Summary
An ultrastructural morphometric study of the endocrine cells of the oxyntic mucosa of the stomach in gastric biopsies collected from five male and five female healthy volunteers aged 19–31 was performed. No sex-related differences were disclosed. Endocrine cells accounted for 1.2±0.4% of the epithelial volume and 0.9±0.4% of the mucosal volume, i.e., including the lamina propria. After classification of the specific endocrine cell types according to the ultrastructural morphology of secretory granules, the volume densities of ECL, P and D cells (30±9%, 24±7%, and 22±4% of the entire endocrine cell mass, respectively) were higher than those of other endocrine cell types. In particular, EC cells contributed less than 10% and X cells represented a very low proportion of the total cells. Non-granulated profiles of cells which in all other respects appeared to be endocrine were also found with a volume density of 8±4%. D cells were distinguished by the high fraction of cytoplasm occupied by secretory granules (31±5%). Subdivision of the whole mucosa into four horizontal segments revealed the endocrine cells to be mostly distributed in the three lower, with virtually no endocrine cells in the superficial segment. The quantitative ultrastructural analysis of the endocrine cell population of the normal human oxyntic mucosa provided by this study may allow a better evaluation of physiological and pharmacological variations of the endocrine cell population.
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References
Black WC (1968) Enterochromaffin cell types and corresponding carcinoid tumors. Lab Invest 19:473–486
Bordi C, Costa A, Missale G (1975) ECL cell proliferation and gastrin levels. Gastroenterology 68:205–206
Bordi C, Ravazzola M, De Vita O (1983) Pathology of endocrine cells in gastric mucosa. Ann Pathol 3:19–28
Bordi C, Bertelé A, Davighi MC, Pilato F, Carfagna G, Missale G (1984) Clinical and pathological associations of argyrophil cell hyperplasias of the gastric mucosa. Appl Pathol 2:282–291
Bordi C, Ferrari C, D'Adda T, Pilato FP, Carfagna G, Bertelé A, Missale G (1986a) Ultrastructural characterization of fundic endocrine cell hyperplasia associated with atrophic gastritis and hypergastrinemia. Virchows Arch [A] 409:335–347
Bordi C, Pilato FP, Carfagna G, Ferrari C, D'Adda T, Sivelli R, Bertelé A, Missale G (1986b) Argyrophil cell hyperplasia of fundic mucosa in patients with chronic atrophic gastritis. Digestion Suppl 35, 1:130–143
Bordi C, D'Adda T, Pilato FP, Ferrari C (1988) Carcinoid (ECL cell) tumor of the oxyntic mucosa of the stomach: A hormonedependent neoplasm? In: Fenoglio-Preiser C, Wolff M, Rilke F (eds) Progress in Surgical Pathology. Field and Wood, Philadelphia, Vol. 8, pp 177–195
Capella C, Vassallo G, Solcia E (1971) Light and electron microscopic identification of the histamine-storing argyrophil (ECL) cell in murine stomach and of its equivalent in other mammals. Z Zellforsch 118:68–84
Capella C, Hage E, Solcia E, Usellini L (1978) Ultrastructural similarity of endocrine like cells of the human lung and some related cells of the gut. Cell Tissue Res 186:25–37
D'Adda T, Bordi C (1988) Ultrastructure of a neuroendocrine complex in oxyntic mucosa of normal human stomach. Cell Tissue Res (in press)
De Lemos C (1977) The ultrastructure of endocrine cells in the corpus of human fetuses. Am J Anat 148:359–384
Ekman L, Hansson E, Havu N, Carlsson E, Lundberg C (1985) Toxicological studies on omeprazole. Scand J Gastroenterol Suppl 20, 108:53–69
Grube D, Forssmann WG (1979) Morphology and function of the entero-endocrine cells. Horm Metab Res 11:589–606
Hakanson R, Ekelund M, Sundler F (1984) Activation and proliferation of gastric endocrine cells. In: Falkmer S, Hakanson R, Sundler F (eds) Evolution and Tumor Pathology of the Neuroendocrine System. Elsevier, Amsterdam, pp 371–398
Hakanson R, Bottcher G, Ekblad E, Panula P, Simonsson M, Dohlsten M, Hallberg T, Sundler F (1986) Histamine in endocrine cells in the stomach. A survey of several species using a panel of histamine antibodies. Histochemistry 86:5–17
Helander HF (1981) The cells of the gastric mucosa. Int Rev Cytol 70:217–289
Helander HF, Leth R, Olbe L (1986) Stereological investigations on human gastric mucosa: I. Normal oxyntic mucosa. Anat Rec 216:373–380
Karnovsky MJ (1963) A formaldehyde-glutaraldehyde fixative of high osmolality for use in electron microscopy. J Cell Biol 27:137a
Kobayashi S, Fujita T, Sasagawa T (1971) Electron microscope studies on the endocrine cells of the human gastric fundus. Arch Histol Jpn 32:429–444
Larsson LI (1980) Peptide secretory pathways in GI tract: cytochemical contributions to regulatory physiology of the gut. Am J Physiol 239:G237-G246
Larsson H, Carlsson E, Mattsson H, Lundell L, Sundler F, Sundell G, Wallmark B, Watanabe T, Hakanson R (1986) Plasma gastrin and gastric entero-chromaffin like cell activation and proliferation. Studies with omeprazole and ranitidine in intact and antrectomized rats. Gastroenterology 90:391–399
Poynter D, Pick CR, Harcourt RA, Selway SAM, Ainge G, Harman IW, Spurung NW, Fluck PA, Cook JL (1985) Association of long lasting unsurmountable histamine H-2 blockade and gastric carcinoid tumors in the rat. Gut 26:1284–1295
Poynter D, Selway SAM, Papworth SA, Riches SR (1986) Changes in the gastric mucosa of the mouse associated with long lasting unsurmountable histamine H-2 blockade. Gut 27:1338–1346
Rindi G, Buffa R, Sessa F, Tortora O, Solcia E (1986) Chromogranin A, B and C immunoreactivities of mammalian endocrine cells. Distribution, distinction from costored hormones/prohormones and relationship with argyrophil component of secretory granules. Histochemistry 85:19–28
Solcia E, Capella C, Vassallo G, Buffa R (1975) Endocrine cells of the gastric mucosa. Int Rev Cytol 42:223–286
Solcia E, Capella C, Buffa R, Usellini L, Frigerio B, Fontana P (1979) Endocrine cells of the gastrointestinal tract and related tumors. Pathobiol Annu 9:163–204
Solcia E, Capella C, Buffa R, Frigerio B, Sessa F, Tenti P (1981) Histopathology and cytology of gastroenteropancreatic endocrine tumors. In: Friedman M, Ogawa M, Kisner D (eds) Diagnosis and Treatment of Upper Gastrointestinal Tumors. Excerpta Medica, Amsterdam, pp 32–51
Solcia E, Capella C, Sessa F, Rindi G, Cornaggia M (1986) Gastric carcinoids and related endocrine growths. Digestion Suppl 35: 1, 3–22
Wallenstein S, Zucker CL, Fleiss JL (1980) Some statistical methods useful in circulation research. Circ Res 47:1–9
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D'Adda, T., Bertelé, A., Pilato, F.P. et al. Quantitative electron microscopy of endocrine cells in oxyntic mucosa of normal human stomach. Cell Tissue Res. 255, 41–48 (1989). https://doi.org/10.1007/BF00229064
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DOI: https://doi.org/10.1007/BF00229064