Summary
Pancreatic polypeptide (PP) cells in the pancreas and gut of chickens were studied by immunocytochemistry. In the pancreas PP cells were numerous and disseminated in the exocrine parenchyma. In this location they were first seen at the 9th day of incubation, i.e. several days after the appearance of glucagon, insulin and somatostatin cells. Very large numbers of these cells occurred from about the 14th day until shortly after hatching when the PP cell frequency was somewhat reduced. At the 17th day of incubation PP cells appeared in the duodenum. Subsequently the number of PP cells in the duodenum increased, and PP cells began to appear also in the jejunum-ileum (19th day) and in the proventriculus and colon (21st day). At hatching and a few days thereafter, PP cells were relatively numerous in the small intestines but much less frequent in the proventriculus and colon. One week after hatching PP cells had disappeared from the colon but remained in the proventriculus and small intestines. Ultrastructurally the PP cell was clearly distinguishable from the insulin, glucagon and somatostatin cells. It was characterized by the presence of spherical cytoplasmic granules which were membrane-bound and moderately electron dense. Areas of firm adhesion between PP cells and acinar cells in the form of desmosomes and possibly also gap junctions were observed.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Adrian, T.E., Bloom, S.R., Bryant, M.G., Polak, J.M., Heitz, Ph., Barnes, A.J.: Distribution and release of human pancreatic polypeptide. Gut 17, 940–944 (1976)
Beaupain, D., Dieterlen-Lièvre, F.: Etude immunocytologique de la différenciation du pancréas endocrine chez l'embryon de Poulet. II. Glucagon. Gen. comp. Endocr. 23, 421–431 (1974)
Beauvillain, J.-C., Tramu, G., Dubois, M.P.: Characterization by different techniques of adrenocorticotropin and gondadotropin producing cells in Lerot pituitary (Eliomys quercinus). Cell Tiss. Res. 158, 301–317 (1975)
Björklund, A., Falck, B., Owman, Ch.: Fluorescence microscopic and microspectrofluorometric techniques for the cellular localization and characterization of biogenic amines. In: Methods of investigative and diagnostic endocrinology, edit. S.A. Berson, Vol. I: The thyroid and biogenic amines, edit, by J.E. Rall and F.J. Kopin, pp. 318–368. Amsterdam: North-Holland Publ. Comp. 1972
Dieterlen-Lièvre, F., Beaupain, D.: Etude immunocytologique de la différenciation du pancréas endocrine chez l'embryon de Poulet. I. Ilots à insuline. Gen. comp. Endocr. 22, 62–69 (1974)
Floyd, J.C., Fajans, S.S., Pek, S., Chance, R.E.: A newly recognized pancreatic polypeptide; plasma levels in health and disease. Recent. Progr. Hormone Res. 33, 519–570 (1977)
Greenberg, G.R., Mitznegg, P., Bloom, S.R.: Effect of pancreatic polypeptide on DNA synthesis in the pancreas. Experientia (Basel) 33, 1332–1333 (1977)
Hazelwood, R.L., Turner, S.D., Kimmel, J.R., Pollock, H.G.: Spectrum effects of a new polypeptide (third hormone?) isolated from the chicken pancreas. Gen. comp. Endocr. 21, 485–496 (1973)
Helmstaedter, V., Feurle, G.E., Forssmann, W.G.: Insulin-, glucagon-, and somatostatinimmunoreactive endocrine cells in the equine pancreas. Cell Tiss. Res. 172, 447–454 (1976)
Kimmel, J.R., Hayden, L.J., Pollock, H.G.: Isolation and characterization of a new pancreatic polypeptide hormone. J. biol. Chem. 250, 9369–9376 (1975)
Kimmel, J.R., Pollock, H.G., Hazelwood, R.L.: Isolation and characterization of chicken insulin. Endocrinol. 83, 1323–1330 (1968)
Kimmel, J.R., Pollock, H.G., Hazelwood, R.L.: A new polypeptide hormone. Fed. Proc. 30, 1318(Abs.) (1971)
Larsson, L.-I., Sundler, F., Håkanson, R.: Immunohistochemical localization of human pancreatic polypeptide (HPP) to a population of islet cells. Cell Tiss. Res. 156, 167–171 (1975)
Larsson, L.-I., Sundler, F., Håkanson, R.: Pancreatic polypeptide — a postulated new hormone: Identification of its cellular storage site by light and electron microscopic immunocytochemistry. Diabetologia 12, 211–226 (1976)
Larsson, L.-I., Sundler, F., Håkanson, R., Pollock, H.G., Kimmel, J.R.: Localization of APP, a postulated new hormone, to a pancreatic endocrine cell type. Histochemistry 42, 377–382 (1974)
Lin, T.-M., Chance, R.E.: Spectrum of gastrointestinal actions of a new bovine pancreas polypeptide (BPP). Gastroenterol. 62, 852 (Abs.) (1972)
Lin, T.-M., Chance, R.E.: Candidate hormones of the gut: Bovine pancreatic polypeptide (BPP) and avian pancreatic peptide (APP). Gastroenterol. 67, 737–739 (Abs.) (1974a)
Lin, T.-M., Chance, R.E.: Gastrointestinal actions of a new bovine pancreatic peptide (BPP). In: Endocrinology of the gut (eds. Chey and Brooks), pp. 143–145. New Jersey: C.B. Slack, Inc. (1974b)
Lin, T.-M., Chance, R.E., Evans, D.: Stimulatory and inhibitory actions of bovine pancreatic peptide (BPP) on gastric and pancreatic secretions of dogs. Gastroenterol. 64, 865 (Abs.) (1973)
Lin, T.-M., Evans, D.C., Chance, R.E.: Action of pancreatic polypeptide (BPP) on pancreatic secretion in dogs. Gastroenterol. 66, 852 (Abs.) (1974)
Lin, T.-M., Evans, D.C., Chance, R.E., Spray, G.F.: Bovine pancreatic peptide: Action on gastric and pancreatic secretion in dogs. Amer. J. Physiol. 232, 311–315 (1977)
Lundquist, I., Sundler, F., Ahrén, B., Alumets, J., Håkanson, R.: Somatostatin, pancreatic polypeptide, substance P and neurotensin. Cellular distribution and effects on stimulated insulin secretion in the mouse. Endocrinol., in press (1978)
Macerollo, P.C.: Pancreatic islet cells in very young chick embryos with special reference to D cells. Gen. comp. Endocr. 33, 139–146 (1977)
Malaisse-Lagae, F., Carpentier, J.-L., Patel, Y.C., Malaisse, W.J., Orci, L.: Pancreatic polypeptide: A possible role in the regulation of food intake in the mouse. Experientia (Basel) 33, 915–916 (1977)
Mayor, M.D., Hampton, J.C., Rosario, R.: A simple method for removing the resin from epoxyembedded tissue. J. biophys. Cytol. 9, 909–910 (1961)
Orci, L.: The microanatomy of the islets of Langerhans. Metabolism 25, Suppl. 1, 1303–1313 (1976)
Orci, L.L., Unger, R.H.: Functional subdivision of islets of Langerhans and possible role of D-cells. Lancet 1975, 1233–1244
Schwartz, T.W., Rehfeld, J.F., Stadil, F., Larsson, L.-I., Chance, R.E., Moon, N.: Pancreatic polypeptide response to food in duodenal-ulcer patients before and after vagotomy. Lancet 1976I, 1102–1105
Sundler, F., Håkanson, R., Larsson, L.-I.: Ontogeny of rat pancreatic polypeptide (PP) cells. Cell Tiss. Res. 178, 303–306 (1977a)
Sundler, F., Håkanson, R., Lundquist, I., Larsson, L.-I.: Effect of alloxan on rat pancreatic polypeptide (PP) cells. Cell Tiss. Res. 178, 307–312 (1977b)
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Alumets, J., Håkanson, R. & Sundler, F. Distribution, ontogeny and ultrastructure of pancreatic polypeptide (PP) cells in the pancreas and gut of the chicken. Cell Tissue Res. 194, 377–386 (1978). https://doi.org/10.1007/BF00236159
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00236159