Summary
The popliteal lymph nodes were removed from young rats of various ages five days after a single immunization with TNP-KLH in the hind footpads. Cryostat sections of the lymph nodes were investigated by means of enzyme and immunohistochemical techniques at the light-microscopical level.
The presence and localization of anti-TNP antibody-containing cells were examined using a new technique to visualize specific antibodies. Moreover, the development of the lymph nodes following exogenous antigenic stimulation was compared with that of unstimulated lymph nodes.
Specific antibody-containing cells could not be found before day 15 after birth, in rats immunized at day 10. From that time these lymphoid cells were located primarily at the border between cortex and medulla. Younger popliteal lymph nodes showed only aspecific immunoglobulin-containing lymphoid cells. With age, the number of specific antibody-containing cells tended to increase. These cells were more mature, according to morphological criteria and were located nearer the medulla.
The first primary follicles were seen at day 19, as was the case in unstimulated animals. The first secondary follicles, containing germinal centers, were detected at day 23, whereas in unstimulated popliteal lymph nodes they were never found.
Trapping of immune complexes could not be demonstrated before day 33 after birth. The later appearance of this phenomenon might be a consequence of the techniques applied to demonstrate specific antibody-containing cells.
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Abbreviations
- PLN:
-
popliteal lymph node
- FDC:
-
follicular dendritic cell
- IDC:
-
interdigitating cell
- HEV:
-
high endothelial venule
- TNP:
-
trinitrophenyl
- KLH:
-
keyhole limpet hemocyanin
- PBS:
-
phosphate-buffered saline
- GCPC:
-
germinal center precursor cell
- sIg :
-
surface immunoglobulin
- cIg :
-
cytoplasmic immunoglobulin
References
Barclay AN (1981) Different reticular cells in rat lymphoid tissue identified by localization of Ia, Thy-1 and MRC Ox-2 antigens. Immunology 44:727–735
Brachet J (1953) The use of dyes and ribonuclease for the cytochemical detection of ribonucleic acid. J Microsc Sci 94:1–10
Burstone MS (1958) Histochemical comparison of naphthol phosphates for the demonstration of phosphatases. J Natl Cancer Inst 20:601–616
Claassen E, Van Rooijen N (1984) TNP-enzyme conjugates for the detection of anti-TNP antibody containing cells in vivo. J Immunol Methods 75:181–188
Decker JM, Clarke J, Bradley LM, Miller A, Sercarz EE (1974) Presence of antigen binding cells for five diverse antigens at the onset of lymphoid development: lack of evidence for somatic diversification during ontogeny. J Immunol 113:1823–1833
Deenen GJ, Opstelten D, Nieuwenhuis P (1984) Homing of germinal center cells into germinal centers of lymph node via afferent lymphatics. Cell Tissue Res 238:183–189
Dijkstra CD, Van Tilburg NJ, Döpp EA (1982) Ontogenetic aspects of immune complex trapping in the spleen and popliteal lymph nodes of the rat. Cell Tissue Res 223:545–552
Dijkstra CD, Kamperdijk EWA, Döpp EA (1984) The ontogenetic development of the follicular dendritic cell. Cell Tissue Res 236:203–206
Eikelenboom P, Nassy JJJ, Post J, Versteeg JCMB, Langevoort HL (1978) The histogenesis of lymph nodes in rat and rabbit. Anat Rec 190:201–216
Etlinger HM, Chiller JM (1983) Maturation of the lymphoid system. IV. Ontogenetic compartmentalization of T cell function. Dev Comp Immunol 7:333–346
Gastkemper NA, Wubbena AS, Guinbrère FJH, De Graaff A, Nieuwenhuis P (1981) Germinal centers and the B cell system. V. Presence of germinal centre-precursor cells among lymphocytes of the thoracic duct in the rat. Cell Tissue Res 219:281–289
Geldof AA, Rijnhart P, Van de Ende M, Kors N, Langevoort HL (1984) Morphology, kinetics and secretory activity of antibody forming cells. Immunobiology 166:296–307
Goidl EA, Siskind GW (1974) Ontogeny of B lymphocyte function. I. Restricted heterogeneity of the antibody response of B lymphocytes from neonatal and fetal mice. J Exp Med 140:1285–1302
Graham RC, Karnovsky MC (1966) The early stages of absorption of injected horseradish peroxidase in the proximal tubules of mouse kidney: ultrastructural cytochemistry by a new technique. J Histochem Cytochem 14:291–302
Haaijman JJ, Spedding Micklem H, Ledbetter JA, Dangl JL, Her- zenberg LA, Herzenberg LA (1981) T cell ontogeny: organ location of maturing populations as defined by surface antigen markers is similar in neonates and adults. J Exp Med 153:605–614
Inaba K, Masuda T, Mayama-Inaba M, Aotsuka Y, Kura F, Ko- matsu-Bara S, Ido M, Muramatsu S (1982) Ontogeny of macrophage function. III. Manifestation of high accessory cell activity for primary antibody response by Ia+ functional cells in newborn mouse spleen in collaboration with Ia macrophages. Immunology 47:449–457
Kimura S, Eldridge JH, Michalek SM, Morisake I, Hamada S, McGhee JR (1985) Immunoregulation in the rat: ontogeny of B cell responses to types 1, 2 and T-dependent antigens. J Immunol 134:2839–2846
Mosier DE, Johnson BM (1975) Ontogeny of mouse lymphocyte function. II. Development of the ability to produce antibody is modulated by T lymphocytes. J Exp Med 141:216–226
Nadler PL, Klingenstein RJ, Hodes RJ (1980) Ontogeny of murine accessory cells: Ia antigen expression and accessory cell function in in vitro primary antibody responses. J Immunol 25:914–920
Nieuwenhuis P, Opstelten D (1984) Functional anatomy of germinal centers. Am J Anat 170:427–435
Pearse AGE (1968) Histochemistry. Theoretical and applied. Vol I, 3rd ed. Churchill Livingstone, Edinburgh
Rozing J, Brons NHC, Van Ewijk W, Benner R (1978) Blymphocyte differentiation in lethally irradiated and reconstituted mice. A histological study using immunofluorescent detection of B lymphocytes. Cell Tissue Res 189:19–30
Schultz RD, Dunne HW, Heist CE (1973) Ontogeny of the bovine immune response. Infect Immun 7:981–991
Stet RJM, Rozing J, Majoor GD, Kroese FGM, Opstelten D, Nieuwenhuis P (1985) His 19: a monoclonal antibody recognizing a class II polymorphic determinant only absent on RT1 n class-II antigens. Transplant Proc 17:1829–1831
Tew JG, Mandel TE, Miller GA (1979) Immune retention. Immunological requirements for maintaining an easily degradable antigen in vivo. Aust J Exp Biol Med Sci 57:401–414
Thorbecke GJ, Romano TJ, Lerman SP (1974) Regulatory mechanisms in proliferation and differentiation of lymphoid tissue, with particular reference to germinal centre development. Prog Immunol II 3:25–34
Vaessen LMB, Joling P, Nieuwenhuis P, Van Haperen MJ, Rozing J (1985) Tissue markers on pre-B cells in the rat. Adv Exp Med Biol 186:17–26
Van Rees EP, Döpp EA, Dijkstra CD, Sminia T (1985) The postnatal development of cell populations in the rat popliteal lymph node. An immunohistochemical study. Cell Tissue Res 242:391–398
Van Rooijen N, Kors N, Van Nieuwmegen R (1984) Double immunocytochemical evidence for a clonal development of specific antibody-containing cells in the rabbit spleen. Anat Rec 209:385–390
Van Rooijen N, Van Nieuwmegen R, Kors N (1985) The influence of the route of antigen administration on the development of specific antibody-producing cells in the follicles of the popliteal lymph nodes of rabbits. Cell Tissue Res 239:657–660
Veerman AJP (1975) The postnatal development of the white pulp in the rat spleen and the onset of immunocompetence against a thymus independent and a thymus dependent antigen. Z Immun Forsch 150:45–59
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van Rees, E.P., Dijkstra, C.D. & van Rooijen, N. The early postnatal development of the primary immune response in TNP-KLH-stimulated popliteal lymph node in the rat. Cell Tissue Res. 246, 673–677 (1986). https://doi.org/10.1007/BF00215210
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DOI: https://doi.org/10.1007/BF00215210