaptic vesicle associations in cultured rat spinal cord neurons. Cell Tiss. Res. 168, 101–115 (1976)
Feit, H., Dutton, G.R., Barondes, S.H., Shelanski, M.L.: Microtubule protein: identification in and transport to nerve endings. J. Cell Biol. 51, 138–147 (1971)
Feit, H., Shelanski, M.L.: Is tubulin a glycoprotein? Biochem. biophys. Res. Commun. 66, 920–927 (1975)
Gray, E.G.: Presynaptic microtubules and their association with synaptic vesicles. Proc. roy. Soc. B 190, 369–372 (1975)
Gray, E.G.: Microtubules in synapses of the retina. J. Neurocytol. 5, 361–370 (1976)
Jacobs, M., Bennett, P.M., Dickens, M.J.: Duplex microtubule is a new form of tubulin assembly induced by polycations. Nature (Lond.) 257, 707–709 (1975)
Martinez-Palomo, A.: The surface coat of animal cells. Int. Rev. Cytol. 29, 29–75 (1970)
Malus, A.J., Walters, B.B., Mughal, S.: Immunohistochemical demonstration of tubulin associated with microtubules and synaptic junctions in mammalian brain. J. Neurocytol. 4, 733–744 (1975)
Roberts, K.: Cytoplasmic microtubules and their functions. Progr. Biophys. molec. Biol. 28, 371–420 (1974)
Shelanski, M.: Methods for the neurochemical study of microtubules. Res. Methods in Neurochemistry (N. Marks and R. Rodnight, eds.), Vol. 2, pp. 281–300. New York-London: Plenum Press 1974
Stell, W.K.: Horizontal cell axons and axon terminals in goldfish retina. J. comp. Neurol. 159, 503–520 (1975)
Westrum, L.E., Gray, E.G.: Microtubules and membrane specializations. Brain Res. 105, 547–550 (1976)
Wolburg, H.: Influence of ouabain on the fine structure of teleost retina. Acta neuropath. (Berl.) 34, 255–266 (1976)
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This work was supported by grant Wo 215/3 from the Deutsche Forschungsgemeinschaft
The authors wish to thank Prof. Dr. W. Schlote for critically reading the manuscript, Mrs. B. Sabrowski for typing it, and Dr. B. Boschek for correcting the English text
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Wolburg, H., Kurz-Isler, G. Microtubules and extracellular microtubule-like structures in the retina of the rainbow trout. Cell Tissue Res. 177, 127–140 (1977). https://doi.org/10.1007/BF00221124
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DOI: https://doi.org/10.1007/BF00221124