Summary
Magnocellular neurones in the supraoptic nucleus of the homozygous Brattleboro rat, which are unable to produce vasopressin, were investigated by immunocytochemistry to identify both the oxytocin cells and the abnormal neurones, which in normal animals would produce vasopressin. The abnormal cell profiles were significantly more rounded than those of the oxytocin cells. Both cell types showed evidence of hyperactivity, but the Golgi apparatus was more extensive in the oxytocin cells, probably as a result of the failure of the abnormal cells to produce vasopressin and its neurophysin and the resultant reduction in hormone packaging. Neurosecretory granules (NSG) 160 nm in diameter were found in the oxytocin perikarya but were absent from the abnormal cell bodies. In addition, a population of small dense granules (SDG) 100 nm in diameter was observed in both types of neurone, in numbers equal to the NSG in oxytocin cells.
Injection of a low, non-lethal dose of the axonal transport inhibitor colchicine resulted in a rapid and equal accumulation of both NSG and SDG in oxytocin perikarya and of SDG in the abnormal perikarya after one day. The effects of colchicine were reversed 2–3 days after administration. The SDG, which may contain a co-transmitter or co-hormone substance, are thus produced at a similar rate to NSG, and appear to be transported from the perikarya for subsequent release at the nerve endings.
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References
Boudier J-A, Detieux Y, Dutillet B, Cau P (1971) Effets d'injections intra-cisternales de colchicine sur la neurohypophyse du rat réhydraté après privation d'eau. C R Soc Biol 165:1089–1093
Boudier J-A, Detieux Y, Dutillet B, Luciani J (1972) Effets de la colchicine sur le noyau supraoptique du Rat réhydraté après privation d'eau. C R Acad Sci 274:1051–1054
Cannata MA, Morris JF (1973) Changes in the appearance of hypothalamo-neurohypophysial neurosecretory granules associated with their maturation. J Endocrinol 57:531–538
Chapman DB, Morris JF, Valtin H 1985 Granule and hormone accumulation and neuronal plasticity in the neural lobe of Brattleboro rats resulting from correction of their hereditary diabetes insipidus (Submitted)
Dellmann H-D, Sikora-Vanmeter KC (1982) Reversible fine structural changes in the supraoptic nucleus of the rat following intraventricular administration of colchicine. Brain Res Bull 8:171–182
Flament-Durand J, Dustin P (1972) Studies on the transport of secretory granules in the magnocellular hypothalamic neurone. 1. Action of colchicine on axonal flow and neurotubules in the paraventricular nuclei. Z Zellforsch 130:440–454
Flament-Durand J, Couck A-M, Dustin P (1975) Studies on the transport of secretory granules in the magnocellular hypotha- lamic neurons of the rat. Cell Tissue Res 164:1–9
Fuxe K, Agnati LF, Ganten D, Lang RE, Calza L, Poulsen K, Infantellina F (1982) Morphometric evaluation of the coexistence of renin-like and oxytocin-like immunoreactivity in nerve cells of the paraventricular hypothalamic nucleus of the rat. Neurosci Lett 33:19–24
Gainer H, Loh YP, Same Y (1977) Biosynthesis of neuronal peptides. In: Gainer H (ed) Peptides in neurobiology. Plenum Press, New York and London, pp 183–219
Hindelang-Gertner C, Stoeckel M-E, Porte A, Stutinsky F (1976) Colchicine effects in neurosecretory neurons and other hypo- thalamic and hypophysial cells, with special reference to chan- ges in cytoplasmic membranes. Cell Tissue Res 170:17–41
Martin R, Voigt KV (1981) Enkephalins co-exist with oxytocin and vasopressin in nerve terminals of rat neurohypophysis. Nature (London) 289:502–504
Martin R, Geis R, Holl R, Schafer M, Voigt KV (1983) Co-existence of unrelated peptides in oxytocin and vasopressin terminals of rat neurohypophyses: Immunoreactive methionine5-enkephalin-, leucine5-enkephalin- and cholecystokinin-like substances. Neuroscience 8:213–227
Morris JF (1976) Hormone storage in individual neurosecretory granules of the pituitary gland: A quantitative ultrastructural approach to hormone storage in the neural lobe. J Endocrinol 68:209–224
Morris JF (1982) The Brattleboro magnocellular neurosecretory system: A model for the study of peptidergic neurons. Ann NY Acad Sci 394:54–69
Morris JF, Nordmann JJ (1982) Membrane retrieval by vacuoles after exocytosis in the neural lobe of Brattleboro rats. Neuroscience 7:1631–1639
Morris JF, Sokol HW, Valtin H (1977) One neuron-one hormone? Recent evidence from Brattleboro rats. In: Moses A, Share L (eds) Neurohypophysis, Int Confn Key Biscayne Fla. Karger Basel, pp 58–66
Morris JF, Nordmann JJ, Dyball REJ (1978) Structure-function correlation in mammalian neurosecretion. Int Rev Exp Pathol 18:1–95
Nörstrom A, Hansson H-A, Sjöstrand J (1971) Effects of colchicine on axonal transport and ultrastructure of the hypothalamo- neurohypophyseal system of the rat. Z Zellforsch 113:271–293
Orkand PM, Palay SL (1966) The fine structure of the supraoptic nucleus in normal rats compared with that in rats with hereditary diabetes insipidus. Anat Rec 154:396
Palay SL (1955) An electron microscope study of the neurohypophysis in normal, hydrated and dehydrated rats. Anat Rec 121:348
Parish DC, Rodríguez EM, Birkett SD, Pickering BT (1981) Effects of small doses of colchicine on the compartments of the hypothalamo -neurohypophysial system of the rat. Cell Tissue Res 220:809–827
Pickering BT, Parish DC, Rodríguez EM, Gonzalez CB, Birkett S, Swann RW (1981) The movement of secretory product in the hypothalamo-neurohypophysial neurone. Verh Anat Ges 75:985–987
Polak JM, Pearse AGE, Heath CM (1975) Complete identification of endocrine cells in the gastrointestinal tract using semi-thin sections to identify motilin cells in the human and animal intes- tine. Gut 16:225–229
Schmale H, Richter D (1984) Single base deletion in the vasopressin gene is the cause of diabetes insipidus in Brattleboro rats. Nature 308:705–709
Sherlock DA, Field PM, Raisman G (1975) Retrograde transport of horseradish peroxidase in the magnocellular neurosecretory system of the rat. Brain Res 88:403–414
Silverman AJ, Zimmerman EA (1975) Ultrastructural immunocytochemical localization of neurophysin and vasopressin in the median eminence and posterior pituitary of the guinea pig. Cell Tissue Res 159:291–301
Sofroniew MV (1983) Morphology of vasopressin and oxytocin neurones and their central and vascular projections. Prog Brain Res 60:101–114
Sofroniew MV, Schrell U (1982) Long term storage and regular repeated use of diluted antisera in glass staining jars for increased sensitivity, reproducibility, and convenience of singleand two — color light microscopic immunocytochemistry. J Histochem Cytochem 30:504–511
Sofroniew MV, Weindl A, Schinko I, Wetzstein R (1979) The distribution of vasopressin-, oxytocin-, and neurophysin-producing neurons in the guinea pig brain. Cell Tissue Res 196:367–384
Sokol HW, Valtin H (1965) Morphology of the neurosecretory system in rats homozygous and heterozygous for hypothalamic diabetes insipidus (Brattleboro strain). Endocrinology 77:692–700
Stoeckel ME, Porte E, Klein MJ, Cuello AC (1982) Immunocytochemical localization of substance P in the neurohypophysis and hypothalamus of the mouse compared with the distribution of other neuropeptides. Cell Tissue Res 223:533–544
Sunde DA, Sokol HW (1975) Quantification of rat neurophysins by polyacrylamide gel electrophoresis (PAGE): application to the rat with hereditary hypothalamic diabetes insipidus. Ann NY Acad Sci 248:345–364
Swanson LW, Sawchenko PE (1980) Paraventricular nucleus: A site for the integration of neuroendocrine and autonomic mech- anisms. Neuroendocrinology 31:410–417
Valtin H (1982) The discovery of the Brattleboro rat, Recommended nomenclature, and the question of proper controls. Ann NY Acad Sci 394:1–9
Valtin H, Sawyer WH, Sokol HW (1965) Neurohypophysial principles in rats homozygous and heterozygous for hypothalamic diabetes insipidus (Brattleboro strain). Endocrinology 77:701–706
Vanderhaegen JJ, Lotstra F, Vandesande F, Dierickx K (1981) Coexistence of cholecystokinin and oxytocin-neurophysin in some magnocellular hypothalamo-hypophyseal neurons. Cell Tissue Res 221:227–231
Vandesande F, Dierickx K (1976) Immuno-cytochemical demonstration of the inability of the homozygous Brattleboro rat to synthesize vasopressin and vasopressin-associated neurophysin. Cell Tissue Res 165:307–316
Watson SJ, Akil H, Fischli W, Goldstein A, Zimmerman E, Nilaver G, Van Wiersma Greidanus TB (1982) Dynorphin and vaso- pressin: common localization in magnocellular neurons. Science 216:85–87
Weibel ER (1973) Stereological techniques for electron microscopic morphometry. In: Hayat MA (ed) Principles and techniques of electron microscopy. Biological applications, vol 3, Van Nostrand Reinhold, New York, pp 239–296
Williams MA (1977) Quantitative methods in biology, vol 6 of Practical methods in electron microscopy (ed AM Glauert). North Holland Publishing Company, Amsterdam, pp 54–60
Whitnall MH, Gainer H, Cox BM, Molineaux CJ (1983) Dynorphin-A-(1–8) is contained within vasopressin neurosecretory vesicles in rat pituitary. Science 222:1137–1139
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Chapman, D.B., Morris, J.F. Granule populations in oxytocin and abnormal perikarya of the supraoptic nucleus of homozygous Brattleboro rats: Effects of colchicine administration. Cell Tissue Res. 241, 435–444 (1985). https://doi.org/10.1007/BF00217191
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DOI: https://doi.org/10.1007/BF00217191