Summary
Freshly dispersed aortic smooth muscle cells of the rat were grown in either 5% or 20% oxygen. Cells proliferated more rapidly in 5% than in 20% oxygen although at confluency the protein content per cell was 20% less in the 5% than in the 20% oxygen environment. By electron microscopy, cell morphology was the same in both environments. Uptake of low-density and high-density homologous lipoprotein was unaffected by oxygen tension. Other studies, however, showed that induction of receptors for lipoprotein binding behaved variably in the low compared to the high oxygen condition. These differences were not associated with differences in lipid synthesis in the two conditions.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Adebonojo FO, Bensch LG, King DW (1961) The effect of nitrogen on the enzymatic pattern of strain L cells. Cancer Res 21:252–256
Albers JJ, Bierman EL (1976) The effect of hypoxia on uptake and degradation of low density lipoproteins by cultured human arterial smooth muscle cells. Biochim Biophys Acta 424:422–429
Bierman EL, Albers J (1977) Regulation of low density lipoprotein receptor activity by cultured human arterial smooth muscle cells. Biochim Biophys Acta 488:152–160
Bilheimer DW, Eisenberg S, Levey RI (1972) The metabolism of very low density lipoprotein proteins I. Preliminary in vitro and in vivo observations. Biochim Biophys Acta 260:212–221
Brown MS, Goldstein JL (1974) Familial hypercholesterolemia: defective binding of lipoproteins to cultured fibroblasts associated with impaired regulation of 3-hydroxy-3-methylglutaryl coenzyme A reductase activity. Proc Natl Acad Sci USA 71:788–792
Burton K (1956) A study of the conditions and mechanisms of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid. Biochem J 62:315–323
Chamley JH, Campbell GR, McConnell JD, Gröschel-Stewart U (1977) Comparison of vascular smooth muscle cells from adult human, monkey and rabbit in primary culture and in subculture. Cell Tissue Res 177:503–522
Chamley-Campbell J, Campbell GR, Ross R (1979) The smooth muscle cell in culture. Physiol Rev 59:1–61
Giles KW, Mayers A (1965) An improved diphenylamine method for the estimation of deoxyribonucleic acid. Nature 206:93
Goldstein JL, Basu SK, Brunschede GY, Brown MS (1976) Release of low density lipoprotein from its cell surface receptor by sulfated glycosaminoglycans. Cell 7:85–95
Gordon GB, Barcza MA, Bush ME (1977) Lipid accumulation in hypoxic tissue culture cells. Am JPathol 88:663–674
Havel RJ, Eder HA, Bragdon JH (1955) The distribution and chemical components of ultracentrifugally separated lipoproteins in human serum. J Clin Invest 34:1345–1353
Helin P, Lorenzen I (1964) Arteriosclerosis in rabbit aorta induced by systemic hypoxia. Angiology 20:1–12
Hollenberg M (1971) Effect of oxygen on growth of cultured myocardial cells. Circ Res 28:148–157
Lippiello PM, Holloway CT, Garfield SA, Holloway PW (1979) The effect of estradiol on stearyl-CoA desaturase activity and microsomal membrane properties in rooster liver. J Biol Chem 254:2004–2009
Mahley RW, Innerarity TC, Weisgraber KH, Fry DL (1977) Canine hyperlipoproteinemia and atherosclerosis. Am J Pathol 87:226–250
May J, Paule WJ, Rounds DG, Blankenhorn DH, Zemplenyi T (1975) The introduction of atherosclerotic plaque-like mounds in cultures of aortic smooth muscle cells. Virchows Arch B Cell Path 18:205–211
Niinikowski J, Heughan C, Hunt TK (1973) Oxygen tensions in the aortic wall of normal rabbits. Atherosclerosis 17:353–359
Paule WJ, Zemplenyi TK, Rounds DE, Blankenhorn DH (1976) Light-and-electron microscopic characteristics of arterial smooth muscle cell cultures subjected to hypoxia or carbon monoxide. Atherosclerosis 25:111–123
Robertson AL, Khairallah PA (1973) Arterial endothelial permeability and vascular disease. Exp Mol Pathol 18:241–260
Ross R (1971) The smooth muscle cell: II. Growth of smooth muscle in culture and formation of elastic fibers. J Cell Biol 50:172–186
Rudel LL, Lee JA, Morris MD, Felts JM (1974) Characterization of plasma lipoproteins separated and purified by agarose-column chromatography. Biochem J 139:89–95
Stein O, Stein Y (1975) Surface binding and interiorization of homologous and heterologous serum lipoproteins by rat aortic smooth muscle cells in culture. Biochim Biophys Acta 398:377–384
Udenfriend S (1972) Fluorescamine: a reagent for assay of amino acids, peptides, proteins and primary amines in the picomole range. Science 178:871–872
Weinstein DB, Carew TE, Steinberg DL (1976) Uptake and degradation of low density lipoprotein by swine arterial smooth muscle cells with inhibition of cholesterol biosynthesis. Biochim Biophys Acta 424:404–421
Author information
Authors and Affiliations
Additional information
Funded by USPHS 1-P-1 HL19242
Rights and permissions
About this article
Cite this article
Kuehl, K.S., Holloway, P.W. Lipoprotein uptake by vascular smooth muscle cells of the rat cultured in 5% or 20% oxygen. Cell Tissue Res. 233, 453–462 (1983). https://doi.org/10.1007/BF00238310
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00238310