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Regeneration of splenic tissue after autologous subcutaneous implantation: Homing of T- and B- and Ia-positive cells in the white pulp of the rat spleen

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Summary

The localization of T-and B lymphocytes and interdigitating cells (IDC) was investigated during the regeneration process of splenic implants. For this purpose a two-step immunoperoxidase technique was used to visualize T-cell antigen, immunoglobulins and Ia-antigen on cryostat sections. The specific localization of the repopulating lymphocytes occurred simultaneously with the development of non-lymphoid elements characteristic for the different compartments of the white pulp, i.e., the periarteriolar lymphocyte sheaths (PALS) and follicles. The marginal zone (MZ) developed after the PALS and primary follicles, but before germinal center reactions were found. During ontogeny, however, the development of a broad MZ precedes the formation of follicles. This difference in sequence of events is discussed.

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Abbreviations

PALS:

periarteriolar lymphocyte sheaths

MZ:

marginal zones

IDC:

interdigitating cells

FDC:

follicular dendritic cells

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Authors and Affiliations

  1. Department of Histology, Medical Faculty, Free University, Amsterdam, The Netherlands

    Christine D. Dijkstra, E. A. Döpp & H. L. Langevoort

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  1. Christine D. Dijkstra
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  2. E. A. Döpp
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  3. H. L. Langevoort
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Dijkstra, C.D., Döpp, E.A. & Langevoort, H.L. Regeneration of splenic tissue after autologous subcutaneous implantation: Homing of T- and B- and Ia-positive cells in the white pulp of the rat spleen. Cell Tissue Res. 229, 97–107 (1983). https://doi.org/10.1007/BF00217883

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