Summary
The growth-promoting effect of caerulein on antral gastric mucosa was explored using Wistar rats. Implanted osmotic minipumps were used to administer submaximal doses of either caerulein or saline to normal rats for up to 4 days. In one group, reflux of bile and pancreatic juice into the stomach was avoided by previous surgical diversion of the distal common bile duct to the jejunum. DNA synthetic and mitotic activity in the antrum epithelium were estimated by 3H-thymidine pulse labelling and autoradiography during the administration of the peptide. The rate of cell migration was determined in animals killed 1, 2 and 3 days after the 3H-thymidine pulse. Administration of caerulein to normal rats provoked significant increases in both labelling and mitotic indices, and a significant acceleration of the upward cell migration in the glandular tubes. In the animals with distal diversion of bile and pancreatic secretions both labelling and mitotic indices were also increased over control values under the effect of the peptide. These data indicate that administration of caerulein stimulates cell proliferation in the antral gastric mucosa. This effect cannot be explained through increased reflux of pancreaticobiliary secretions in the stomach.
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References
Barrowman JA, Mayston PD (1974) The trophic influence of cholecystokinin on the rat pancreas. J Physiol 238:73–75
Brants F, Morisset J (1976) Trophic effect of cholecystokinin-pan-creozymin on pancreatic acinar cells from rats of different ages. Proc Soc Exptl Biol Med 153:523–527
Caes F, Lamote J, Willems G (1983) Effect of caerulein on epithelil cell proliferation in the murine stomach. Hepatogastroenterology 30:63–65
Casteleyn PP, Dubrasquet M, Willems G (1977) Opposite effects of gastrin on cell proliferation in the antrum and other parts of the upper gastrointestinal tract in the rat. Am J Dig Dis 22:798–804
Chey WY, Sivasomboon B, Hendricks J, Lorber SH (1970) Effects of secretin and cholecystokinin on gastric secretion in rats. Gastroenterology 58:1037
Crean GP, Hogg DF, Rumsey RD (1969) Hyperplasia of the gastric mucosa produced by duodenal obstruction. Gastroenterology 56:193–199
as HT, Farooq O, Grossman MI (1975) Inhibition of gastric emptying is a physiological action of cholecystokinin. Gastroenterology 58:1211–1217
Dembinsky AB, Johnson LR (1980) Stimulation of pancreatic growth by secretin, caerulein and pentagastrin. Endocrinology 106:323–328
Fölsch UR, Winckler K, Wormsley KG (1978) Influence of repeated administration of cholecystokinin and secretin on the pancreas of the rat. Scand J Gastroenterol 13:663–671
Hart-Hansen O, Pedersen T, Larssen JK, Rehfeld JF (1976) Effect of gastrin on gastric mucosal cell proliferation in man. Gut 17:536–541
Hughes CA, Bates T, Dowling RH (1978) Cholecystokinin and secretin prevent the intestinal mucosal hypoplasia of total parenteral nutrition in the dog. Gastroenterology 75:34–41
Johnson LR, Guthrie PD (1976) Effect of cholecystokinin and 16, 16-dimethyl PgE2 on RNA and DNA of gastric and duodenal mucosa. Gastroenterology 70:59–65
Lamote J, Putz P, Willems G (1982) Effect of cholecystokininoctapeptide, caerulein, and pentagastrin on epithelial cell proliferation in the murine gallbladder. Gastroenterology 83:371–377
Lehy T, Dubrasquet M, Bonfils S (1979) Effect of somatostatin on normal and gastrin-stimulated cell proliferation in the gastric and intestinal mucosae of the rat. Digestion 19:99–109
Mainz DL, Black O, Webster PD (1973) Hormonal control of pancreatic growth. J Clin Invest 52:2300–2304
Miller LR, Jacobson ED, Johnson LR (1973) Effect of pentagastrin on gastric mucosal cells grown in tissue culture. Gastroenterology 64:254–267
Morisset J, Génik P (1983) Effects of acute and chronic administration of secretin and caerulein on rat duodenal and gastric growth. Regul Pept 5:111–123
Sawicki W, Blaton O, Rowinski J (1971) Correction of autoradiographic grain count in respect to precisely calculated background. Histochemie 26:67–73
Solomon TE (1981) Regulation of exocrine pancreatic cell proliferation and enzyme synthesis. In: Physiology of the gastrointestinal tract. Raven Press, New York, pp 873–892
Solomon TE, Petersen H, Elashoff J, Grossman MI (1978) Interaction of caerulein and secretin on pancreatic size and composition in rat. Am J Physiol 235 (6):E174-E719
Weser E, Bell D, Tawil T (1981) Effects of octapeptidecholecystokinin, secretin, and glucagon on intestinal mucosal growth in parenterally nourished rats. Dig Dis Sci 25 (5): 409–416
Willems G (1981) Cellular kinetics and gastric mucosal disease. In: Domschke W, Wormsley KG (eds) Magen und Magenkrankheiten. Georg Thieme, Stuttgart New York, pp 195–204
Willems G, Lehy T (1975) Radioautographic and quantitative studies on parietal and peptic cell kinetics in the mouse. Gastroenterology 69:416–426
Willems G, Vansteenkiste Y (1974) Resistance of the antral mucosa to the trophic effect of gastrin in the dog. Biol Gastroenterol (Paris) 7:237–240
Willems G, Vansteenkiste Y, Limbosch JM (1972) Stimulating effect of gastrin on cell proliferation kinetics in canine fundic mucosa. Gastroenterology 62:583–589
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Caes, F., Willems, G. Administration of caerulein to rats promotes antral epithelial cell renewal. Cell Tissue Res. 236, 711–715 (1984). https://doi.org/10.1007/BF00217242
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DOI: https://doi.org/10.1007/BF00217242