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Orthogonal arrays of particles in non-pigmented cells of rat ciliary epithelium: Relation to distribution of filipin- and digitonin-induced alterations of the basolateral membrane

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Summary

It has been suggested that orthogonal arrays of particles may increase the rigidity of plasma membrane, as does cholesterol. Therefore, using freeze-fractured non- pigmented ciliary epithelium, the distribution of such arrays was compared to the distribution of membrane deformations induced by the sterol-probes filipin and digitonin in different domains of the basolateral plasma membrane. The distribution of orthogonal arrays of particles was homogeneous between different regions of the basolateral membrane of the non-pigmented ciliary epithelium, while the number of filipin-induced alterations was nearly 4 times higher in the membrane domains not in contact with the basal lamina than in domains in contact with it. Contrary to the homogeneous distribution of arrays, digitonin-induced deformations also differed markedly in these two basolateral membrane domains. Considering that a marked positive response to sterol probes implies a high sterol content, we conclude that orthogonal arrays of particles can occur in plasma membrane regions well-provided with cholesterol and not in direct contact with the basal lamina. Other possible roles of these arrays are discussed.

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Author notes

  1. Walter Noske

    Present address: Augen- und Poliklinik der Freien Universität, Klinikum Steglitz, Hindenburgdamm 30, D-1000, Berlin 45, FRG

Authors and Affiliations

  1. Laboratoire de Technologie Appliquée à la Microscopie Electronique, CNRS, Paris, France

    Michel Hirsch, Danièle Gache & Walter Noske

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  1. Michel Hirsch
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  2. Danièle Gache
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  3. Walter Noske
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Hirsch, M., Gache, D. & Noske, W. Orthogonal arrays of particles in non-pigmented cells of rat ciliary epithelium: Relation to distribution of filipin- and digitonin-induced alterations of the basolateral membrane. Cell Tissue Res. 252, 165–173 (1988). https://doi.org/10.1007/BF00213838

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