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Ultrastructural localization of radiolabelled L-dopa in the endocrine hypothalamus of the rat

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Summary

Light and electron microscopic autoradiography has been employed to define the neuroanatomical patterns of uptake and binding of radiolabelled L-dopa in the endocrine hypothalamus of the rat. A dorsomedial continuum of arcuate and periventricular neurons selectively sequester 3H L-dopa 20 min following its intraventricular infusion. By 40 and 60 min following the infusion labelling of neurons is minimal and supports the notion of rapid degradation. Other cell compartments such as tanycytes demonstrate uptake of 3H L-dopa. The ultrastructural localization and distribution of radiolabelled L-dopa (or its metabolites) in the rodent hypothalamus is discussed with respect to mechanisms and cell compartments involved in neuroendocrine regulatory processes.

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Authors and Affiliations

  1. Department of Anatomy, University of Rochester, School of Medicine and Dentistry, 14642, Rochester, New York, USA

    David E. Scott, Phillip M. Scott & G. Krobisch-Dudley

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  1. David E. Scott
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  2. Phillip M. Scott
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  3. G. Krobisch-Dudley
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Supported by USPHS Program Project Grant NS-11642-04 (DES) and RR-05403

Career Development Awardee RO4GM-70001

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Scott, D.E., Scott, P.M. & Krobisch-Dudley, G. Ultrastructural localization of radiolabelled L-dopa in the endocrine hypothalamus of the rat. Cell Tissue Res. 195, 29–43 (1978). https://doi.org/10.1007/BF00233675

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