Summary
Adenylate cyclase activity was localized in various tissues of the early chick embryo using an ultrastructural histochemical technique. Reaction product was deposited on the lateral plasma membrane of all cells, but with a preferential localization at the apical terminal complex in the epiblast. There was no activity associated with the free surfaces of these or other cells in the embryo. Intracellular deposits were found in all cells associated with the endoplasmic reticulum, nuclear envelope and Golgi bodies. In the last organelle, the deposit was sometimes observed to be distributed through the stack in a non-uniform way, with the heaviest deposits occurring at the forming face. No clear difference could be detected between the cytochemical activity associated with cells in various regions of the embryo, or with embryos at different stages of early development.
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References
Bellairs R, Breathnach AS, Gross M (1975) Freeze-fracture replication of junctional complexes in unincubated and incubated chick embryos. Cell Tissue Res 162:235–252
Cheng H, Farquhar MG (1976) Presence of adenylate cyclase activity in Golgi and other fractions from rat liver. II Cytochemical localization within Golgi and ER membranes. J Cell Biol 70:671–684
Cutler LS (1983) Cytochemical methods for the localization of adenylate cyclase. A review and evaluation of the efficacy of the procedures. J Histochem Cytochem 31:85–93
Cutler LS, Christian CP, Feinstein MB (1985) Cytochemical localization of adenylate cyclase in the dense tubule system of human blood platelets stimulated by forskolin, prostacyclin and prostaglandin D2. Biochim Biophys Acta 845:403–40
Dini G, Del Rosso M (1983) Differences of adenylate cyclase localization in guinea-pig peritoneal macrophages under different physiological conditions: an ultracytochemical study. Histochem J 15:911–918
Dunlap MK (1980) Cyclic AMP levels in migrating and non-migrating newt epidermal cells. J Cell Physiol 104:367–373
Dunlap MK, Donaldson DJ (1980) Effect of cAMP and related compounds on newt epidermal cell migration both in vivo and in vitro. J Exp Zool 212:13–19
Gingle AR (1977) cAMP enhanced aggregation of cells from early chick embryo. Dev Biol 58:394–401
Gingle AR, Robertson A (1979) Responses of the early chick embryo to external cAMP sources. J Embryol Exp Morphol 53:353–365
Greene RM, Pratt RM (1979) Correlation between cyclic-AMP levels and cytochemical localization of adenylate cyclase during development of the secondary palate. J Histochem Cytochem 27:924–931
Hamburger V, Hamilton HL (1951) A series of normal stages in the development of the chick embryo. J Morphol 88:49–92
Harris HL, Zalik SE (1974) Studies on the surface of chick blastoderm cells. III. Calcium binding to ionogcnic sites. J Cell Physiol 83:359–368
Katzin WE, Gersham H (1984) Control of cell mobility by cyclic AMP. J Cell Sci 65:177–192
Kempen HJM, de Pont JJHHM, Bonting SL, Stadhouders, AM (1978) The cytochemical localization of adenylate cyclase: fact or artifact? J Histochem Cytochem 26:298–312
Kim S-K (1976) The cytochemical localization of adenylate cyclase activity in mucous and serous cells of the salivary gland. J Supramol Struct 4:185–197
Kuroda Y (1974) Inhibition by cyclic AMP and dibutyryl cyclic AMP of aggregation of embryonic quail liver cells in culture. Exp Cell Res 84:303–310
Milos N, Zalik SE (1981) Effect of the β-D-galactoside-binding lectin on cell-to-substratum and cell-to-cell adhesion of cells from the extraembryonic endoderm of the early chick blastoderm. Wilhelm Rouxs Arch 190:259–266
Neuman T, Laasberg T, Karner J (1983) Ultrastructural localization of adenylate cyclase and cAMP phosphodiesterase in the gastrulating chick embryo. Rouxs Arch Dev Biol 192:42–44
Newgreen DF, Gooday D (1985) Control of the onset of migration of neural crest cells in avian embryos. Role of Ca++-dependent cell adhesions. Cell Tissue Res 239:329–336
Poeggel G, Luppa H, Bernstein HG, Weiss J (1984) Histochemistry of adenylate cyclase. Int Rev Cytol 89:35–64
Reporter M, Rosenquist GC (1972) Adenosine 3′,5′-monophosphate. Regional differences in chick embryos at the head process stage. Science 178:628–630
Robertson A, Grutsch JF, Gingle AR (1978) Cyclic adenosine monophosphate production by embryonic chick cells. Science 199:990–991
Sanders EJ (1973) Intercellular contact in the unincubated chick embryo. Z Zellforsch 141:459–468
Sanders EJ (1980) The Golgi body and thiamine pyrophosphatase localization in embryonic cells in culture. Exp Cell Res 126:458–461
Schulze W, Hinterberger U, Wollenberger A, Krause E-G, Janiszewski E (1977) Problems of the cytochemical demonstration of adenylate cyclase. Acta Histochem Cytochem 10:371–378
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Sanders, E.J. Ultrastructural cytochemical localization of adenylate cyclase in the early chick embryo. Cell Tissue Res. 247, 465–468 (1987). https://doi.org/10.1007/BF00218330
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DOI: https://doi.org/10.1007/BF00218330