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An autoradiographic study on the biosynthesis of the capillary basal lamina in the chick embryo telencephalon

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Summary

The elaboration of the basal lamina of telencephalic capillaries has been studied by autoradiography in chick embryos from day 16 to 19 of incubation. The cerebral vascular basal lamina is actively synthesized during the period of these stages of development. The animals were sacrificed 2, 15, 40 and 180 min after intravenous injection of 3H-proline. The hydroxylation of proline to hydroxyproline in the “secretory” cells permits their identification.

Our observations suggest that endothelial cells, pericytes and perhaps glial cells participate in this elaboration. The various stages of the synthesis are performed sequentially in the ergastoplasm and then in the Golgi apparatus before the secretory material is released by exocytosis.

Résumé

L'élaboration de la lame basale des capillaires télencéphaliques a été étudiée en autoradiographie chez des embryons de Poulet âgés de 16 à 19 jours d'incubation. C'est au cours de cette période du développement que la basale des vaisseaux cérébraux est activement synthétisée.

Les animaux furent sacrifiés 2,15, 40 et 180 min après le début de l'injection intraveineuse d'une solution de proline tritiée. L'identification des cellules participant à la synthèse de la basale est facilitée par le fait que l'hydroxylation de la proline en hydroxyproline y est réalisée.

Nos observations nous permettent de suggérer une participation active des cellules endothéliales, des péricytes, et peut-être des cellules gliales, à l'élaboration de la lame basale. Les différentes étapes de cette synthèse ont lieu, par ordre chronologique, dans l'ergastoplasme, puis dans l'appareil de Golgi, avant que le produit élaboré ne soit libéré dans l'espace périvasculaire par exocytose.

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References

  • Andres, G.A., Morgan, C., Hsu, K.C., Rifkind, R.A., Seegal, B.C.: Electron microscopic studies of experimental nephritis with ferritin-conjugated antibody. The basement membranes and cisternae of visceral epithelial cells in nephritic rat glomeruli. J. exp. Med. 115, 929–936 (1972)

    Google Scholar 

  • Bär, T., Wolff, J.R.: The formation of capillary basement membranes during internal vascularization of the rat's cerebral cortex. Z. Zellforsch. 133, 231–248 (1972)

    Google Scholar 

  • Bendayan, M., Sandborn, E., Rasio, E.: Studies of the capillary basal lamina. II. Preparation, chemical composition and metabolic properties. Lab. Invest. 32, 768–772 (1975)

    Google Scholar 

  • Booz, K.H., Desaga, U., Feising, T.: Über die Entstehung der Basalmembranlabyrinthe. Eine licht- und elektronenmikroskopische Untersuchung. Z. Anat. Entwickl.-Gesch. 143, 185–203 (1974)

    Google Scholar 

  • Breipohl, W.: Entwicklung und gliöse Beziehungen der Basalmembran der Kapillaren des Mäusegehirns. Acta anat. (Basel) 69, 393–408 (1968)

    Google Scholar 

  • Caley, D.W., Maxwell, D.S.: Development of the blood vessels and extracellular spaces during postnatal maturation of rat cerebral cortex. J. comp. Neurol. 138, 31–48 (1970)

    Google Scholar 

  • Cutroneo, K.R., Guzman, N.A., Sharawy, M.M.: Evidence for a subcellular vesicular site of collagen prolyl hydroxylation. J. biol. Chem. 249, 5989–5994 (1974)

    Google Scholar 

  • Delorme, P.: Etude ultrastructurale du développement des capillaires, de leur environnement et des modifications de leur perméabilité à la peroxydase du Raifort dans le télencéphale du Poulet au cours de l'embryogenèse et de la croissance postnatale. Thèse Doctorat ès Sciences, Université de Nancy I, Nancy, France (1971)

    Google Scholar 

  • Delorme, P., Gayet, J., Grignon, G.: Ultrastructure study on capillary exchanges in the developing telencephalon of the chicken. Brain Res. 22, 269–283 (1970)

    Google Scholar 

  • Delorme, P., Grignon, G., Gayet, J.: Ultrastructure des capillaires dans le télencéphale du Poulet au cours de l'embryogenèse et de la croissance postnatale. Z. Zellforsch. 87, 592–602 (1968)

    Google Scholar 

  • Donahue, S.: Relationship between fine structure and function of blood vessels in the central nervous system of rabbit fetuses. Amer. J. Anat. 115, 17–26 (1964)

    Google Scholar 

  • Donahue, S., Pappas, G.D.: The fine structure of capillaries in the cerebral cortex of the rat at various stages of development. Amer. J. Anat. 108, 331–338 (1961)

    Google Scholar 

  • Hamburger, V., Hamilton, H.L.: A series of normal stages in the development of the chick embryo. J. Morph. 88, 49–92 (1951)

    Google Scholar 

  • Kefalides, N.A.: Basement membranes: structural and biosynthetic considerations. J. invest. Derm. 65, 85–92 (1975)

    Google Scholar 

  • Kefalides, N.A., Denduchis, B.: Structural components of epithelial and endothelial basement membranes. Biochem. 8, 4613–4621 (1969)

    Google Scholar 

  • Kiss, F.A.: Experimental stimulation of vascularization of brain tissue in chick embryos. Acta biol. Acad. Sci. hung. 15, 181–190 (1964)

    Google Scholar 

  • Kurtz, S.M.: The electron microscopy of the developing human renal glomerulus. Exp. Cell Res. 14, 355–367 (1958)

    Google Scholar 

  • Kurtz, S.M.: The fine structure of the lamina densa. Lab. Invest. 10, 1189–1207 (1961)

    Google Scholar 

  • Kurtz, S.M., Feldman, J.D.: Experimental studies on the formation of the glomerular basement membrane. J. Ultrastruct. Res. 6, 19–27 (1962)

    Google Scholar 

  • Kurtz, S.M., McManus, J.F.A.: The fine structure of the human glomerular basement membrane. J. Ultrastruct. Res. 4, 81–87 (1960)

    Google Scholar 

  • Larra, F., Droz, B.: Techniques radioautographiques et leur application à l'étude du renouvellement des constituants cellulaires. J. Microscopic 9, 845–880 (1970)

    Google Scholar 

  • Leblond, C.P.: Personal communication (1973)

  • Luft, J.H.: Improvements in epoxy resin embedding methods. J. biophys. biochem. Cytol. 9, 409–414 (1961)

    Google Scholar 

  • Mahieu, P., Winand, R.J.: Chemical structure of tubular and glomerular basement membranes of human kidney. Isolation, purification, carbohydrate and amino-acid composition. Europ. J. Biochem. 12, 410–418 (1970)

    Google Scholar 

  • Pease, D.C.: Electron microscopy of the vascular bed of the kidney cortex. Anat. Rec. 121, 701–721 (1955)

    Google Scholar 

  • Sachot, N., Sternberg, M., Skwarlo, K., Rebeyrotte, P., Lagrue, G.: Comparison of isolation and chemical composition of kidney glomerular basement membranes in rabbit, rat and man. Comp. Biochem. Physiol. 50A, 575–579 (1975)

    Google Scholar 

  • Walker, F.: The origin, turnover and removal of glomerular basement membrane. J. Path. 110, 233–244 (1973)

    Google Scholar 

  • Wechsler, W.: Die Entwicklung der Gefäße und perivaskulären Gewebsräume im Zentralnervensystem von Hühnern. Elektronenmikroskopischer Beitrag zur Kenntnis der morphologischen Grundlagen der Blut-Hirn-Schranke während der Ontogenese. Z. Anat. Entwickl.-Gesch. 124, 367–395 (1965)

    Google Scholar 

  • Weinstock, M., Leblond, C.P.: Les étapes de la synthèse du collagène. Un. méd. Can. 101, 2079–2084 (1972)

    Google Scholar 

  • Westberg, N.G., Michael, A.F.: Human glomerular basement membrane. Preparation and composition. Biochem. 9, 3837–3846 (1970)

    Google Scholar 

Download references

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Authors and Affiliations

  1. Laboratoire de Neurobiologie Fonctionnelle, Université de Lille I, Villeneuve D'Ascq, France

    Pierre Delorme

  2. Laboratoire de Biologie Médicale et de Microscopic Electronique, Université de Nancy I, Nancy, France

    Georges Grignon

Authors
  1. Pierre Delorme
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  2. Georges Grignon
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Additional information

This work was supported by grants from CNRS (ERA 331, Laboratoire de Physiologie Générale I, Faculté des Sciences, Nancy I, 54037 — Nancy-Cedex, France) and from the Recherche Médicale Française

The authors are grateful to Dr. M. Mazzuca for helpful suggestions on the manuscript, to Mrs. Ch. Pelletier and Mrs. B. Coisne for secretarial assistance and to Miss. M.-Ch. Thuillier for technical assistance

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Delorme, P., Grignon, G. An autoradiographic study on the biosynthesis of the capillary basal lamina in the chick embryo telencephalon. Cell Tissue Res. 192, 299–308 (1978). https://doi.org/10.1007/BF00220747

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