Summary
In the gastric mucosa of two teleost species, the perch (Perca fluviatilis) and the catfish (Ameiurus nebulosus) three endocrine cell types were found, located predominantly between the mucoid cells of the gastric mucosa. A fourth cell type is present in the gastric glands of catfish. Each cell type was defined by its characteristic secretory granules. Type-I cells were predominant in both fish. These cells contained round or oval granules with a pleomorphic core. The average diameter of granules was 400 nm for the perch and 270 nm for the catfish. Type-II cells of both species displayed small, highly osmiophilic granules about 100 nm in diameter. The secretory granules of type-III cells (260 nm in the perch and 190 nm in the catfish) were round or slightly oval in shape and were filled with a finely particulate electron-dense material. Type-IV cells of the catfish were found in the gastric glands only. Their cytoplasm was filled with homogeneous, moderately electron-dense granules averaging 340 nm in diameter. The physiological significance of these different morphological types of gastric endocrine cells requires further investigation.
Similar content being viewed by others
References
Alumets J, Sundler F, Håkanson R (1977) Distribution, ontogeny and ultrastructure of somatostatin immunoreactive cells in the pancreas and gut. Cell Tissue Res 185:465–479
Baetens D, Rufener C, Srikant BC, Dobbs R, Unger R, Orci L (1976) Identification of glucagon producing cells in dog gastric mucosa. J Cell Biol 69:455–464
Boquist L, Patent G (1971) The pancreatic islets of the teleost Scorpaena scropha. An ultrastructural study with particular regard to fibrillar granules. Z Zellforsch 115:416–425
Brinn JE (1975) Pancreatic islet cytology of Ictaluridae (Teleostei). Cell Tissue Res162:357–365
Buffa R, Capella C, Solcia E, Frigerio B, Said SI (1977) Vasoactive intestinal peptide (VIP) cells in the pancreas and gastrointestinal mucosa. Histochemistry 50: 217–227
Bussolati G, Pearse AGE (1970) Immunofluorescent localization of the gastrin-secreting G cells in the pyloric antrum of the pig. Histochemie 21:1–4
Capella C, Vassallo G, Solcia E (1971) Light and electron microscopic identification of histaminestoring argyrophil (ECL) cell in murine stomach and of its equivalent in other mammals. Z Zellforsch Mikrosk Anat 118:68–84
Carvalheira AF, Welsch U, Pearse AGE (1968) Cytochemical and ultrastructural observations on the argentaffin and argyrophil cells of gastrointestinal tract in mammals and their place in the APUD series of polypeptide-secreting cells. Histochemie 14:33–46
Dubois MP, Billard R, Breton B (1978) Use of immunofluorescence for localization of somatostatin-like antigen in the rainbow trout (Salmo gairdneri). Comparative distribution of LH-RF and neurophysin. Ann Biol Anim Biochem Biophys 18:843–851
Fänge R (1962) Pharmacology of poikilothermic vertebrates and invertebrates. Pharmacol Rev 14:281–316
Forssmann WG, Orci L (1969) Ultrastructure and secretory cycle of the gastrin-producing cell. Z Zellforsch 101:419–432
Forssmann WG, Orci L, Pictet R, Renolds AE, Rouiller CH (1969) The endocrine cells in the epithelium of the gastrointestinal mucosa of the rat. An electron microscopy study. J Cell Biol 40:692–715
Fritsch HAR, Sprang R (1977) On the ultrastructure of polypeptide hormone-producing cells in the gut of the Ascidian, Ciona intestinalis L. and the bivalve, Mytilus edulis L. Cell Tissue Res 177:407–413
Fujita T, Kobayashi S (1971) Experimental induced granule release in the enteroendocrine cells of dog pyloric antrum. Z Zellforsch 116:52–60
Gabe M, Martoja M (1971) Données histologiques sur les cellules endocrines gastriques et pancréatiques de Mugil auratus (Teleosteen, Mugiliforme). Arch Anat Microsc Morphol Exp 60:219–234
Gas N, Noaillac-Depeyre J (1978) Types cellulaires de l'estomac, assurant la production du suc gastrique, chez Ameiurus nebulosus L. Biol Cell 31:181–190
Greider MH, Steinberg V, Mc Guigan JE (1972) Electronmicroscopic identification of the gastrin cell of the human antral mucosa by means of immunocytochemistry. Gastroenterology 63:572–583
Heitz PH, Polak JM, Timson CM, Pearse AGE (1976) Enterochromaffin cells as the endocrine source of gastrointestinal substance P. Histochemistry 49:343–347
Helmstaedter V, Feurle GE, Forssmann WG (1977) Relationship of glucagon-somatostatin and gastrinsomatostatin cells in the stomach of the monkey. Cell Tissue Res 177:29–46
Johnson DE, Torrence JL, Elde RP, Bauer GE, Noe BD, Fletcher DJ (1976) Immunohistochemical localization of somatostatin, insulin and glucagon in the pancreatic islets of the anglerfish (Lophius americanus) and the channel catfish (Ictalurus punctata). Am J Anat 147:119–124
Katakoa K, Fujita H (1974) The occurrence of endocrine cells in the intestine of the Lancelet Branchiostoma japonicum. An electron microscope study. Arch Histol Jpn 36:401–406
Klein C (1977) Ultrastructural and cytochemical bases for the identification of cell types in the endocrine pancreas of teleosts. Intern Review of cytology Supplement 6 Acad Press 289–346
Klein C (1978) Use of immunocytochemical staining of somatostatin for correlative light and electron microscopic investigation of D cells in the pancreatic islet of Xiphophorus helleri H (Teleostei). Cell Tissue Res 194:399–404
Klein C, Van Noorden S (1980) Pancreatic polypeptide (PP) and glucagon cells in the pancreatic islet of Xiphophorus helleri H (Teleostei). Cell Tissue Res 205:187–198
Kobayashi K, Fujita T, Sasagawa T (1971) Electron microscope studies on the endocrine cells of the human gastric fundus. Arch Histol Jpn 32:429–444
Kobayashi K, Shibasaki S, Takahashi Y (1976) Light and electron microscopic study on the endocrine cells of the pancreas in a marine teleost, Fugu rubripes rubripes. Cell Tissue Res 174:161–182
Kudo S, Takahashi Y (1973) New cell types of the pancreatic islets in the crucian carp, Carassius carassius. Z Zellforsch 146:425–438
Langer M, Van Noorden S, Polak JM, Pearse AGE (1979) Peptide hormone-like immunoreactivity in the gastrointestinal tract and endocrine pancreas of eleven teleost species. Cell Tissue Res 199:493–508
Larsson LI, Rehfeld JF (1978) Evolution of C.C.K. like hormones. In: Bloom SR (ed) Gut hormones. Churchill Livingstone, New York, p 68–73
Larsson LI, Goltermann N, De Magistris L, Rehfeld JF, Schwartz T (1979) Somatostatin cell processes as pathways for paracrine secretion. Science 205:1393–1394
Lechago J, Weinstein WM (1978) Morphological aspects of the G-cells. In: Bloom SR (ed) Gut Hormones. Churchill Livingstone, New York, p 140–144
Ling EA, Tan CK (1975) Fine structure of the gastric epithelium of the coral fish, Chelmon rostratus Cuvier. Okajimas Folia Anat jap51:285–310
Lorentz W, Matejka E, Schmal A (1973) A phylogenic study on the occurrence and distribution of histamine in the gastrointestinal tract and other tissues of man and various animals. Comp Gen Pharmacol 4:229–250
Marx M, Schmidt W, Herrmann M, Goberna R (1970) Electron microscopic studies on the existence of so-called acinar-islet cells in the regenerating pancreas of the rat. Horm Metab Res 2:204–212
Melmed RN, Benitez CJ, Holt SJ (1972) Intermediate cells of the pancreas I: ultrastructural characterization. J Cell Sci 11:449–475
Mortensen NJMC, Morris JF (1977) The effect of fixation conditions on the ultrastructural appearance of gastric cell granules in the rat gastric pyloric antrum. Cell Tissue Res 176:251–263
Noaillac-Depeyre J, Gas N (1978) Ultrastructural and cytochemical study of the gastric epithelium in a fresh water teleostean fish (Perca fluviatilis). Tissue and Cell 10:23–37
Noaillac-Depeyre J, Hollande E (1981) Evidence for somatostatin, gastrin and pancreatic polypeptidelike substances in the mucosa cell of the gut in fishes with and without stomach. Cell Tissue Res 216:193–203
Pearse AGE, Takor Takor T (1976) Neuroendocrine embryology and the APUD concept. Clin Endocrinol 5 suppl 229s-244s
Pearse AGE, Coulling I, Weavers B, Friesen S (1970) The endocrine polypeptide cells of the human stomach, duodenum and jejunum. Gut 11:649–658
Polak JM, Pearse AGE, Heath CM (1975) Complete identification of endocrine cells in the gastrointestinal tract using semithin-sections to identify motilin cells in human and animal intestine. Gut 16:225–229
Read JB, Burnstock G (1968) Fluorescent histochemical studies on the mucosa of the vertebrate gastrointestinal tract. Histochemie 16:324–332
Reinecke M, Carraway RE, Falkmer S, Feurle GE, Forssmann WG (1980) Occurrence of neurotensin-immunoreactive cells in the digestive tract of lower vertebrates and deuterostomian invertebrates. A correlated immunohistochemical and radioimmunochemical study. Cell Tissue Res212:173–185
Rombout JHWM (1977) Enteroendocrine cells in the digestive tract of Barbus conchonius (Teleostei, Cyprinidae). Cell Tissue Res 185:435–450
Rombout JHWM, Rademakers LHPM, Vanhees JP (1979) Pancreatic endocrine cells of Barbus conchonius (Teleostei, Cyprinidae), and their relation to the enteroendocrine cells. Cell Tissue Res 203:9–23
Rubin W (1972) Endocrine cells in the normal human stomach. A fine structural study. Gastroenterology 63:784–800
Rubin W, Gershon MD, Ross LL (1971) Electron microscope radioautographic identification of serotonin-synthesizing cells in the mouse gastric mucosa. J Cell Biol 50:399–415
Rubin W, Schwartz B (1979) Electron microscopic radioautographic identification of the “enterochromaffin-like” APUD cells in murine oxyntic glands. Demonstration of a metabolic difference between rat and mouse gastric A-like cells. Gastroenterology 76:437–449
Solcia E, Vassallo G, Sampietro R (1967) Endocrine cells in the antropyloric mucosa of the stomach. Z Zellforsch 81:474–486
Solcia E, Pearse AGE, Grube D, Bussolati G, Ceutzfeldt W, Gepts W (1973) Revised Wiesbaden classification of gut endocrine cells. Rendic Gastroenterol 5:13–16
Stefan Y, Falmer S (1980) Identification of four endocrine cell types in the pancreas of Cottus scorpius (Teleostei) by immunofluorescence and electron microscopy. Gen Comp Endocrinol 42:171–178
Stefan Y, Dufour C, Falkmer S (1978) Mise en évidence par immunofluorescence de cellules à polypeptide pancréatique (PP) dans le pancréas et le tube digestif de poissons osseux et cartilagineux. CR Acad Sci 286:1073–1075
Stipp ACM, Ferri S, Sesso A (1980) Fine structural analysis of a teleost exocrine pancreas cellular components. A freeze fracture and transmission electron microscopic study. Anat Anz 147:60–75
Sundler F, Alumets J, Holst J, Larsson LI, Håkanson R (1976) Ultrastructural identification of cells storing pancreatic-type glucagon in dog stomach. Histochemistry 50:33–37
Thomas NW (1970) Morphology of endocrine cells in the islet tissue of the cod Gadus callarias. Acta Endocrinol 63:679–695
Thorndyke MC, Bevis PJR (1978) Endocrine cells in the gut of the Ascidian Styela clava. Cell Tissue Res 187:159–165
Van Noorden S, Pearse AGE (1974) Immunoreactive polypeptide hormones in the pancreas and gut of the lamprey. Gen Comp Endocrinol 23:311–324
Vassallo G, Capella C, Solcia E (1971) Endocrine cells of the human gastric mucosa. Z Zellforsch 118:49–67
Venable JH, Coggeshall R (1965) A simplified lead citrate stain for use in electron microscopy. J Cell Biol 25:405–408
Watari N, Tsukagoshi N, Honna Y (1970) The correlative light and electron microscopy of the islets of Langerhans of some lower vertebrates. Arch Histol Jap 31:371–392
Watson AHD (1979) Fluorescent histochemistry of the teleost gut: Evidence for the presence of serotonergic neurones. Cell Tissue Res 197:155–164
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Noaillac-Depeyre, J., Gas, N. Ultrastructure of endocrine cells in the stomach of two teleost fish Perca fluviatilis L. and Ameiurus nebulosus L.. Cell Tissue Res. 221, 657–678 (1982). https://doi.org/10.1007/BF00215709
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00215709