Skip to main content
SpringerLink
Log in

Vasoactive intestinal polypeptide (VIP) immunoreactivity of endocrine-like cells in the feline pyloric mucosa

  • Published:
Cell and Tissue Research Aims and scope Submit manuscript

Summary

Immunoreactivity to VIP by endocrine-like cells in the feline pyloric mucosa was examined by using three kinds of region-specific anti-porcine VIP sera. VIP-immunoreactive endocrine-like cells were detected clearly with all of the VIP antisera used. They were located mainly around the neck of the pyloric glands. Some of these endocrine-like cells showed dilution-dependent immunoreactivity against VIP antisera. The immunostaining intensity of VIP-immunoreactive endocrine-like cells showing dilution-independence could not be distinguished from those of nerve elements. The present results suggest that the immunoreactivity with properties very similar to those of authentic VIP may be present in the endocrine-like cells of the feline pyloric glands.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Bryant MG, Bloom SR, Polak JM, Albuquerque RH, Modlin IM, Pearse AGE (1976) Possible dual role for vasoactive intestinal peptide as gastrointestinal hormone and neurotransmitter substance. Lancet 1:991–993

    Google Scholar 

  • Buffa R, Capella C, Solcia E, Frigerio B, Said SI (1977) Vasoactive intestinal peptide (VIP) cells in the pancreas and gastrointestinal mucosa. Histochemistry 50:217–227

    Google Scholar 

  • Buffa R, Capella C, Fontana P, Usellini L, Solcia E (1978) Type of endocrine cells in the human colon and rectum. Cell Tissue Res 192:227–240

    Google Scholar 

  • Chayvialle JA, Miyata M, Rayford PL, Thompson JC (1980) Immunoreactive somatostatin and vasoactive intestinal peptide in the digestive tract of cats. Gastroenterol 79:837–843

    Google Scholar 

  • Diamaline R, Dockray GJ (1978) Multiple immunoreactive forms of vasoactive intestinal polypeptide in human colonic mucosa. Gastroenterolol 75:217–227

    Google Scholar 

  • Fahrenkrug J (1979) Vasoactive intestinal polypeptide: measurement, distribution and putative neurotransmitter function. Digestion 19:149–169

    CAS  PubMed  Google Scholar 

  • Grube D (1980) Immunoreactivities of gastrin (G-) cells. II. Non-specific binding of immunoglobulins to G-cells by ionic interactions. Histochemistry 66:149–167

    Google Scholar 

  • Larsson LI, Fahrenkrug J, Schaffalitzky de Muckadell OB, Sundler F, Håkanson R, Rehfeld JF (1976) Localization of vasoactive intestinal polypeptide (VIP) to central and peripheral neurons. Proc Natl Acad Sci USA 73:3179–3200

    Google Scholar 

  • Larsson LI, Polak JM, Buffa R, Sundler F, Solcia E (1979) Immunocytochemical localization of the vasoactive intestinal polypeptide. J Histochem Cytochem 27:936–938

    Google Scholar 

  • Polak JM, Pearse AGE, Garoud JC, Bloom SR (1974) Cellular localization of a vasoactive intestinal peptide in the mammalian and avian gastrointestinal tract. Gut 15:720–724

    Google Scholar 

  • Said SI (1979) Vasoactive intestinal polypeptide (VIP) as a neural peptide. In: Miyoshi A (ed) Gut peptide. Kodansha, Ltd, Tokyo, Elsevier North-Holland, Biomedical Press, Amsterdam New York Oxford, pp 268–274

    Google Scholar 

  • Said SI, Mutt V (1970) Polypeptide with broad biological activity: Isolation from small intestine. Science 169: 1217–1218

    Google Scholar 

  • Said SI, Mutt V (1972) Isolation from porcine-intestinal wall of a vasoactive octacosa peptide related to secretin and to glucagon. Eur J Biochem 28:199–204

    Google Scholar 

  • Solcia E, Capella C, Vassallo G, Buffa R (1975) Endocrine cells of the gastric mucosa. Internat Rev Cytol 42:223–286

    Google Scholar 

  • Sternberger LA (1979) Immunocytochemistry 2nd ed. Prentice-Hall Inc, Englewood Cliffs, New Jersey

    Google Scholar 

  • Sundler F, Alumets J, Håkanson R, Fahrenkrug J, Schaffalitzky de Muckadell OB (1978) Peptidergic (VIP) nerves in the pancreas. Histochemistry 55:173–176

    Google Scholar 

  • Sundler F, Alumets J, Håkanson R, Fahrenkrug J, Schaffalitzky de Muckadell OB (1979) Cellular localization and ontogeny of immunoreactive vasoactive intestinal polypeptide (VIP) in the chicken gut. Cell Tissue Res 196:193–201

    Google Scholar 

  • Yanaihara C, Sakagami M, Mochizuki T, Sato H, Yanaihara N, Iwanaga T, Fujii S, Fujita T (1980) Immunoreactive VIP (vasoactive intestinal polypeptide) in canine intestinal mucosa and muscle. Biomed Res 1:449–455

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Veterinary Anatomy, Obihiro University of Agriculture and Veterinary Medicine, Obihiro

    J. Yamada, N. Kitamura, T. Yamashita & M. Misu

  2. Laboratory of Bioorganic Chemistry, Shizuoka College of Pharmacy, Shizuoka, Japan

    N. Yanaihara

Authors
  1. J. Yamada
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. N. Kitamura
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. T. Yamashita
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. M. Misu
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. N. Yanaihara
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Yamada, J., Kitamura, N., Yamashita, T. et al. Vasoactive intestinal polypeptide (VIP) immunoreactivity of endocrine-like cells in the feline pyloric mucosa. Cell Tissue Res. 226, 113–120 (1982). https://doi.org/10.1007/BF00217086

Download citation

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00217086

Key words

Search

Navigation