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Cellular distribution of parvalbumin immunoreactivity in the peripheral vestibular system of three rodents

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Abstract

The cellular distribution of parvalbumin immunoreactivity in the vestibular peripheral system of mouse, rat, and guinea pig was investigated by light and electron microscopy. Parvalbumin was found in all neurons of the vestibular ganglia of these species but in the sensory epithelia immunoreactivity was restricted to type I hair cells localized exclusively in the central areas. The very intense staining pattern was similar in the cristae ampullares and utricles of all three species but a faint immunoreaction was also detectable in sensory cells of peripheral areas of rat cristae. The parvalbumin-immunoreactive type I sensory cells are connected by nerve fibres of the calyx unit type which are known selectively to contain calretinin.

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References

  • Baird RA, Desmadryl G, Fernandez C, Goldberg JM (1988) The vestibular nerve of the chinchilla. II. Relation between afferent response properties and peripheral innervation patterns in the semicircular canals. J Neurophysiol 60:182–203

    Google Scholar 

  • Braun K, Scheich H, Schachner M, Heizmann CW (1985a) Distribution of parvalbumin, cytochrome oxidase activity and (14C)2-deoxyglucose uptake in the brain of the zebra finch. I. Auditory and vocal motor systems. Cell Tissue Res 240:101–115

    Google Scholar 

  • Braun K, Scheich H, Schachner M, Heizmann CW (1985b) Distribution of parvalbumin, cytochrome oxidase activity and (14C)2-deoxyglucose uptake in the brain of zebra finch. II. Visual system. Cell Tissue Res 240:117–127

    Google Scholar 

  • Celio MR (1986) Parvalbumin in most gamma-aminobutyric acid containing neurons of the rat cerebral cortex. Science 231:995–997

    Google Scholar 

  • Celio MR (1988) Monoclonal antibodies directed against the calcium binding protein parvalbumin. Cell Calcium 9:81–88

    Google Scholar 

  • Celio MR (1990) Calbindin D-28 k and parvalbumin in the rat nervous system. Neuroscience 35:375–475

    Google Scholar 

  • Celio MR, Heizmann CW (1981) Calcium-binding protein parvalbumin as a neuronal marker. Nature 293:300–302

    Google Scholar 

  • Côté P-Y, Sadikot AF, Parent A (1991) Complementary distribution of calbindin D-28k and parvalbumin in the basal forebrain and midbrain of the squirrel monkey. Eur J Neurosci 3:1316–1320

    Google Scholar 

  • Dechesne CJ, Thomasset M (1988a) Calbindin (CaBP 28 kDa) appearance and distribution during development of the mouse inner ear. Dev Brain Res 40:233–242

    Google Scholar 

  • Dechesne CJ, Thomasset M, Brehier A, Sans A (1988b) Calbindin (CaBP 28 KDa) localization in the peripheral vestibular system of various vertebrates. Hearing Res 33:273–278

    Google Scholar 

  • Dechesne CJ, Hampson DR, Goping G, Wheaton KD, Wenthold RJ (1991a) Identification and localization of a kainate binding protein in the frog inner ear by electron microscopy immunocytochemistry. Brain Res 545:223–233

    Google Scholar 

  • Dechesne CJ, Winsky L, Kim HN, Goping G, Vu TD, Wenthold RJ, Jacobowitz DM (1991b) Identification and ultrastructural localization of a calretinin-like calcium binding protein (protein 10) in the guinea pig and rat inner ear. Brain Res 560:139–148

    Google Scholar 

  • Demêmes D, Wenthold RJ, Moniot B, Sans A (1990) Glutamate-like immunoreactivity in the peripheral vestibular system of mammals. Hearing Res 46:261–270

    Google Scholar 

  • Demêmes D, Moniot B, Lomri N, Thomasset M, Sans A (1991) Detection of calbindin-D 28k mRNA in rat vestibular ganglion neurons by in situ hybridization. Mol Brain Res 9:153–156

    Google Scholar 

  • Demêmes D, Raymond J, Atger P, Grill C, Winsky L, Dechesne CJ (1992) Identification of neuron subpopulations in the rat vestibular ganglion by calbindin-D 28K, calretinin and neurofilament protein immunoreactivity. Brain Res 582:168–172

    Google Scholar 

  • Desmadryl G, Dechesne CJ (1992) Calretinin immunoreactivity in chinchilla and guinea pig vestibular end organs characterizes the calyx unit subpopulation. Exp Brain Res 89:105–108

    Google Scholar 

  • Endo T, Kobayashi S, Onaya T (1985) Parvalbumin in rat cerebrum, cerebellum and retina during postnatal development. Neurosci Lett 60:279–282

    Google Scholar 

  • Endo T, Kobayashi M, Kobayashi S, Onaya T (1986a) Immunocytochemical and biochemical localization of parvalbumin in the retina. Cell Tissue Res 243:213–217

    Google Scholar 

  • Endo T, Takazawa K, Kobayashi S, Onaya T (1986b) Immunocytochemical and immunohistochemical localization of parvalbumin in rat nervous tissues. J Neurochem 46:892–898

    Google Scholar 

  • Eybalin M, Ripoll C (1990) Immunolocalisation of parvalbumin in two glutamatergic cell types of the guinea pig cochlea: the inner hair cells and spiral ganglion neurons. C R Acad Sci (III) 310:639–644

    Google Scholar 

  • Fernandez C, Baird RA, Goldberg JM (1988) The vestibular nerve of the chinchilla. I. Peripheral innervation patterns in the horizontal and superior semicircular canals. J Neurophysiol 60:176–181

    Google Scholar 

  • Fernandez C, Goldberg JM, Baird RA (1990) The vestibular nerve of the chinchilla. III. Peripheral innervation patterns in the utricular macula. J Neurophysiol 63:767–780

    Google Scholar 

  • Goldberg JM, Desmadryl G, Baird RA, Fernández C (1990) The vestibular nerve of the Chinchilla. V. Relation between afferent discharge properties and peripheral innervation patterns in the utricular macula. J Neurophysiol 63:791–804

    Google Scholar 

  • Hacohen N, Assad JA, Smith WJ, Corey DP (1989) Regulation of tension on hair-cell transduction channels: Displacement and calcium dependence. J Neurosci 9:3988–3997

    Google Scholar 

  • Heizmann CW (1984) Parvalbumin, an intracellular calcium-binding protein: distribution, properties and possible role in mammalian cells. Experientia 40:910–921

    Google Scholar 

  • Heizmann CW, Braun K (1990) Calcium-binding proteins. Molecular and functional aspects. In: L.J. Anghileri (ed). The Role of Calcium in Biological Systems, vol 5. CRC Press, pp 21–66

  • Heizmann CW, Celio MR (1987) Immunolocalization of parvalbumin. Meth Enzymol 39:552–570

    Google Scholar 

  • Hudspeth AJ, Lewis RS (1988a) Kinetic analysis of voltage- and ion-dependent conductances in saccular hair cells of the bull-frog, Rana catesbeiana. J Physiol (Lond) 400:237–274

    Google Scholar 

  • Hudspeth AJ, Lewis RS (1988b) A model for electrical resonance and frequency tuning in saccular hair cells of the bull-frog, Rana catesbeiana. J Physiol (Lond) 400:275–297

    Google Scholar 

  • Kawaguchi Y, Katsumaru H, Kosaka T, Heizmann CW, Hama K (1987) Fast-spiking cells in rat hippocampus (CA1-region) contain the calcium-binding protein parvalbumin. Brain Res 416:369–374

    Google Scholar 

  • Kosaka T, Katsumaru H, Hama K, Wu J-Y, Heizmann CW (1987) Gabaergic neurons containing the calcium binding protein parvalbumin in the rat hippocampus and dentate gyrus. Brain Res 419:119–130

    Google Scholar 

  • Lopez I, Juiz JM, Altschuler RA, Meza G (1990) Distribution of GABA-like immunoreactivity in guinea pig vestibular cristae ampullaris. Brain Res 530:170–175

    Google Scholar 

  • Morris RJ, Beech JN, Heizmann CW (1988) Two distinct phases and mechanisms of axonal growth shown by primary vestibular fibres in the brain demonstrated by parvalbumin immunocytochemistry. Neuroscience 27:571–596

    Google Scholar 

  • Ohm TG, Muller H, Braak E (1991) Calbindin-D-28k-like immunoreactive structures in the olfactory bulb and anterior olfactory nucleus of the human adult: distribution and cell typologypartial complementarity with parvalbumin. Neuroscience 42:823–840

    Google Scholar 

  • Ohmori H (1985) Mechano-electrical transduction currents in isolated vestibular hair cells in the chick. J Physiol (Lond) 359:189–217

    Google Scholar 

  • Raymond J, Demêmes D, Nieoullon A (1984) Neurotransmitters in vestibular pathways. In: Pompeiano O, Allum JHJ (eds) Vestibulospinal control of posture and locomotion. Progress in brain Res, vol 76. Elsevier, Amsterdam, 29–43

    Google Scholar 

  • Raymond J, Dechesne DJ, Desmadryl G, Demêmes D (1993) Different calcium-binding proteins identify subpopulations of vestibular ganglion neurons in the rat. Acta Otolaryngol 503 [Suppl]:114–118

    Google Scholar 

  • Röhrenbeck J, Wässle H, Heizmann CW (1987) Immunocytochemical labelling of horizontal cells in mammalian retina using antibodies against calcium-binding proteins. Neurosci Lett 77:255–260

    Google Scholar 

  • Sans A, Etchecopar B, Brehier A, Thomasset M (1986) Immunocytochemical detection of vitamin D-dependent calcium binding protein (CaBP-28K) in vestibular sensory hair cells and vestibular ganglion neurones of the cat. Brain Res 364:190–194

    Google Scholar 

  • Sans A, Brehier A, Moniot B, Thomasset M (1987) Immuno-electronmicroscopic localization of vitamin D-dependent calcium-binding protein (CaBP 28k) in the vestibular hair cells of the cat. Brain Res 435:293–304

    Google Scholar 

  • Scarfone E, Demêmes D, Jahn R, De Camilli P, Sans A (1988) Secretory function of the vestibular nerve calyx suggested by presence of vesicles, synapsin and synaptophysin. J Neurosci 8:4640–4645

    Google Scholar 

  • Stichel CC, Singer W, Heizmann CW (1987) Immunohistochemical localization of calcium-binding proteins, parvalbumin and calbindin D 28K, in the adult and developing visual cortex of cats: a light and electron microscopic study. J Comp Neurol 262:563–577

    Google Scholar 

  • Stichel CC, Singer W, Heizmann CW (1988) Light and electron microscopic immunocytochemical localization of parvalbumin in the dorsal lateral geniculate nucleus of the cat: evidence for coexistence with GABA. J Comp Neurol 268:29–37

    Google Scholar 

  • Tinner R, Oertle M, Heizmann CW, Bosshard HR (1990) Ca2+-binding site of carp parvalbumin recognized by monoclonal antibody. Cell Calcium 11:19–23

    Google Scholar 

  • Wersäll J, Bagger-Sjöbäck D (1974) Morphology of the vestibular sense organs. In: Kornhuber HH (ed) Handbook of Sensory Physiology, vol VI/1. Springer. New York, pp 123–171

    Google Scholar 

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Authors and Affiliations

  1. INSERM U 254, Laboratoire de Neurophysiologie Sensorielle, USTL, Place E. Bataillon, F-34095, Montpellier Cedex 05, France

    Danielle Demêmes

  2. INSERM U 254, Laboratoire de Neurobiologie de l'Audition, C.H.U., Hôpital St. Charles, F-34059, Montpellier Cedex 1, France

    Michel Eybalin & Nicole Renard

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  1. Danielle Demêmes
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  2. Michel Eybalin
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  3. Nicole Renard
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Demêmes, D., Eybalin, M. & Renard, N. Cellular distribution of parvalbumin immunoreactivity in the peripheral vestibular system of three rodents. Cell Tissue Res 274, 487–492 (1993). https://doi.org/10.1007/BF00314545

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  • DOI: https://doi.org/10.1007/BF00314545

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