Summary
An electron microscopic examination of human fetal ovaries reveals the presence of intercellular bridges between developing germ cells. The bridges are characterized by a band of electron-dense material beneath the lateral limiting membrane, and cell organelles are seen within the confines of these connections. Their general morphology is similar to that described in ovaries of other species. The possible functional significance of these connections is discussed.
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Anderson, E., Huebner, E.: Development of the oocyte and its accessory cell of the polychaete, Diopatra cuprea (Bosc). J. Morph. 126, 163–198 (1968).
Baca, M., Zamboni, L.: The fine structure of human follicular oocytes. J. Ultrastruct. Res. 19, 354–381 (1967).
Baker, T. G.: A quantitative and cytological study of germ cells in human ovaries. Proc. roy. Soc. B 158, 417–433 (1963).
—, Franchi, L. L.: The fine structure of oogonia and oocytes in human ovaries. J. Cell Sci. 2, 213–224 (1967).
Burgos, M. H., Fawcett, D. W.: Studies on the fine structure of the mammalian testis. I. Differentiation of the spermatids in the cat. J. biophys. biochem. Cytol. 1, 287–300 (1955).
Fawcett, D. W., Ito, S., Slautterback, D. L.: The occurrence of intercellular bridges in groups of cells exhibiting synchronous differentiation. J. biophys. biochem. Cytol. 5, 453–460 (1959).
Franchi, L. L., Mandl, A. M.: The ultrastructure of oogonia and oocytes in the fetal and neonatal rat. Proc. roy. Soc. B 157, 99–114 (1962).
Freeman, J. A., Spurlock, B. O.: A new epoxy embedment for electron microscopy. J. Cell Biol. 13, 437–443 (1962).
Gondos, B., Zamboni, L.: Ovarian development: The functional importance of germ cell interconnections. Fertil. and Steril. 20, 176–189 (1969).
Hertig, A. T., Adams, E. C.: Studies on the human oocyte and its follicle. I. Ultrastructural and histochemical observations on the primordial follicle stage. J. Cell Biol. 34, 647–675 (1967).
Karlsson, U., Schultz, R. L.: Fixation of the central nervous system for electron microscopy by aldehyde perfusion. J. Ultrastruct. Res. 12, 160–186 (1965).
King, R. C., Aggarwal, S. K., Aggarwal, U.: The development of the female Drosophila reproductive system. J. Morph. 124, 143–166 (1968).
Koch, E. A., King, R. C.: Further studies on the ring canal system of the ovarian cystocytes of Drosophila melanogaster. Z. Zellforsch. 102, 129–152 (1969).
Nagano, T.: The structure of cytoplasmic bridges in dividing spermatocytes of the rooster. Anat. Rec. 141, 73–79 (1961).
Odor, D. L., Blandau, R. J.: Ultrastructural studies on fetal and early postnatal mouse ovaries. II. Cytodifferentiation. Amer. J. Anat. 125, 177–215 (1969).
Ruby, J. R., Dyer, R. F., Skalko, R. G.: The occurrence of intercellular bridges during oogenesis in the mouse. J. Morph. 127, 307–340 (1969).
Volpe, E. P.: Intercellular bridges between germ cells in the developing ovary of the tadpole, Rana pipiens. (In Press, 1970).
Stefanini, M., DeMartino, C., Zamboni, L.: Fixation of ejaculated spermatozoa for electron microscopy. Nature (Lond.) 216, 173–174 (1967).
Stegner, H.-E.: Die elektronenmikroskopische Struktur der Eizelle. Ergebn. Anat. Entwickl.-Gesch. 39, 1–112 (1967).
—, Wartenberg, H.: Elektronenmikroskopische Untersuchungen an Eizellen des Menschen in verschiedenen Stadien der Oogenese. Arch. Gynäk. 199, 151–172 (1963).
Streeter, G. L.: Weight, sitting height, head size, foot length and menstrual age of the human embryo. Contr. Embryol. Carneg. Instn. 11, 143–170 (1920).
Venable, J. H., Coggeshall, R.: A simplified lead citrate stain for use in electron microscopy. J. Cell Biol. 25, 407–408 (1965).
Wagenen, G. van, Simpson, M. E.: Embryology of the ovary and testes Homo sapiens and Macaca mulatta. New Haven and London: Yale University Press 1965.
Watson, M. L.: Staining of tissue sections for electron microscopy with heavy metals. J. biophys. biochem. Cytol. 4, 475–478 (1958).
Weakley, B. S.: Electron microscopy of the oocyte and granulosa cells in developing ovarian follicles of the golden hamster (Mesocricetus auratus). J. Anat. (Lond.) 100, 503–534 (1966).
—: Light and electron microscopy of developing germ cells and follicle cells in the ovary of the golden hamster: twenty-four hours before birth to eight days post partum. J. Anat. (Lond.) 101, 435–459 (1967).
Witschi, E.: Development of vertebrates, p. 40–47. Philadelphia: Saunders 1956.
Zamboni, L., Gondos, B.: Intercellular bridges and synchronization of germ cell differentiation during oogenesis in the rabbit. J. Cell Biol. 36, 276–282 (1968).
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This work was supported by grants from the Edward G. Schlieder Educational Foundation, New Orleans, Louisiana State University and HD-03288 from the National Institute of Child Health and Human Development.
The authors would like to thank Miss Cathy Chase for her technical assistance.
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Ruby, J.R., Dyer, R.F., Gasser, R.F. et al. Intercellular connections between germ cells in the developing human ovary. Z. Zellforsch. 105, 252–258 (1970). https://doi.org/10.1007/BF00335475
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DOI: https://doi.org/10.1007/BF00335475